Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30510 and RR42_RS30515 are separated by 41 nucleotides RR42_RS30515 and RR42_RS30520 are separated by 66 nucleotides RR42_RS30520 and RR42_RS30525 are separated by 157 nucleotides
RR42_RS30510: RR42_RS30510 - maleylacetoacetate isomerase, at 2,219,305 to 2,219,949
_RS30510
RR42_RS30515: RR42_RS30515 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,219,991 to 2,220,689
_RS30515
RR42_RS30520: RR42_RS30520 - gentisate 1,2-dioxygenase, at 2,220,756 to 2,221,802
_RS30520
RR42_RS30525: RR42_RS30525 - LysR family transcriptional regulator, at 2,221,960 to 2,222,904
_RS30525
Position (kb)
2220
2221
2222 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.823 kb on - strand, within RR42_RS30510 at 2219.937 kb on + strand at 2219.938 kb on - strand at 2219.938 kb on - strand at 2219.939 kb on + strand at 2220.089 kb on - strand, within RR42_RS30515 at 2220.119 kb on - strand, within RR42_RS30515 at 2220.401 kb on + strand, within RR42_RS30515 at 2220.402 kb on - strand, within RR42_RS30515 at 2220.445 kb on + strand, within RR42_RS30515 at 2220.446 kb on - strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.589 kb on + strand, within RR42_RS30515 at 2220.590 kb on - strand, within RR42_RS30515 at 2220.674 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.739 kb on - strand at 2220.739 kb on - strand at 2220.793 kb on - strand at 2220.811 kb on + strand at 2220.946 kb on + strand, within RR42_RS30520 at 2220.946 kb on + strand, within RR42_RS30520 at 2220.946 kb on + strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.971 kb on - strand, within RR42_RS30520 at 2221.000 kb on + strand, within RR42_RS30520 at 2221.013 kb on + strand, within RR42_RS30520 at 2221.014 kb on - strand, within RR42_RS30520 at 2221.057 kb on + strand, within RR42_RS30520 at 2221.058 kb on - strand, within RR42_RS30520 at 2221.136 kb on - strand, within RR42_RS30520 at 2221.141 kb on + strand, within RR42_RS30520 at 2221.141 kb on + strand, within RR42_RS30520 at 2221.142 kb on - strand, within RR42_RS30520 at 2221.258 kb on + strand, within RR42_RS30520 at 2221.461 kb on - strand, within RR42_RS30520 at 2221.461 kb on - strand, within RR42_RS30520 at 2221.575 kb on - strand, within RR42_RS30520 at 2221.660 kb on + strand, within RR42_RS30520 at 2221.762 kb on + strand at 2221.762 kb on + strand at 2221.765 kb on + strand at 2221.765 kb on + strand at 2221.766 kb on - strand at 2221.766 kb on - strand at 2221.766 kb on - strand at 2221.877 kb on + strand at 2221.877 kb on + strand at 2221.878 kb on - strand at 2221.878 kb on - strand at 2221.879 kb on + strand at 2221.879 kb on + strand at 2221.893 kb on + strand at 2221.894 kb on - strand at 2221.932 kb on - strand at 2221.940 kb on - strand at 2222.007 kb on + strand at 2222.008 kb on - strand at 2222.464 kb on + strand, within RR42_RS30525 at 2222.464 kb on + strand, within RR42_RS30525 at 2222.465 kb on - strand, within RR42_RS30525 at 2222.465 kb on - strand, within RR42_RS30525 at 2222.465 kb on - strand, within RR42_RS30525 at 2222.465 kb on - strand, within RR42_RS30525 at 2222.465 kb on - strand, within RR42_RS30525 at 2222.465 kb on - strand, within RR42_RS30525 at 2222.549 kb on - strand, within RR42_RS30525 at 2222.549 kb on - strand, within RR42_RS30525 at 2222.691 kb on + strand, within RR42_RS30525 at 2222.691 kb on + strand, within RR42_RS30525 at 2222.692 kb on - strand, within RR42_RS30525 at 2222.692 kb on - strand, within RR42_RS30525 at 2222.692 kb on - strand, within RR42_RS30525 at 2222.703 kb on - strand, within RR42_RS30525 at 2222.794 kb on + strand, within RR42_RS30525 at 2222.794 kb on + strand, within RR42_RS30525 at 2222.795 kb on - strand, within RR42_RS30525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,219,822 + RR42_RS30510 0.80 -0.9 2,219,822 + RR42_RS30510 0.80 -2.1 2,219,822 + RR42_RS30510 0.80 -0.6 2,219,822 + RR42_RS30510 0.80 +0.1 2,219,823 - RR42_RS30510 0.80 -0.6 2,219,937 + -0.3 2,219,938 - +0.6 2,219,938 - +0.3 2,219,939 + -2.4 2,220,089 - RR42_RS30515 0.14 -2.5 2,220,119 - RR42_RS30515 0.18 +0.9 2,220,401 + RR42_RS30515 0.59 +0.1 2,220,402 - RR42_RS30515 0.59 -0.1 2,220,445 + RR42_RS30515 0.65 -0.6 2,220,446 - RR42_RS30515 0.65 +1.3 2,220,451 + RR42_RS30515 0.66 -1.6 2,220,451 + RR42_RS30515 0.66 +0.6 2,220,451 + RR42_RS30515 0.66 +0.5 2,220,452 - RR42_RS30515 0.66 -0.4 2,220,452 - RR42_RS30515 0.66 -0.3 2,220,452 - RR42_RS30515 0.66 -1.4 2,220,452 - RR42_RS30515 0.66 -0.4 2,220,589 + RR42_RS30515 0.86 -1.9 2,220,590 - RR42_RS30515 0.86 -0.3 2,220,674 + +0.5 2,220,738 + +0.1 2,220,738 + +0.2 2,220,738 + -1.4 2,220,738 + +0.4 2,220,738 + -0.3 2,220,739 - +0.8 2,220,739 - -1.4 2,220,793 - -1.1 2,220,811 + +0.6 2,220,946 + RR42_RS30520 0.18 -0.7 2,220,946 + RR42_RS30520 0.18 -0.0 2,220,946 + RR42_RS30520 0.18 +1.1 2,220,947 - RR42_RS30520 0.18 -0.2 2,220,947 - RR42_RS30520 0.18 -1.8 2,220,947 - RR42_RS30520 0.18 +0.6 2,220,947 - RR42_RS30520 0.18 -1.9 2,220,971 - RR42_RS30520 0.21 +0.0 2,221,000 + RR42_RS30520 0.23 +1.6 2,221,013 + RR42_RS30520 0.25 -0.5 2,221,014 - RR42_RS30520 0.25 +0.6 2,221,057 + RR42_RS30520 0.29 -0.0 2,221,058 - RR42_RS30520 0.29 -0.4 2,221,136 - RR42_RS30520 0.36 -1.1 2,221,141 + RR42_RS30520 0.37 +0.6 2,221,141 + RR42_RS30520 0.37 -0.3 2,221,142 - RR42_RS30520 0.37 -0.0 2,221,258 + RR42_RS30520 0.48 -0.9 2,221,461 - RR42_RS30520 0.67 -3.3 2,221,461 - RR42_RS30520 0.67 +1.0 2,221,575 - RR42_RS30520 0.78 +0.3 2,221,660 + RR42_RS30520 0.86 -0.1 2,221,762 + -0.4 2,221,762 + +1.4 2,221,765 + +1.0 2,221,765 + -0.4 2,221,766 - -1.3 2,221,766 - -2.5 2,221,766 - +0.3 2,221,877 + +0.5 2,221,877 + +1.9 2,221,878 - +1.3 2,221,878 - -0.8 2,221,879 + -0.4 2,221,879 + -0.7 2,221,893 + +0.2 2,221,894 - +0.0 2,221,932 - -2.1 2,221,940 - -1.1 2,222,007 + -0.9 2,222,008 - +0.4 2,222,464 + RR42_RS30525 0.53 +1.6 2,222,464 + RR42_RS30525 0.53 +1.5 2,222,465 - RR42_RS30525 0.53 -0.4 2,222,465 - RR42_RS30525 0.53 -0.7 2,222,465 - RR42_RS30525 0.53 +0.3 2,222,465 - RR42_RS30525 0.53 -0.1 2,222,465 - RR42_RS30525 0.53 +0.4 2,222,465 - RR42_RS30525 0.53 +0.5 2,222,549 - RR42_RS30525 0.62 +1.3 2,222,549 - RR42_RS30525 0.62 -0.0 2,222,691 + RR42_RS30525 0.77 +0.7 2,222,691 + RR42_RS30525 0.77 -2.9 2,222,692 - RR42_RS30525 0.77 -1.9 2,222,692 - RR42_RS30525 0.77 -0.9 2,222,692 - RR42_RS30525 0.77 +0.4 2,222,703 - RR42_RS30525 0.79 -0.9 2,222,794 + RR42_RS30525 0.88 +1.6 2,222,794 + RR42_RS30525 0.88 +0.6 2,222,795 - RR42_RS30525 0.88 -0.8
Or see this region's nucleotide sequence