Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29955 and RR42_RS29960 overlap by 1 nucleotides RR42_RS29960 and RR42_RS29965 are separated by 402 nucleotides RR42_RS29965 and RR42_RS29970 are separated by 94 nucleotides
RR42_RS29955: RR42_RS29955 - hypothetical protein, at 2,087,042 to 2,088,457
_RS29955
RR42_RS29960: RR42_RS29960 - hypothetical protein, at 2,088,457 to 2,088,948
_RS29960
RR42_RS29965: RR42_RS29965 - LacI family transcriptional regulator, at 2,089,351 to 2,090,334
_RS29965
RR42_RS29970: RR42_RS29970 - IclR family transcriptional regulator, at 2,090,429 to 2,091,076
_RS29970
Position (kb)
2089
2090
2091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2088.370 kb on + strand at 2088.370 kb on + strand at 2088.383 kb on + strand at 2088.384 kb on - strand at 2088.384 kb on - strand at 2088.432 kb on + strand at 2088.432 kb on + strand at 2088.433 kb on - strand at 2088.533 kb on - strand, within RR42_RS29960 at 2088.541 kb on + strand, within RR42_RS29960 at 2088.541 kb on + strand, within RR42_RS29960 at 2088.542 kb on - strand, within RR42_RS29960 at 2088.542 kb on - strand, within RR42_RS29960 at 2088.649 kb on + strand, within RR42_RS29960 at 2088.650 kb on - strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.678 kb on - strand, within RR42_RS29960 at 2088.678 kb on - strand, within RR42_RS29960 at 2088.686 kb on + strand, within RR42_RS29960 at 2088.687 kb on - strand, within RR42_RS29960 at 2088.687 kb on - strand, within RR42_RS29960 at 2088.687 kb on - strand, within RR42_RS29960 at 2088.829 kb on + strand, within RR42_RS29960 at 2088.830 kb on - strand, within RR42_RS29960 at 2088.866 kb on + strand, within RR42_RS29960 at 2089.007 kb on + strand at 2089.007 kb on + strand at 2089.077 kb on + strand at 2089.077 kb on + strand at 2089.077 kb on + strand at 2089.078 kb on - strand at 2089.078 kb on - strand at 2089.119 kb on + strand at 2089.119 kb on + strand at 2089.120 kb on - strand at 2089.120 kb on - strand at 2089.135 kb on + strand at 2089.136 kb on - strand at 2089.186 kb on + strand at 2089.187 kb on - strand at 2089.187 kb on - strand at 2089.238 kb on + strand at 2089.252 kb on + strand at 2089.252 kb on + strand at 2089.252 kb on + strand at 2089.252 kb on + strand at 2089.253 kb on - strand at 2089.262 kb on + strand at 2089.262 kb on + strand at 2089.262 kb on + strand at 2089.263 kb on - strand at 2089.352 kb on + strand at 2089.352 kb on + strand at 2089.352 kb on + strand at 2089.353 kb on - strand at 2089.374 kb on + strand at 2089.375 kb on - strand at 2089.498 kb on + strand, within RR42_RS29965 at 2089.498 kb on + strand, within RR42_RS29965 at 2089.556 kb on - strand, within RR42_RS29965 at 2089.586 kb on + strand, within RR42_RS29965 at 2089.587 kb on - strand, within RR42_RS29965 at 2089.684 kb on + strand, within RR42_RS29965 at 2089.684 kb on + strand, within RR42_RS29965 at 2089.727 kb on - strand, within RR42_RS29965 at 2089.727 kb on - strand, within RR42_RS29965 at 2089.727 kb on - strand, within RR42_RS29965 at 2089.859 kb on + strand, within RR42_RS29965 at 2090.014 kb on - strand, within RR42_RS29965 at 2090.034 kb on + strand, within RR42_RS29965 at 2090.034 kb on + strand, within RR42_RS29965 at 2090.034 kb on + strand, within RR42_RS29965 at 2090.034 kb on + strand, within RR42_RS29965 at 2090.043 kb on + strand, within RR42_RS29965 at 2090.043 kb on + strand, within RR42_RS29965 at 2090.043 kb on + strand, within RR42_RS29965 at 2090.044 kb on - strand, within RR42_RS29965 at 2090.052 kb on + strand, within RR42_RS29965 at 2090.052 kb on + strand, within RR42_RS29965 at 2090.063 kb on + strand, within RR42_RS29965 at 2090.063 kb on + strand, within RR42_RS29965 at 2090.063 kb on + strand, within RR42_RS29965 at 2090.063 kb on + strand, within RR42_RS29965 at 2090.063 kb on + strand, within RR42_RS29965 at 2090.064 kb on - strand, within RR42_RS29965 at 2090.090 kb on + strand, within RR42_RS29965 at 2090.090 kb on + strand, within RR42_RS29965 at 2090.090 kb on + strand, within RR42_RS29965 at 2090.090 kb on + strand, within RR42_RS29965 at 2090.091 kb on - strand, within RR42_RS29965 at 2090.091 kb on - strand, within RR42_RS29965 at 2090.091 kb on - strand, within RR42_RS29965 at 2090.091 kb on - strand, within RR42_RS29965 at 2090.178 kb on + strand, within RR42_RS29965 at 2090.178 kb on + strand, within RR42_RS29965 at 2090.178 kb on + strand, within RR42_RS29965 at 2090.179 kb on - strand, within RR42_RS29965 at 2090.311 kb on - strand at 2090.321 kb on - strand at 2090.437 kb on + strand at 2090.437 kb on + strand at 2090.437 kb on + strand at 2090.437 kb on + strand at 2090.438 kb on - strand at 2090.438 kb on - strand at 2090.438 kb on - strand at 2090.438 kb on - strand at 2090.438 kb on - strand at 2090.438 kb on - strand at 2090.438 kb on - strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.440 kb on + strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.441 kb on - strand at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.497 kb on + strand, within RR42_RS29970 at 2090.498 kb on - strand, within RR42_RS29970 at 2090.523 kb on + strand, within RR42_RS29970 at 2090.523 kb on + strand, within RR42_RS29970 at 2090.523 kb on + strand, within RR42_RS29970 at 2090.556 kb on + strand, within RR42_RS29970 at 2090.579 kb on + strand, within RR42_RS29970 at 2090.579 kb on + strand, within RR42_RS29970 at 2090.706 kb on - strand, within RR42_RS29970 at 2090.755 kb on + strand, within RR42_RS29970 at 2090.837 kb on - strand, within RR42_RS29970 at 2090.837 kb on - strand, within RR42_RS29970 at 2091.211 kb on + strand at 2091.212 kb on - strand at 2091.212 kb on - strand at 2091.301 kb on + strand at 2091.301 kb on + strand at 2091.301 kb on + strand at 2091.302 kb on - strand at 2091.302 kb on - strand at 2091.302 kb on - strand at 2091.302 kb on - strand at 2091.327 kb on + strand at 2091.333 kb on - strand at 2091.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,088,370 + -1.6 2,088,370 + -2.4 2,088,383 + -0.3 2,088,384 - -1.7 2,088,384 - +0.6 2,088,432 + -0.3 2,088,432 + -0.3 2,088,433 - -3.3 2,088,533 - RR42_RS29960 0.15 +0.6 2,088,541 + RR42_RS29960 0.17 -0.4 2,088,541 + RR42_RS29960 0.17 -1.4 2,088,542 - RR42_RS29960 0.17 -0.2 2,088,542 - RR42_RS29960 0.17 -1.4 2,088,649 + RR42_RS29960 0.39 +1.3 2,088,650 - RR42_RS29960 0.39 -0.2 2,088,677 + RR42_RS29960 0.45 +1.8 2,088,677 + RR42_RS29960 0.45 -0.5 2,088,677 + RR42_RS29960 0.45 +0.6 2,088,677 + RR42_RS29960 0.45 -0.8 2,088,677 + RR42_RS29960 0.45 +0.2 2,088,678 - RR42_RS29960 0.45 +0.3 2,088,678 - RR42_RS29960 0.45 -0.1 2,088,686 + RR42_RS29960 0.47 -1.0 2,088,687 - RR42_RS29960 0.47 -0.2 2,088,687 - RR42_RS29960 0.47 -2.2 2,088,687 - RR42_RS29960 0.47 +0.1 2,088,829 + RR42_RS29960 0.76 -1.4 2,088,830 - RR42_RS29960 0.76 -2.0 2,088,866 + RR42_RS29960 0.83 -0.0 2,089,007 + -2.2 2,089,007 + +1.9 2,089,077 + +0.6 2,089,077 + -0.9 2,089,077 + -1.1 2,089,078 - +0.0 2,089,078 - -1.5 2,089,119 + +1.2 2,089,119 + +0.3 2,089,120 - +0.7 2,089,120 - -0.5 2,089,135 + -0.2 2,089,136 - +0.1 2,089,186 + -1.2 2,089,187 - +0.2 2,089,187 - -0.2 2,089,238 + -1.6 2,089,252 + +0.7 2,089,252 + +0.6 2,089,252 + -1.1 2,089,252 + +2.2 2,089,253 - -0.1 2,089,262 + -0.7 2,089,262 + -0.7 2,089,262 + +0.5 2,089,263 - +1.3 2,089,352 + +1.6 2,089,352 + -0.2 2,089,352 + -0.4 2,089,353 - +0.6 2,089,374 + -2.7 2,089,375 - +0.3 2,089,498 + RR42_RS29965 0.15 -0.9 2,089,498 + RR42_RS29965 0.15 -0.4 2,089,556 - RR42_RS29965 0.21 +0.2 2,089,586 + RR42_RS29965 0.24 -0.3 2,089,587 - RR42_RS29965 0.24 -2.0 2,089,684 + RR42_RS29965 0.34 -0.6 2,089,684 + RR42_RS29965 0.34 +0.7 2,089,727 - RR42_RS29965 0.38 -0.7 2,089,727 - RR42_RS29965 0.38 +0.6 2,089,727 - RR42_RS29965 0.38 -1.4 2,089,859 + RR42_RS29965 0.52 +0.8 2,090,014 - RR42_RS29965 0.67 +0.0 2,090,034 + RR42_RS29965 0.69 +0.2 2,090,034 + RR42_RS29965 0.69 -0.3 2,090,034 + RR42_RS29965 0.69 -0.4 2,090,034 + RR42_RS29965 0.69 -0.4 2,090,043 + RR42_RS29965 0.70 +0.9 2,090,043 + RR42_RS29965 0.70 -0.1 2,090,043 + RR42_RS29965 0.70 +0.0 2,090,044 - RR42_RS29965 0.70 +0.9 2,090,052 + RR42_RS29965 0.71 -0.8 2,090,052 + RR42_RS29965 0.71 +2.2 2,090,063 + RR42_RS29965 0.72 -2.9 2,090,063 + RR42_RS29965 0.72 -2.9 2,090,063 + RR42_RS29965 0.72 +0.8 2,090,063 + RR42_RS29965 0.72 -0.6 2,090,063 + RR42_RS29965 0.72 -0.4 2,090,064 - RR42_RS29965 0.72 +2.1 2,090,090 + RR42_RS29965 0.75 -1.1 2,090,090 + RR42_RS29965 0.75 -0.4 2,090,090 + RR42_RS29965 0.75 -0.7 2,090,090 + RR42_RS29965 0.75 -0.1 2,090,091 - RR42_RS29965 0.75 +0.5 2,090,091 - RR42_RS29965 0.75 +1.3 2,090,091 - RR42_RS29965 0.75 +0.8 2,090,091 - RR42_RS29965 0.75 -1.0 2,090,178 + RR42_RS29965 0.84 -1.4 2,090,178 + RR42_RS29965 0.84 -2.2 2,090,178 + RR42_RS29965 0.84 -0.0 2,090,179 - RR42_RS29965 0.84 -0.3 2,090,311 - -0.3 2,090,321 - -0.3 2,090,437 + -1.7 2,090,437 + -0.6 2,090,437 + -1.3 2,090,437 + -1.4 2,090,438 - +1.1 2,090,438 - +0.6 2,090,438 - +0.7 2,090,438 - +0.1 2,090,438 - -1.4 2,090,438 - -0.1 2,090,438 - -0.3 2,090,440 + -1.7 2,090,440 + +0.6 2,090,440 + +1.0 2,090,440 + -0.3 2,090,440 + -1.9 2,090,440 + -1.8 2,090,440 + -0.6 2,090,440 + -3.0 2,090,440 + +0.3 2,090,440 + +1.0 2,090,441 - +1.1 2,090,441 - -2.4 2,090,441 - +1.0 2,090,441 - +0.7 2,090,441 - -1.0 2,090,441 - +0.3 2,090,441 - +1.6 2,090,441 - +0.3 2,090,497 + RR42_RS29970 0.10 +0.8 2,090,497 + RR42_RS29970 0.10 -3.0 2,090,497 + RR42_RS29970 0.10 -3.5 2,090,497 + RR42_RS29970 0.10 +0.6 2,090,497 + RR42_RS29970 0.10 +0.2 2,090,497 + RR42_RS29970 0.10 +0.8 2,090,497 + RR42_RS29970 0.10 -0.6 2,090,497 + RR42_RS29970 0.10 -1.2 2,090,497 + RR42_RS29970 0.10 -0.4 2,090,497 + RR42_RS29970 0.10 -1.4 2,090,497 + RR42_RS29970 0.10 -1.8 2,090,497 + RR42_RS29970 0.10 -0.3 2,090,498 - RR42_RS29970 0.11 -2.0 2,090,523 + RR42_RS29970 0.15 -1.6 2,090,523 + RR42_RS29970 0.15 +0.3 2,090,523 + RR42_RS29970 0.15 -1.1 2,090,556 + RR42_RS29970 0.20 +1.4 2,090,579 + RR42_RS29970 0.23 -1.2 2,090,579 + RR42_RS29970 0.23 -0.5 2,090,706 - RR42_RS29970 0.43 +0.6 2,090,755 + RR42_RS29970 0.50 -0.2 2,090,837 - RR42_RS29970 0.63 -2.2 2,090,837 - RR42_RS29970 0.63 -0.4 2,091,211 + -0.3 2,091,212 - -0.4 2,091,212 - +0.7 2,091,301 + -0.1 2,091,301 + -1.0 2,091,301 + +0.3 2,091,302 - -0.4 2,091,302 - -0.6 2,091,302 - +1.0 2,091,302 - -0.2 2,091,327 + -2.0 2,091,333 - -0.9 2,091,333 - -1.7
Or see this region's nucleotide sequence