Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31105 and RR42_RS31110 are separated by 7 nucleotides RR42_RS31110 and RR42_RS31115 are separated by 102 nucleotides
RR42_RS31105: RR42_RS31105 - acyl-CoA dehydrogenase, at 2,358,330 to 2,359,502
_RS31105
RR42_RS31110: RR42_RS31110 - ABC transporter substrate-binding protein, at 2,359,510 to 2,360,484
_RS31110
RR42_RS31115: RR42_RS31115 - ABC transporter substrate-binding protein, at 2,360,587 to 2,361,564
_RS31115
Position (kb)
2359
2360
2361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2358.523 kb on + strand, within RR42_RS31105 at 2358.523 kb on + strand, within RR42_RS31105 at 2358.533 kb on + strand, within RR42_RS31105 at 2358.569 kb on - strand, within RR42_RS31105 at 2358.596 kb on + strand, within RR42_RS31105 at 2358.597 kb on - strand, within RR42_RS31105 at 2358.597 kb on - strand, within RR42_RS31105 at 2358.695 kb on - strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.785 kb on - strand, within RR42_RS31105 at 2358.846 kb on - strand, within RR42_RS31105 at 2358.928 kb on + strand, within RR42_RS31105 at 2358.929 kb on - strand, within RR42_RS31105 at 2358.929 kb on - strand, within RR42_RS31105 at 2358.929 kb on - strand, within RR42_RS31105 at 2359.232 kb on + strand, within RR42_RS31105 at 2359.232 kb on + strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.327 kb on + strand, within RR42_RS31105 at 2359.328 kb on - strand, within RR42_RS31105 at 2359.328 kb on - strand, within RR42_RS31105 at 2359.424 kb on - strand at 2359.446 kb on + strand at 2359.446 kb on + strand at 2359.446 kb on + strand at 2359.446 kb on + strand at 2359.447 kb on - strand at 2359.506 kb on + strand at 2359.506 kb on + strand at 2359.506 kb on + strand at 2359.741 kb on - strand, within RR42_RS31110 at 2359.741 kb on - strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.935 kb on - strand, within RR42_RS31110 at 2359.935 kb on - strand, within RR42_RS31110 at 2360.150 kb on + strand, within RR42_RS31110 at 2360.150 kb on + strand, within RR42_RS31110 at 2360.150 kb on + strand, within RR42_RS31110 at 2360.399 kb on + strand at 2360.530 kb on + strand at 2360.530 kb on + strand at 2360.530 kb on + strand at 2360.588 kb on + strand at 2360.609 kb on + strand at 2360.609 kb on + strand at 2360.610 kb on - strand at 2360.610 kb on - strand at 2360.610 kb on - strand at 2360.699 kb on + strand, within RR42_RS31115 at 2360.699 kb on + strand, within RR42_RS31115 at 2360.699 kb on + strand, within RR42_RS31115 at 2360.700 kb on - strand, within RR42_RS31115 at 2360.700 kb on - strand, within RR42_RS31115 at 2361.248 kb on + strand, within RR42_RS31115 at 2361.248 kb on + strand, within RR42_RS31115 at 2361.249 kb on - strand, within RR42_RS31115 at 2361.249 kb on - strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.298 kb on - strand, within RR42_RS31115 at 2361.298 kb on - strand, within RR42_RS31115 at 2361.482 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,358,523 + RR42_RS31105 0.16 -0.2 2,358,523 + RR42_RS31105 0.16 +0.2 2,358,533 + RR42_RS31105 0.17 -0.4 2,358,569 - RR42_RS31105 0.20 -0.6 2,358,596 + RR42_RS31105 0.23 -0.7 2,358,597 - RR42_RS31105 0.23 -2.5 2,358,597 - RR42_RS31105 0.23 +0.7 2,358,695 - RR42_RS31105 0.31 -0.3 2,358,784 + RR42_RS31105 0.39 +0.5 2,358,784 + RR42_RS31105 0.39 +0.4 2,358,784 + RR42_RS31105 0.39 +2.5 2,358,784 + RR42_RS31105 0.39 -1.1 2,358,785 - RR42_RS31105 0.39 +0.0 2,358,846 - RR42_RS31105 0.44 -0.5 2,358,928 + RR42_RS31105 0.51 -1.3 2,358,929 - RR42_RS31105 0.51 -0.8 2,358,929 - RR42_RS31105 0.51 -0.6 2,358,929 - RR42_RS31105 0.51 +0.9 2,359,232 + RR42_RS31105 0.77 +0.8 2,359,232 + RR42_RS31105 0.77 +1.1 2,359,233 - RR42_RS31105 0.77 +0.7 2,359,233 - RR42_RS31105 0.77 -0.5 2,359,233 - RR42_RS31105 0.77 -0.5 2,359,233 - RR42_RS31105 0.77 -2.9 2,359,233 - RR42_RS31105 0.77 +0.6 2,359,233 - RR42_RS31105 0.77 +0.3 2,359,233 - RR42_RS31105 0.77 +0.3 2,359,327 + RR42_RS31105 0.85 -3.0 2,359,328 - RR42_RS31105 0.85 -2.6 2,359,328 - RR42_RS31105 0.85 -1.2 2,359,424 - +0.2 2,359,446 + -1.5 2,359,446 + -1.3 2,359,446 + -0.8 2,359,446 + -1.1 2,359,447 - +0.2 2,359,506 + -1.5 2,359,506 + -1.3 2,359,506 + +0.3 2,359,741 - RR42_RS31110 0.24 +0.6 2,359,741 - RR42_RS31110 0.24 -0.3 2,359,934 + RR42_RS31110 0.43 -1.4 2,359,934 + RR42_RS31110 0.43 -0.1 2,359,934 + RR42_RS31110 0.43 -1.1 2,359,934 + RR42_RS31110 0.43 -0.4 2,359,935 - RR42_RS31110 0.44 -0.7 2,359,935 - RR42_RS31110 0.44 -1.7 2,360,150 + RR42_RS31110 0.66 -0.1 2,360,150 + RR42_RS31110 0.66 -1.3 2,360,150 + RR42_RS31110 0.66 -0.9 2,360,399 + -1.3 2,360,530 + +1.1 2,360,530 + -0.7 2,360,530 + -0.6 2,360,588 + -0.4 2,360,609 + +0.5 2,360,609 + +0.1 2,360,610 - +1.9 2,360,610 - -0.0 2,360,610 - -0.8 2,360,699 + RR42_RS31115 0.11 +0.7 2,360,699 + RR42_RS31115 0.11 +1.0 2,360,699 + RR42_RS31115 0.11 -1.1 2,360,700 - RR42_RS31115 0.12 -0.9 2,360,700 - RR42_RS31115 0.12 +0.7 2,361,248 + RR42_RS31115 0.68 -1.2 2,361,248 + RR42_RS31115 0.68 -0.1 2,361,249 - RR42_RS31115 0.68 +1.0 2,361,249 - RR42_RS31115 0.68 +1.6 2,361,297 + RR42_RS31115 0.73 +0.4 2,361,297 + RR42_RS31115 0.73 -0.3 2,361,297 + RR42_RS31115 0.73 -0.2 2,361,297 + RR42_RS31115 0.73 +0.5 2,361,298 - RR42_RS31115 0.73 +0.9 2,361,298 - RR42_RS31115 0.73 +0.4 2,361,482 + -0.9
Or see this region's nucleotide sequence