Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30030 and RR42_RS30035 overlap by 4 nucleotides RR42_RS30035 and RR42_RS30040 overlap by 20 nucleotides
RR42_RS30030: RR42_RS30030 - hypothetical protein, at 2,109,965 to 2,110,213
_RS30030
RR42_RS30035: RR42_RS30035 - hemolysin D, at 2,110,210 to 2,111,259
_RS30035
RR42_RS30040: RR42_RS30040 - fusaric acid resistance protein, at 2,111,240 to 2,113,264
_RS30040
Position (kb)
2110
2111
2112 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2109.267 kb on + strand at 2109.268 kb on - strand at 2109.301 kb on + strand at 2109.511 kb on - strand at 2109.615 kb on - strand at 2109.615 kb on - strand at 2109.662 kb on + strand at 2109.675 kb on + strand at 2109.750 kb on - strand at 2109.799 kb on - strand at 2109.845 kb on + strand at 2109.845 kb on + strand at 2109.845 kb on + strand at 2109.846 kb on - strand at 2109.917 kb on + strand at 2109.918 kb on - strand at 2109.918 kb on - strand at 2109.918 kb on - strand at 2109.919 kb on + strand at 2109.920 kb on - strand at 2110.102 kb on + strand, within RR42_RS30030 at 2110.102 kb on + strand, within RR42_RS30030 at 2110.102 kb on + strand, within RR42_RS30030 at 2110.103 kb on - strand, within RR42_RS30030 at 2110.603 kb on + strand, within RR42_RS30035 at 2110.603 kb on + strand, within RR42_RS30035 at 2110.604 kb on - strand, within RR42_RS30035 at 2110.657 kb on + strand, within RR42_RS30035 at 2110.657 kb on + strand, within RR42_RS30035 at 2110.658 kb on - strand, within RR42_RS30035 at 2110.931 kb on + strand, within RR42_RS30035 at 2110.932 kb on - strand, within RR42_RS30035 at 2111.017 kb on + strand, within RR42_RS30035 at 2111.018 kb on - strand, within RR42_RS30035 at 2111.070 kb on + strand, within RR42_RS30035 at 2111.070 kb on + strand, within RR42_RS30035 at 2111.071 kb on - strand, within RR42_RS30035 at 2111.119 kb on + strand, within RR42_RS30035 at 2111.119 kb on + strand, within RR42_RS30035 at 2111.120 kb on - strand, within RR42_RS30035 at 2111.120 kb on - strand, within RR42_RS30035 at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.414 kb on + strand at 2111.414 kb on + strand at 2111.414 kb on + strand at 2111.415 kb on - strand at 2111.417 kb on - strand at 2111.417 kb on - strand at 2111.708 kb on + strand, within RR42_RS30040 at 2111.981 kb on - strand, within RR42_RS30040 at 2111.981 kb on - strand, within RR42_RS30040 at 2111.981 kb on - strand, within RR42_RS30040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,109,267 + +0.2 2,109,268 - -1.2 2,109,301 + +0.7 2,109,511 - +1.5 2,109,615 - -1.4 2,109,615 - -4.3 2,109,662 + -1.1 2,109,675 + -0.3 2,109,750 - +0.5 2,109,799 - -0.6 2,109,845 + +0.4 2,109,845 + -0.1 2,109,845 + -0.9 2,109,846 - -1.2 2,109,917 + +0.5 2,109,918 - +2.2 2,109,918 - +1.3 2,109,918 - -1.4 2,109,919 + -0.2 2,109,920 - +0.2 2,110,102 + RR42_RS30030 0.55 -0.1 2,110,102 + RR42_RS30030 0.55 +0.4 2,110,102 + RR42_RS30030 0.55 +0.5 2,110,103 - RR42_RS30030 0.55 +0.2 2,110,603 + RR42_RS30035 0.37 -1.3 2,110,603 + RR42_RS30035 0.37 -1.1 2,110,604 - RR42_RS30035 0.38 -2.0 2,110,657 + RR42_RS30035 0.43 -2.2 2,110,657 + RR42_RS30035 0.43 +0.8 2,110,658 - RR42_RS30035 0.43 -0.4 2,110,931 + RR42_RS30035 0.69 +0.6 2,110,932 - RR42_RS30035 0.69 +1.6 2,111,017 + RR42_RS30035 0.77 -0.6 2,111,018 - RR42_RS30035 0.77 +0.4 2,111,070 + RR42_RS30035 0.82 +0.1 2,111,070 + RR42_RS30035 0.82 +1.1 2,111,071 - RR42_RS30035 0.82 +1.0 2,111,119 + RR42_RS30035 0.87 -2.7 2,111,119 + RR42_RS30035 0.87 +0.7 2,111,120 - RR42_RS30035 0.87 -0.3 2,111,120 - RR42_RS30035 0.87 -1.1 2,111,385 - -1.5 2,111,385 - +0.7 2,111,385 - +0.3 2,111,385 - +1.0 2,111,385 - +1.5 2,111,385 - +1.3 2,111,414 + +0.0 2,111,414 + +1.1 2,111,414 + +1.5 2,111,415 - +0.6 2,111,417 - -0.6 2,111,417 - -0.5 2,111,708 + RR42_RS30040 0.23 +0.6 2,111,981 - RR42_RS30040 0.37 -1.8 2,111,981 - RR42_RS30040 0.37 -1.4 2,111,981 - RR42_RS30040 0.37 -1.7
Or see this region's nucleotide sequence