Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31075 and RR42_RS31080 are separated by 200 nucleotides RR42_RS31080 and RR42_RS31085 are separated by 297 nucleotides
RR42_RS31075: RR42_RS31075 - MFS transporter, at 2,352,786 to 2,353,814
_RS31075
RR42_RS31080: RR42_RS31080 - hypothetical protein, at 2,354,015 to 2,354,470
_RS31080
RR42_RS31085: RR42_RS31085 - hypothetical protein, at 2,354,768 to 2,355,184
_RS31085
Position (kb)
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2353.098 kb on + strand, within RR42_RS31075 at 2353.098 kb on + strand, within RR42_RS31075 at 2353.098 kb on + strand, within RR42_RS31075 at 2353.099 kb on - strand, within RR42_RS31075 at 2353.100 kb on + strand, within RR42_RS31075 at 2353.101 kb on - strand, within RR42_RS31075 at 2353.149 kb on + strand, within RR42_RS31075 at 2353.150 kb on - strand, within RR42_RS31075 at 2353.167 kb on + strand, within RR42_RS31075 at 2353.168 kb on - strand, within RR42_RS31075 at 2353.392 kb on + strand, within RR42_RS31075 at 2353.392 kb on + strand, within RR42_RS31075 at 2353.393 kb on - strand, within RR42_RS31075 at 2353.393 kb on - strand, within RR42_RS31075 at 2353.476 kb on + strand, within RR42_RS31075 at 2353.476 kb on + strand, within RR42_RS31075 at 2353.477 kb on - strand, within RR42_RS31075 at 2353.477 kb on - strand, within RR42_RS31075 at 2353.480 kb on + strand, within RR42_RS31075 at 2353.480 kb on + strand, within RR42_RS31075 at 2353.481 kb on - strand, within RR42_RS31075 at 2353.812 kb on + strand at 2353.812 kb on + strand at 2353.812 kb on + strand at 2353.812 kb on + strand at 2353.917 kb on + strand at 2353.927 kb on - strand at 2354.001 kb on + strand at 2354.001 kb on + strand at 2354.001 kb on + strand at 2354.030 kb on + strand at 2354.031 kb on - strand at 2354.031 kb on - strand at 2354.031 kb on - strand at 2354.031 kb on - strand at 2354.256 kb on - strand, within RR42_RS31080 at 2354.256 kb on - strand, within RR42_RS31080 at 2354.390 kb on + strand, within RR42_RS31080 at 2354.445 kb on - strand at 2354.764 kb on + strand at 2354.862 kb on + strand, within RR42_RS31085 at 2354.863 kb on + strand, within RR42_RS31085 at 2354.863 kb on + strand, within RR42_RS31085 at 2354.863 kb on + strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.864 kb on - strand, within RR42_RS31085 at 2354.865 kb on + strand, within RR42_RS31085 at 2354.865 kb on + strand, within RR42_RS31085 at 2354.866 kb on - strand, within RR42_RS31085 at 2354.866 kb on - strand, within RR42_RS31085 at 2354.998 kb on + strand, within RR42_RS31085 at 2354.998 kb on + strand, within RR42_RS31085 at 2354.999 kb on - strand, within RR42_RS31085 at 2354.999 kb on - strand, within RR42_RS31085 at 2355.124 kb on - strand, within RR42_RS31085 at 2355.217 kb on + strand at 2355.218 kb on - strand at 2355.242 kb on + strand at 2355.243 kb on - strand at 2355.343 kb on + strand at 2355.344 kb on - strand at 2355.348 kb on + strand at 2355.348 kb on + strand at 2355.348 kb on + strand at 2355.348 kb on + strand at 2355.349 kb on - strand at 2355.349 kb on - strand at 2355.349 kb on - strand at 2355.349 kb on - strand at 2355.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,353,098 + RR42_RS31075 0.30 +1.4 2,353,098 + RR42_RS31075 0.30 +0.1 2,353,098 + RR42_RS31075 0.30 +0.0 2,353,099 - RR42_RS31075 0.30 -0.6 2,353,100 + RR42_RS31075 0.31 -1.1 2,353,101 - RR42_RS31075 0.31 -0.4 2,353,149 + RR42_RS31075 0.35 -1.3 2,353,150 - RR42_RS31075 0.35 +0.5 2,353,167 + RR42_RS31075 0.37 +1.1 2,353,168 - RR42_RS31075 0.37 +1.1 2,353,392 + RR42_RS31075 0.59 +0.4 2,353,392 + RR42_RS31075 0.59 -1.9 2,353,393 - RR42_RS31075 0.59 -0.7 2,353,393 - RR42_RS31075 0.59 -1.6 2,353,476 + RR42_RS31075 0.67 -1.4 2,353,476 + RR42_RS31075 0.67 +0.0 2,353,477 - RR42_RS31075 0.67 +0.1 2,353,477 - RR42_RS31075 0.67 -1.6 2,353,480 + RR42_RS31075 0.67 -0.8 2,353,480 + RR42_RS31075 0.67 -1.3 2,353,481 - RR42_RS31075 0.68 -2.3 2,353,812 + -0.5 2,353,812 + -0.8 2,353,812 + -0.5 2,353,812 + +1.9 2,353,917 + +0.5 2,353,927 - +0.2 2,354,001 + -2.1 2,354,001 + -1.2 2,354,001 + -0.8 2,354,030 + -1.3 2,354,031 - -0.3 2,354,031 - -1.2 2,354,031 - -0.3 2,354,031 - -1.4 2,354,256 - RR42_RS31080 0.53 +2.9 2,354,256 - RR42_RS31080 0.53 +0.3 2,354,390 + RR42_RS31080 0.82 +0.1 2,354,445 - -1.3 2,354,764 + -0.3 2,354,862 + RR42_RS31085 0.23 +1.4 2,354,863 + RR42_RS31085 0.23 +0.7 2,354,863 + RR42_RS31085 0.23 +0.5 2,354,863 + RR42_RS31085 0.23 -2.9 2,354,864 - RR42_RS31085 0.23 -0.5 2,354,864 - RR42_RS31085 0.23 -0.4 2,354,864 - RR42_RS31085 0.23 +0.2 2,354,864 - RR42_RS31085 0.23 -0.3 2,354,864 - RR42_RS31085 0.23 -1.0 2,354,864 - RR42_RS31085 0.23 -0.3 2,354,864 - RR42_RS31085 0.23 -0.5 2,354,864 - RR42_RS31085 0.23 -1.6 2,354,865 + RR42_RS31085 0.23 -0.4 2,354,865 + RR42_RS31085 0.23 -1.1 2,354,866 - RR42_RS31085 0.24 +0.3 2,354,866 - RR42_RS31085 0.24 +0.6 2,354,998 + RR42_RS31085 0.55 +1.2 2,354,998 + RR42_RS31085 0.55 -3.2 2,354,999 - RR42_RS31085 0.55 -0.0 2,354,999 - RR42_RS31085 0.55 +0.8 2,355,124 - RR42_RS31085 0.85 -0.0 2,355,217 + -1.6 2,355,218 - -2.0 2,355,242 + +0.1 2,355,243 - -2.1 2,355,343 + -1.9 2,355,344 - -0.6 2,355,348 + -2.6 2,355,348 + +0.3 2,355,348 + -1.0 2,355,348 + -1.2 2,355,349 - -1.4 2,355,349 - +1.6 2,355,349 - +0.5 2,355,349 - +0.1 2,355,349 - +0.2
Or see this region's nucleotide sequence