Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30800 and RR42_RS30805 are separated by 106 nucleotides RR42_RS30805 and RR42_RS30810 overlap by 11 nucleotides RR42_RS30810 and RR42_RS30815 overlap by 1 nucleotides
RR42_RS30800: RR42_RS30800 - sugar ABC transporter ATP-binding protein, at 2,287,928 to 2,289,520
_RS30800
RR42_RS30805: RR42_RS30805 - ABC transporter substrate-binding protein, at 2,289,627 to 2,290,634
_RS30805
RR42_RS30810: RR42_RS30810 - TetR family transcriptional regulator, at 2,290,624 to 2,291,340
_RS30810
RR42_RS30815: RR42_RS30815 - uracil phosphoribosyltransferase, at 2,291,340 to 2,291,999
_RS30815
Position (kb)
2289
2290
2291 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2288.708 kb on + strand, within RR42_RS30800 at 2288.708 kb on + strand, within RR42_RS30800 at 2288.709 kb on - strand, within RR42_RS30800 at 2289.052 kb on - strand, within RR42_RS30800 at 2289.357 kb on + strand, within RR42_RS30800 at 2289.357 kb on + strand, within RR42_RS30800 at 2289.358 kb on - strand, within RR42_RS30800 at 2289.358 kb on - strand, within RR42_RS30800 at 2289.358 kb on - strand, within RR42_RS30800 at 2289.358 kb on - strand, within RR42_RS30800 at 2289.358 kb on - strand, within RR42_RS30800 at 2289.451 kb on + strand at 2289.451 kb on + strand at 2289.487 kb on + strand at 2289.487 kb on + strand at 2289.488 kb on - strand at 2289.508 kb on + strand at 2289.508 kb on + strand at 2289.515 kb on - strand at 2289.734 kb on + strand, within RR42_RS30805 at 2289.800 kb on - strand, within RR42_RS30805 at 2289.891 kb on - strand, within RR42_RS30805 at 2290.210 kb on + strand, within RR42_RS30805 at 2290.210 kb on + strand, within RR42_RS30805 at 2290.228 kb on + strand, within RR42_RS30805 at 2290.228 kb on + strand, within RR42_RS30805 at 2290.228 kb on + strand, within RR42_RS30805 at 2290.228 kb on + strand, within RR42_RS30805 at 2290.228 kb on + strand, within RR42_RS30805 at 2290.229 kb on - strand, within RR42_RS30805 at 2290.229 kb on - strand, within RR42_RS30805 at 2290.229 kb on - strand, within RR42_RS30805 at 2290.229 kb on - strand, within RR42_RS30805 at 2290.229 kb on - strand, within RR42_RS30805 at 2290.229 kb on - strand, within RR42_RS30805 at 2290.251 kb on - strand, within RR42_RS30805 at 2290.300 kb on + strand, within RR42_RS30805 at 2290.399 kb on + strand, within RR42_RS30805 at 2290.400 kb on - strand, within RR42_RS30805 at 2290.432 kb on + strand, within RR42_RS30805 at 2290.432 kb on + strand, within RR42_RS30805 at 2290.432 kb on + strand, within RR42_RS30805 at 2290.432 kb on + strand, within RR42_RS30805 at 2290.433 kb on - strand, within RR42_RS30805 at 2290.433 kb on - strand, within RR42_RS30805 at 2290.433 kb on - strand, within RR42_RS30805 at 2290.433 kb on - strand, within RR42_RS30805 at 2290.513 kb on + strand, within RR42_RS30805 at 2290.514 kb on - strand, within RR42_RS30805 at 2290.619 kb on + strand at 2290.619 kb on + strand at 2290.658 kb on - strand at 2290.658 kb on - strand at 2290.737 kb on - strand, within RR42_RS30810 at 2290.737 kb on - strand, within RR42_RS30810 at 2290.799 kb on + strand, within RR42_RS30810 at 2290.799 kb on + strand, within RR42_RS30810 at 2290.799 kb on + strand, within RR42_RS30810 at 2291.027 kb on + strand, within RR42_RS30810 at 2291.027 kb on + strand, within RR42_RS30810 at 2291.028 kb on - strand, within RR42_RS30810 at 2291.028 kb on - strand, within RR42_RS30810 at 2291.028 kb on - strand, within RR42_RS30810 at 2291.100 kb on + strand, within RR42_RS30810 at 2291.100 kb on + strand, within RR42_RS30810 at 2291.144 kb on + strand, within RR42_RS30810 at 2291.145 kb on - strand, within RR42_RS30810 at 2291.145 kb on - strand, within RR42_RS30810 at 2291.147 kb on + strand, within RR42_RS30810 at 2291.147 kb on + strand, within RR42_RS30810 at 2291.148 kb on - strand, within RR42_RS30810 at 2291.148 kb on - strand, within RR42_RS30810 at 2291.231 kb on + strand, within RR42_RS30810 at 2291.232 kb on - strand, within RR42_RS30810 at 2291.393 kb on - strand at 2291.393 kb on - strand at 2291.440 kb on - strand, within RR42_RS30815 at 2291.500 kb on + strand, within RR42_RS30815 at 2291.501 kb on - strand, within RR42_RS30815 at 2291.501 kb on - strand, within RR42_RS30815 at 2291.626 kb on + strand, within RR42_RS30815 at 2291.627 kb on - strand, within RR42_RS30815
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,288,708 + RR42_RS30800 0.49 -0.5 2,288,708 + RR42_RS30800 0.49 +1.8 2,288,709 - RR42_RS30800 0.49 +0.7 2,289,052 - RR42_RS30800 0.71 -0.6 2,289,357 + RR42_RS30800 0.90 +1.3 2,289,357 + RR42_RS30800 0.90 +0.6 2,289,358 - RR42_RS30800 0.90 -1.9 2,289,358 - RR42_RS30800 0.90 +0.3 2,289,358 - RR42_RS30800 0.90 +0.2 2,289,358 - RR42_RS30800 0.90 -0.3 2,289,358 - RR42_RS30800 0.90 -0.9 2,289,451 + +0.5 2,289,451 + +2.3 2,289,487 + -0.6 2,289,487 + -2.3 2,289,488 - +0.4 2,289,508 + -0.4 2,289,508 + -0.2 2,289,515 - -0.6 2,289,734 + RR42_RS30805 0.11 -0.0 2,289,800 - RR42_RS30805 0.17 -0.9 2,289,891 - RR42_RS30805 0.26 -0.1 2,290,210 + RR42_RS30805 0.58 +1.1 2,290,210 + RR42_RS30805 0.58 +0.6 2,290,228 + RR42_RS30805 0.60 -0.2 2,290,228 + RR42_RS30805 0.60 +0.3 2,290,228 + RR42_RS30805 0.60 +0.6 2,290,228 + RR42_RS30805 0.60 +0.8 2,290,228 + RR42_RS30805 0.60 +1.6 2,290,229 - RR42_RS30805 0.60 +0.1 2,290,229 - RR42_RS30805 0.60 +1.1 2,290,229 - RR42_RS30805 0.60 +0.5 2,290,229 - RR42_RS30805 0.60 +1.8 2,290,229 - RR42_RS30805 0.60 +0.9 2,290,229 - RR42_RS30805 0.60 +0.0 2,290,251 - RR42_RS30805 0.62 -0.1 2,290,300 + RR42_RS30805 0.67 +1.5 2,290,399 + RR42_RS30805 0.77 +1.7 2,290,400 - RR42_RS30805 0.77 -2.9 2,290,432 + RR42_RS30805 0.80 -0.4 2,290,432 + RR42_RS30805 0.80 -0.5 2,290,432 + RR42_RS30805 0.80 +1.0 2,290,432 + RR42_RS30805 0.80 -3.8 2,290,433 - RR42_RS30805 0.80 -0.2 2,290,433 - RR42_RS30805 0.80 +0.5 2,290,433 - RR42_RS30805 0.80 +0.2 2,290,433 - RR42_RS30805 0.80 -2.1 2,290,513 + RR42_RS30805 0.88 -0.6 2,290,514 - RR42_RS30805 0.88 +0.7 2,290,619 + +1.0 2,290,619 + +0.7 2,290,658 - +0.3 2,290,658 - +1.3 2,290,737 - RR42_RS30810 0.16 +1.4 2,290,737 - RR42_RS30810 0.16 +0.9 2,290,799 + RR42_RS30810 0.24 -2.4 2,290,799 + RR42_RS30810 0.24 +0.5 2,290,799 + RR42_RS30810 0.24 +0.4 2,291,027 + RR42_RS30810 0.56 -0.9 2,291,027 + RR42_RS30810 0.56 -1.2 2,291,028 - RR42_RS30810 0.56 +0.3 2,291,028 - RR42_RS30810 0.56 +1.1 2,291,028 - RR42_RS30810 0.56 -2.3 2,291,100 + RR42_RS30810 0.66 +1.9 2,291,100 + RR42_RS30810 0.66 -0.3 2,291,144 + RR42_RS30810 0.73 +0.2 2,291,145 - RR42_RS30810 0.73 +3.7 2,291,145 - RR42_RS30810 0.73 -0.3 2,291,147 + RR42_RS30810 0.73 -1.3 2,291,147 + RR42_RS30810 0.73 +1.5 2,291,148 - RR42_RS30810 0.73 +1.0 2,291,148 - RR42_RS30810 0.73 +0.2 2,291,231 + RR42_RS30810 0.85 -0.1 2,291,232 - RR42_RS30810 0.85 +1.0 2,291,393 - +1.7 2,291,393 - -2.1 2,291,440 - RR42_RS30815 0.15 -0.5 2,291,500 + RR42_RS30815 0.24 +0.9 2,291,501 - RR42_RS30815 0.24 +0.8 2,291,501 - RR42_RS30815 0.24 +0.7 2,291,626 + RR42_RS30815 0.43 -0.7 2,291,627 - RR42_RS30815 0.43 +0.2
Or see this region's nucleotide sequence