Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29050 and RR42_RS29055 are separated by 9 nucleotides RR42_RS29055 and RR42_RS29060 are separated by 133 nucleotides
RR42_RS29050: RR42_RS29050 - glutamate synthase, at 1,872,636 to 1,874,225
_RS29050
RR42_RS29055: RR42_RS29055 - AraC family transcriptional regulator, at 1,874,235 to 1,875,149
_RS29055
RR42_RS29060: RR42_RS29060 - LacI family transcriptional regulator, at 1,875,283 to 1,876,308
_RS29060
Position (kb)
1874
1875
1876 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1873.436 kb on + strand, within RR42_RS29050 at 1874.075 kb on + strand at 1874.076 kb on - strand at 1874.076 kb on - strand at 1874.178 kb on + strand at 1874.179 kb on - strand at 1874.179 kb on - strand at 1874.187 kb on + strand at 1874.187 kb on + strand at 1874.187 kb on + strand at 1874.187 kb on + strand at 1874.224 kb on - strand at 1874.387 kb on + strand, within RR42_RS29055 at 1874.389 kb on + strand, within RR42_RS29055 at 1874.389 kb on + strand, within RR42_RS29055 at 1874.390 kb on - strand, within RR42_RS29055 at 1874.390 kb on - strand, within RR42_RS29055 at 1874.390 kb on - strand, within RR42_RS29055 at 1874.390 kb on - strand, within RR42_RS29055 at 1874.791 kb on + strand, within RR42_RS29055 at 1874.792 kb on - strand, within RR42_RS29055 at 1874.849 kb on - strand, within RR42_RS29055 at 1874.849 kb on - strand, within RR42_RS29055 at 1874.864 kb on + strand, within RR42_RS29055 at 1874.865 kb on - strand, within RR42_RS29055 at 1874.865 kb on - strand, within RR42_RS29055 at 1874.936 kb on + strand, within RR42_RS29055 at 1874.936 kb on + strand, within RR42_RS29055 at 1874.998 kb on + strand, within RR42_RS29055 at 1874.999 kb on - strand, within RR42_RS29055 at 1874.999 kb on - strand, within RR42_RS29055 at 1874.999 kb on - strand, within RR42_RS29055 at 1875.121 kb on + strand at 1875.121 kb on + strand at 1875.131 kb on - strand at 1875.131 kb on - strand at 1875.285 kb on - strand at 1875.285 kb on - strand at 1875.350 kb on + strand at 1875.351 kb on - strand at 1875.707 kb on + strand, within RR42_RS29060 at 1875.707 kb on + strand, within RR42_RS29060 at 1875.707 kb on + strand, within RR42_RS29060 at 1875.708 kb on - strand, within RR42_RS29060 at 1875.824 kb on + strand, within RR42_RS29060 at 1875.933 kb on - strand, within RR42_RS29060 at 1875.947 kb on + strand, within RR42_RS29060 at 1875.948 kb on - strand, within RR42_RS29060 at 1875.948 kb on - strand, within RR42_RS29060 at 1875.948 kb on - strand, within RR42_RS29060 at 1875.995 kb on + strand, within RR42_RS29060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,873,436 + RR42_RS29050 0.50 -2.2 1,874,075 + -3.8 1,874,076 - -2.0 1,874,076 - -0.7 1,874,178 + +0.4 1,874,179 - +1.4 1,874,179 - -0.1 1,874,187 + -0.8 1,874,187 + +0.1 1,874,187 + -0.8 1,874,187 + +2.2 1,874,224 - +0.0 1,874,387 + RR42_RS29055 0.17 +0.1 1,874,389 + RR42_RS29055 0.17 +0.6 1,874,389 + RR42_RS29055 0.17 +0.7 1,874,390 - RR42_RS29055 0.17 +0.6 1,874,390 - RR42_RS29055 0.17 -0.9 1,874,390 - RR42_RS29055 0.17 -0.3 1,874,390 - RR42_RS29055 0.17 +0.2 1,874,791 + RR42_RS29055 0.61 +0.7 1,874,792 - RR42_RS29055 0.61 +0.0 1,874,849 - RR42_RS29055 0.67 +0.3 1,874,849 - RR42_RS29055 0.67 -1.2 1,874,864 + RR42_RS29055 0.69 +0.5 1,874,865 - RR42_RS29055 0.69 -0.8 1,874,865 - RR42_RS29055 0.69 -0.4 1,874,936 + RR42_RS29055 0.77 -1.5 1,874,936 + RR42_RS29055 0.77 -1.0 1,874,998 + RR42_RS29055 0.83 -1.9 1,874,999 - RR42_RS29055 0.83 -0.5 1,874,999 - RR42_RS29055 0.83 +0.5 1,874,999 - RR42_RS29055 0.83 +2.0 1,875,121 + -0.1 1,875,121 + +0.8 1,875,131 - +0.5 1,875,131 - -1.0 1,875,285 - -1.5 1,875,285 - -1.1 1,875,350 + +0.5 1,875,351 - +1.9 1,875,707 + RR42_RS29060 0.41 -1.3 1,875,707 + RR42_RS29060 0.41 -1.4 1,875,707 + RR42_RS29060 0.41 -0.9 1,875,708 - RR42_RS29060 0.41 -1.2 1,875,824 + RR42_RS29060 0.53 +1.8 1,875,933 - RR42_RS29060 0.63 -0.3 1,875,947 + RR42_RS29060 0.65 +0.3 1,875,948 - RR42_RS29060 0.65 +0.3 1,875,948 - RR42_RS29060 0.65 -1.4 1,875,948 - RR42_RS29060 0.65 +0.6 1,875,995 + RR42_RS29060 0.69 -0.4
Or see this region's nucleotide sequence