Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29950 and RR42_RS29955 are separated by 13 nucleotides RR42_RS29955 and RR42_RS29960 overlap by 1 nucleotides RR42_RS29960 and RR42_RS29965 are separated by 402 nucleotides
RR42_RS29950: RR42_RS29950 - CoA-transferase, at 2,085,760 to 2,087,028
_RS29950
RR42_RS29955: RR42_RS29955 - hypothetical protein, at 2,087,042 to 2,088,457
_RS29955
RR42_RS29960: RR42_RS29960 - hypothetical protein, at 2,088,457 to 2,088,948
_RS29960
RR42_RS29965: RR42_RS29965 - LacI family transcriptional regulator, at 2,089,351 to 2,090,334
_RS29965
Position (kb)
2087
2088
2089 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2086.042 kb on - strand, within RR42_RS29950 at 2086.042 kb on - strand, within RR42_RS29950 at 2086.042 kb on - strand, within RR42_RS29950 at 2086.047 kb on + strand, within RR42_RS29950 at 2086.047 kb on + strand, within RR42_RS29950 at 2086.173 kb on - strand, within RR42_RS29950 at 2086.255 kb on + strand, within RR42_RS29950 at 2086.255 kb on + strand, within RR42_RS29950 at 2086.256 kb on - strand, within RR42_RS29950 at 2086.256 kb on - strand, within RR42_RS29950 at 2086.263 kb on + strand, within RR42_RS29950 at 2086.264 kb on - strand, within RR42_RS29950 at 2086.289 kb on + strand, within RR42_RS29950 at 2086.469 kb on + strand, within RR42_RS29950 at 2086.469 kb on + strand, within RR42_RS29950 at 2086.469 kb on + strand, within RR42_RS29950 at 2086.470 kb on - strand, within RR42_RS29950 at 2086.470 kb on - strand, within RR42_RS29950 at 2086.549 kb on + strand, within RR42_RS29950 at 2086.589 kb on + strand, within RR42_RS29950 at 2086.608 kb on - strand, within RR42_RS29950 at 2086.635 kb on + strand, within RR42_RS29950 at 2086.636 kb on - strand, within RR42_RS29950 at 2086.636 kb on - strand, within RR42_RS29950 at 2086.643 kb on + strand, within RR42_RS29950 at 2086.644 kb on - strand, within RR42_RS29950 at 2086.644 kb on - strand, within RR42_RS29950 at 2086.680 kb on - strand, within RR42_RS29950 at 2086.680 kb on - strand, within RR42_RS29950 at 2086.680 kb on - strand, within RR42_RS29950 at 2086.714 kb on - strand, within RR42_RS29950 at 2086.723 kb on + strand, within RR42_RS29950 at 2086.723 kb on + strand, within RR42_RS29950 at 2086.723 kb on + strand, within RR42_RS29950 at 2086.724 kb on - strand, within RR42_RS29950 at 2086.829 kb on + strand, within RR42_RS29950 at 2086.893 kb on + strand, within RR42_RS29950 at 2086.893 kb on + strand, within RR42_RS29950 at 2086.894 kb on - strand, within RR42_RS29950 at 2086.959 kb on + strand at 2086.990 kb on - strand at 2087.012 kb on - strand at 2087.028 kb on + strand at 2087.071 kb on - strand at 2087.200 kb on + strand, within RR42_RS29955 at 2087.253 kb on + strand, within RR42_RS29955 at 2087.254 kb on - strand, within RR42_RS29955 at 2087.254 kb on - strand, within RR42_RS29955 at 2087.346 kb on + strand, within RR42_RS29955 at 2087.346 kb on + strand, within RR42_RS29955 at 2087.347 kb on - strand, within RR42_RS29955 at 2087.347 kb on - strand, within RR42_RS29955 at 2087.347 kb on - strand, within RR42_RS29955 at 2087.347 kb on - strand, within RR42_RS29955 at 2087.347 kb on - strand, within RR42_RS29955 at 2087.350 kb on + strand, within RR42_RS29955 at 2087.350 kb on + strand, within RR42_RS29955 at 2087.350 kb on + strand, within RR42_RS29955 at 2087.351 kb on - strand, within RR42_RS29955 at 2087.351 kb on - strand, within RR42_RS29955 at 2087.351 kb on - strand, within RR42_RS29955 at 2087.351 kb on - strand, within RR42_RS29955 at 2087.351 kb on - strand, within RR42_RS29955 at 2087.456 kb on + strand, within RR42_RS29955 at 2087.694 kb on - strand, within RR42_RS29955 at 2087.694 kb on - strand, within RR42_RS29955 at 2087.737 kb on + strand, within RR42_RS29955 at 2087.738 kb on - strand, within RR42_RS29955 at 2087.797 kb on + strand, within RR42_RS29955 at 2087.798 kb on - strand, within RR42_RS29955 at 2087.911 kb on + strand, within RR42_RS29955 at 2087.912 kb on - strand, within RR42_RS29955 at 2088.028 kb on + strand, within RR42_RS29955 at 2088.028 kb on + strand, within RR42_RS29955 at 2088.029 kb on - strand, within RR42_RS29955 at 2088.029 kb on - strand, within RR42_RS29955 at 2088.193 kb on + strand, within RR42_RS29955 at 2088.193 kb on + strand, within RR42_RS29955 at 2088.194 kb on - strand, within RR42_RS29955 at 2088.337 kb on + strand at 2088.370 kb on + strand at 2088.370 kb on + strand at 2088.383 kb on + strand at 2088.384 kb on - strand at 2088.384 kb on - strand at 2088.432 kb on + strand at 2088.432 kb on + strand at 2088.433 kb on - strand at 2088.533 kb on - strand, within RR42_RS29960 at 2088.541 kb on + strand, within RR42_RS29960 at 2088.541 kb on + strand, within RR42_RS29960 at 2088.542 kb on - strand, within RR42_RS29960 at 2088.542 kb on - strand, within RR42_RS29960 at 2088.649 kb on + strand, within RR42_RS29960 at 2088.650 kb on - strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.677 kb on + strand, within RR42_RS29960 at 2088.678 kb on - strand, within RR42_RS29960 at 2088.678 kb on - strand, within RR42_RS29960 at 2088.686 kb on + strand, within RR42_RS29960 at 2088.687 kb on - strand, within RR42_RS29960 at 2088.687 kb on - strand, within RR42_RS29960 at 2088.687 kb on - strand, within RR42_RS29960 at 2088.829 kb on + strand, within RR42_RS29960 at 2088.830 kb on - strand, within RR42_RS29960 at 2088.866 kb on + strand, within RR42_RS29960 at 2089.007 kb on + strand at 2089.007 kb on + strand at 2089.077 kb on + strand at 2089.077 kb on + strand at 2089.077 kb on + strand at 2089.078 kb on - strand at 2089.078 kb on - strand at 2089.119 kb on + strand at 2089.119 kb on + strand at 2089.120 kb on - strand at 2089.120 kb on - strand at 2089.135 kb on + strand at 2089.136 kb on - strand at 2089.186 kb on + strand at 2089.187 kb on - strand at 2089.187 kb on - strand at 2089.238 kb on + strand at 2089.252 kb on + strand at 2089.252 kb on + strand at 2089.252 kb on + strand at 2089.252 kb on + strand at 2089.253 kb on - strand at 2089.262 kb on + strand at 2089.262 kb on + strand at 2089.262 kb on + strand at 2089.263 kb on - strand at 2089.352 kb on + strand at 2089.352 kb on + strand at 2089.352 kb on + strand at 2089.353 kb on - strand at 2089.374 kb on + strand at 2089.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,086,042 - RR42_RS29950 0.22 +0.1 2,086,042 - RR42_RS29950 0.22 +0.4 2,086,042 - RR42_RS29950 0.22 +1.8 2,086,047 + RR42_RS29950 0.23 +0.5 2,086,047 + RR42_RS29950 0.23 -2.9 2,086,173 - RR42_RS29950 0.33 -1.9 2,086,255 + RR42_RS29950 0.39 +0.3 2,086,255 + RR42_RS29950 0.39 +0.2 2,086,256 - RR42_RS29950 0.39 +0.8 2,086,256 - RR42_RS29950 0.39 +1.1 2,086,263 + RR42_RS29950 0.40 +0.2 2,086,264 - RR42_RS29950 0.40 -2.0 2,086,289 + RR42_RS29950 0.42 -0.5 2,086,469 + RR42_RS29950 0.56 -1.6 2,086,469 + RR42_RS29950 0.56 -0.1 2,086,469 + RR42_RS29950 0.56 +1.5 2,086,470 - RR42_RS29950 0.56 -1.9 2,086,470 - RR42_RS29950 0.56 +0.4 2,086,549 + RR42_RS29950 0.62 -0.3 2,086,589 + RR42_RS29950 0.65 +0.9 2,086,608 - RR42_RS29950 0.67 +1.0 2,086,635 + RR42_RS29950 0.69 +0.0 2,086,636 - RR42_RS29950 0.69 +0.0 2,086,636 - RR42_RS29950 0.69 +0.7 2,086,643 + RR42_RS29950 0.70 +0.1 2,086,644 - RR42_RS29950 0.70 +1.5 2,086,644 - RR42_RS29950 0.70 +0.6 2,086,680 - RR42_RS29950 0.72 +1.9 2,086,680 - RR42_RS29950 0.72 -0.8 2,086,680 - RR42_RS29950 0.72 +1.3 2,086,714 - RR42_RS29950 0.75 -0.4 2,086,723 + RR42_RS29950 0.76 -2.2 2,086,723 + RR42_RS29950 0.76 -0.0 2,086,723 + RR42_RS29950 0.76 -0.1 2,086,724 - RR42_RS29950 0.76 -1.4 2,086,829 + RR42_RS29950 0.84 +0.9 2,086,893 + RR42_RS29950 0.89 -0.7 2,086,893 + RR42_RS29950 0.89 +0.4 2,086,894 - RR42_RS29950 0.89 +0.1 2,086,959 + -0.8 2,086,990 - +1.5 2,087,012 - -0.4 2,087,028 + +0.8 2,087,071 - +0.2 2,087,200 + RR42_RS29955 0.11 -0.1 2,087,253 + RR42_RS29955 0.15 -0.8 2,087,254 - RR42_RS29955 0.15 +0.3 2,087,254 - RR42_RS29955 0.15 -0.6 2,087,346 + RR42_RS29955 0.21 +0.7 2,087,346 + RR42_RS29955 0.21 +0.1 2,087,347 - RR42_RS29955 0.22 +0.2 2,087,347 - RR42_RS29955 0.22 -0.7 2,087,347 - RR42_RS29955 0.22 +1.1 2,087,347 - RR42_RS29955 0.22 +1.0 2,087,347 - RR42_RS29955 0.22 +0.7 2,087,350 + RR42_RS29955 0.22 -0.4 2,087,350 + RR42_RS29955 0.22 -0.6 2,087,350 + RR42_RS29955 0.22 +0.4 2,087,351 - RR42_RS29955 0.22 -0.7 2,087,351 - RR42_RS29955 0.22 +0.2 2,087,351 - RR42_RS29955 0.22 +0.1 2,087,351 - RR42_RS29955 0.22 -3.1 2,087,351 - RR42_RS29955 0.22 +1.0 2,087,456 + RR42_RS29955 0.29 -1.0 2,087,694 - RR42_RS29955 0.46 -1.0 2,087,694 - RR42_RS29955 0.46 -0.5 2,087,737 + RR42_RS29955 0.49 +1.7 2,087,738 - RR42_RS29955 0.49 -0.9 2,087,797 + RR42_RS29955 0.53 -0.4 2,087,798 - RR42_RS29955 0.53 +0.0 2,087,911 + RR42_RS29955 0.61 +1.4 2,087,912 - RR42_RS29955 0.61 -0.5 2,088,028 + RR42_RS29955 0.70 +0.5 2,088,028 + RR42_RS29955 0.70 +0.2 2,088,029 - RR42_RS29955 0.70 -0.7 2,088,029 - RR42_RS29955 0.70 -2.9 2,088,193 + RR42_RS29955 0.81 +0.3 2,088,193 + RR42_RS29955 0.81 -0.3 2,088,194 - RR42_RS29955 0.81 -0.7 2,088,337 + +1.6 2,088,370 + -1.6 2,088,370 + -2.4 2,088,383 + -0.3 2,088,384 - -1.7 2,088,384 - +0.6 2,088,432 + -0.3 2,088,432 + -0.3 2,088,433 - -3.3 2,088,533 - RR42_RS29960 0.15 +0.6 2,088,541 + RR42_RS29960 0.17 -0.4 2,088,541 + RR42_RS29960 0.17 -1.4 2,088,542 - RR42_RS29960 0.17 -0.2 2,088,542 - RR42_RS29960 0.17 -1.4 2,088,649 + RR42_RS29960 0.39 +1.3 2,088,650 - RR42_RS29960 0.39 -0.2 2,088,677 + RR42_RS29960 0.45 +1.8 2,088,677 + RR42_RS29960 0.45 -0.5 2,088,677 + RR42_RS29960 0.45 +0.6 2,088,677 + RR42_RS29960 0.45 -0.8 2,088,677 + RR42_RS29960 0.45 +0.2 2,088,678 - RR42_RS29960 0.45 +0.3 2,088,678 - RR42_RS29960 0.45 -0.1 2,088,686 + RR42_RS29960 0.47 -1.0 2,088,687 - RR42_RS29960 0.47 -0.2 2,088,687 - RR42_RS29960 0.47 -2.2 2,088,687 - RR42_RS29960 0.47 +0.1 2,088,829 + RR42_RS29960 0.76 -1.4 2,088,830 - RR42_RS29960 0.76 -2.0 2,088,866 + RR42_RS29960 0.83 -0.0 2,089,007 + -2.2 2,089,007 + +1.9 2,089,077 + +0.6 2,089,077 + -0.9 2,089,077 + -1.1 2,089,078 - +0.0 2,089,078 - -1.5 2,089,119 + +1.2 2,089,119 + +0.3 2,089,120 - +0.7 2,089,120 - -0.5 2,089,135 + -0.2 2,089,136 - +0.1 2,089,186 + -1.2 2,089,187 - +0.2 2,089,187 - -0.2 2,089,238 + -1.6 2,089,252 + +0.7 2,089,252 + +0.6 2,089,252 + -1.1 2,089,252 + +2.2 2,089,253 - -0.1 2,089,262 + -0.7 2,089,262 + -0.7 2,089,262 + +0.5 2,089,263 - +1.3 2,089,352 + +1.6 2,089,352 + -0.2 2,089,352 + -0.4 2,089,353 - +0.6 2,089,374 + -2.7 2,089,375 - +0.3
Or see this region's nucleotide sequence