Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30220 and RR42_RS30225 are separated by 99 nucleotides RR42_RS30225 and RR42_RS30230 are separated by 35 nucleotides
RR42_RS30220: RR42_RS30220 - porin, at 2,155,573 to 2,156,727
_RS30220
RR42_RS30225: RR42_RS30225 - allantoate amidohydrolase, at 2,156,827 to 2,158,092
_RS30225
RR42_RS30230: RR42_RS30230 - allantoinase, at 2,158,128 to 2,159,483
_RS30230
Position (kb)
2156
2157
2158
2159 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2156.080 kb on + strand, within RR42_RS30220 at 2156.080 kb on + strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.182 kb on + strand, within RR42_RS30220 at 2156.183 kb on - strand, within RR42_RS30220 at 2156.288 kb on - strand, within RR42_RS30220 at 2156.289 kb on + strand, within RR42_RS30220 at 2156.311 kb on + strand, within RR42_RS30220 at 2156.395 kb on + strand, within RR42_RS30220 at 2156.531 kb on + strand, within RR42_RS30220 at 2156.532 kb on - strand, within RR42_RS30220 at 2156.532 kb on - strand, within RR42_RS30220 at 2156.549 kb on - strand, within RR42_RS30220 at 2156.620 kb on + strand at 2156.621 kb on - strand at 2156.621 kb on - strand at 2156.870 kb on + strand at 2156.870 kb on + strand at 2156.870 kb on + strand at 2156.870 kb on + strand at 2156.985 kb on + strand, within RR42_RS30225 at 2156.985 kb on + strand, within RR42_RS30225 at 2156.986 kb on - strand, within RR42_RS30225 at 2156.987 kb on + strand, within RR42_RS30225 at 2156.988 kb on - strand, within RR42_RS30225 at 2157.117 kb on + strand, within RR42_RS30225 at 2157.117 kb on + strand, within RR42_RS30225 at 2157.118 kb on - strand, within RR42_RS30225 at 2157.296 kb on + strand, within RR42_RS30225 at 2157.592 kb on + strand, within RR42_RS30225 at 2157.640 kb on - strand, within RR42_RS30225 at 2158.083 kb on + strand at 2158.108 kb on + strand at 2158.108 kb on + strand at 2158.109 kb on - strand at 2158.192 kb on + strand at 2158.627 kb on + strand, within RR42_RS30230 at 2158.659 kb on - strand, within RR42_RS30230 at 2158.685 kb on - strand, within RR42_RS30230 at 2158.685 kb on - strand, within RR42_RS30230 at 2158.729 kb on + strand, within RR42_RS30230 at 2158.729 kb on + strand, within RR42_RS30230 at 2158.730 kb on - strand, within RR42_RS30230 at 2158.733 kb on + strand, within RR42_RS30230 at 2158.734 kb on - strand, within RR42_RS30230 at 2158.734 kb on - strand, within RR42_RS30230 at 2158.810 kb on + strand, within RR42_RS30230 at 2158.811 kb on - strand, within RR42_RS30230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,156,080 + RR42_RS30220 0.44 -1.9 2,156,080 + RR42_RS30220 0.44 +0.1 2,156,081 - RR42_RS30220 0.44 -0.0 2,156,081 - RR42_RS30220 0.44 -0.8 2,156,081 - RR42_RS30220 0.44 +0.1 2,156,081 - RR42_RS30220 0.44 +0.2 2,156,081 - RR42_RS30220 0.44 -1.1 2,156,081 - RR42_RS30220 0.44 -0.8 2,156,081 - RR42_RS30220 0.44 +1.3 2,156,081 - RR42_RS30220 0.44 +0.1 2,156,182 + RR42_RS30220 0.53 -1.5 2,156,183 - RR42_RS30220 0.53 +0.0 2,156,288 - RR42_RS30220 0.62 -0.3 2,156,289 + RR42_RS30220 0.62 -1.0 2,156,311 + RR42_RS30220 0.64 +0.2 2,156,395 + RR42_RS30220 0.71 -0.8 2,156,531 + RR42_RS30220 0.83 -1.8 2,156,532 - RR42_RS30220 0.83 +1.4 2,156,532 - RR42_RS30220 0.83 -1.7 2,156,549 - RR42_RS30220 0.85 +1.1 2,156,620 + +0.4 2,156,621 - -0.0 2,156,621 - -0.1 2,156,870 + -1.5 2,156,870 + -0.9 2,156,870 + +0.2 2,156,870 + +1.0 2,156,985 + RR42_RS30225 0.12 -1.0 2,156,985 + RR42_RS30225 0.12 -2.8 2,156,986 - RR42_RS30225 0.13 -0.2 2,156,987 + RR42_RS30225 0.13 +0.2 2,156,988 - RR42_RS30225 0.13 -2.6 2,157,117 + RR42_RS30225 0.23 +0.8 2,157,117 + RR42_RS30225 0.23 -1.7 2,157,118 - RR42_RS30225 0.23 -0.1 2,157,296 + RR42_RS30225 0.37 +0.7 2,157,592 + RR42_RS30225 0.60 -0.7 2,157,640 - RR42_RS30225 0.64 -1.7 2,158,083 + -0.3 2,158,108 + -2.0 2,158,108 + +0.1 2,158,109 - +0.4 2,158,192 + +1.9 2,158,627 + RR42_RS30230 0.37 -0.6 2,158,659 - RR42_RS30230 0.39 -1.9 2,158,685 - RR42_RS30230 0.41 +0.9 2,158,685 - RR42_RS30230 0.41 -0.8 2,158,729 + RR42_RS30230 0.44 +0.0 2,158,729 + RR42_RS30230 0.44 -0.6 2,158,730 - RR42_RS30230 0.44 -1.2 2,158,733 + RR42_RS30230 0.45 +0.5 2,158,734 - RR42_RS30230 0.45 +1.9 2,158,734 - RR42_RS30230 0.45 -0.9 2,158,810 + RR42_RS30230 0.50 +1.1 2,158,811 - RR42_RS30230 0.50 -1.6
Or see this region's nucleotide sequence