Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31220 and RR42_RS31225 overlap by 4 nucleotides RR42_RS31225 and RR42_RS31230 are separated by 91 nucleotides RR42_RS31230 and RR42_RS31235 overlap by 4 nucleotides RR42_RS31235 and RR42_RS31240 overlap by 8 nucleotides
RR42_RS31220: RR42_RS31220 - lipoprotein carrier protein LolA, at 2,382,247 to 2,382,873
_RS31220
RR42_RS31225: RR42_RS31225 - 4-hydroxybenzoyl-CoA thioesterase, at 2,382,870 to 2,383,319
_RS31225
RR42_RS31230: RR42_RS31230 - acyltransferase, at 2,383,411 to 2,384,367
_RS31230
RR42_RS31235: RR42_RS31235 - glycosyl transferase, at 2,384,364 to 2,385,107
_RS31235
RR42_RS31240: RR42_RS31240 - AMP-dependent synthetase, at 2,385,100 to 2,386,818
_RS31240
Position (kb)
2383
2384
2385 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2382.940 kb on + strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.986 kb on - strand, within RR42_RS31225 at 2382.986 kb on - strand, within RR42_RS31225 at 2382.986 kb on - strand, within RR42_RS31225 at 2383.030 kb on + strand, within RR42_RS31225 at 2383.093 kb on + strand, within RR42_RS31225 at 2383.094 kb on - strand, within RR42_RS31225 at 2383.098 kb on - strand, within RR42_RS31225 at 2383.098 kb on - strand, within RR42_RS31225 at 2383.111 kb on + strand, within RR42_RS31225 at 2383.111 kb on + strand, within RR42_RS31225 at 2383.111 kb on + strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.144 kb on + strand, within RR42_RS31225 at 2383.174 kb on + strand, within RR42_RS31225 at 2383.174 kb on + strand, within RR42_RS31225 at 2383.225 kb on + strand, within RR42_RS31225 at 2383.225 kb on + strand, within RR42_RS31225 at 2383.226 kb on - strand, within RR42_RS31225 at 2383.319 kb on + strand at 2383.320 kb on - strand at 2383.320 kb on - strand at 2383.421 kb on + strand at 2383.631 kb on + strand, within RR42_RS31230 at 2384.015 kb on + strand, within RR42_RS31230 at 2384.021 kb on + strand, within RR42_RS31230 at 2384.050 kb on + strand, within RR42_RS31230 at 2384.050 kb on + strand, within RR42_RS31230 at 2384.051 kb on - strand, within RR42_RS31230 at 2384.156 kb on + strand, within RR42_RS31230 at 2384.198 kb on + strand, within RR42_RS31230 at 2384.198 kb on + strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.255 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.284 kb on + strand at 2384.432 kb on + strand at 2384.432 kb on + strand at 2384.433 kb on - strand at 2384.497 kb on + strand, within RR42_RS31235 at 2384.497 kb on + strand, within RR42_RS31235 at 2384.498 kb on - strand, within RR42_RS31235 at 2384.515 kb on - strand, within RR42_RS31235 at 2384.629 kb on + strand, within RR42_RS31235 at 2384.630 kb on - strand, within RR42_RS31235 at 2384.705 kb on - strand, within RR42_RS31235 at 2384.855 kb on + strand, within RR42_RS31235 at 2385.077 kb on - strand at 2385.077 kb on - strand at 2385.101 kb on + strand at 2385.102 kb on - strand at 2385.102 kb on - strand at 2385.102 kb on - strand at 2385.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,382,940 + RR42_RS31225 0.16 -0.6 2,382,941 - RR42_RS31225 0.16 -0.1 2,382,941 - RR42_RS31225 0.16 -1.1 2,382,941 - RR42_RS31225 0.16 -0.5 2,382,941 - RR42_RS31225 0.16 -0.7 2,382,986 - RR42_RS31225 0.26 -1.3 2,382,986 - RR42_RS31225 0.26 -0.3 2,382,986 - RR42_RS31225 0.26 -3.9 2,383,030 + RR42_RS31225 0.36 -2.5 2,383,093 + RR42_RS31225 0.50 +0.4 2,383,094 - RR42_RS31225 0.50 -0.1 2,383,098 - RR42_RS31225 0.51 +0.3 2,383,098 - RR42_RS31225 0.51 -2.7 2,383,111 + RR42_RS31225 0.54 +0.6 2,383,111 + RR42_RS31225 0.54 -0.9 2,383,111 + RR42_RS31225 0.54 +0.5 2,383,112 - RR42_RS31225 0.54 -0.5 2,383,112 - RR42_RS31225 0.54 -0.4 2,383,112 - RR42_RS31225 0.54 +0.3 2,383,112 - RR42_RS31225 0.54 +1.7 2,383,112 - RR42_RS31225 0.54 -0.4 2,383,144 + RR42_RS31225 0.61 +1.1 2,383,174 + RR42_RS31225 0.68 -0.4 2,383,174 + RR42_RS31225 0.68 -0.2 2,383,225 + RR42_RS31225 0.79 -0.9 2,383,225 + RR42_RS31225 0.79 -0.2 2,383,226 - RR42_RS31225 0.79 -0.4 2,383,319 + -3.1 2,383,320 - +1.7 2,383,320 - +0.2 2,383,421 + -0.2 2,383,631 + RR42_RS31230 0.23 -2.2 2,384,015 + RR42_RS31230 0.63 +0.8 2,384,021 + RR42_RS31230 0.64 -0.2 2,384,050 + RR42_RS31230 0.67 -0.1 2,384,050 + RR42_RS31230 0.67 -1.3 2,384,051 - RR42_RS31230 0.67 +0.3 2,384,156 + RR42_RS31230 0.78 -0.4 2,384,198 + RR42_RS31230 0.82 -1.9 2,384,198 + RR42_RS31230 0.82 +0.4 2,384,199 - RR42_RS31230 0.82 +0.7 2,384,199 - RR42_RS31230 0.82 -2.3 2,384,199 - RR42_RS31230 0.82 -0.7 2,384,199 - RR42_RS31230 0.82 +0.8 2,384,199 - RR42_RS31230 0.82 +0.5 2,384,201 + RR42_RS31230 0.83 +1.4 2,384,201 + RR42_RS31230 0.83 -0.7 2,384,201 + RR42_RS31230 0.83 -0.3 2,384,202 - RR42_RS31230 0.83 -0.9 2,384,202 - RR42_RS31230 0.83 -0.0 2,384,202 - RR42_RS31230 0.83 -0.9 2,384,255 + RR42_RS31230 0.88 +0.7 2,384,264 + RR42_RS31230 0.89 +0.2 2,384,264 + RR42_RS31230 0.89 -0.9 2,384,264 + RR42_RS31230 0.89 +1.9 2,384,284 + -1.0 2,384,432 + +0.3 2,384,432 + -0.1 2,384,433 - +0.1 2,384,497 + RR42_RS31235 0.18 +0.9 2,384,497 + RR42_RS31235 0.18 +1.1 2,384,498 - RR42_RS31235 0.18 -0.1 2,384,515 - RR42_RS31235 0.20 -0.6 2,384,629 + RR42_RS31235 0.36 -0.4 2,384,630 - RR42_RS31235 0.36 +0.6 2,384,705 - RR42_RS31235 0.46 +0.6 2,384,855 + RR42_RS31235 0.66 +0.5 2,385,077 - +1.3 2,385,077 - +0.1 2,385,101 + +0.2 2,385,102 - +0.3 2,385,102 - -1.3 2,385,102 - +0.1 2,385,251 - +0.5
Or see this region's nucleotide sequence