Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29015 and RR42_RS29020 are separated by 60 nucleotides RR42_RS29020 and RR42_RS29025 are separated by 180 nucleotides RR42_RS29025 and RR42_RS29030 are separated by 188 nucleotides
RR42_RS29015: RR42_RS29015 - IQ calmodulin-binding motif-containing protein, at 1,865,746 to 1,866,765
_RS29015
RR42_RS29020: RR42_RS29020 - hypothetical protein, at 1,866,826 to 1,867,176
_RS29020
RR42_RS29025: RR42_RS29025 - hypothetical protein, at 1,867,357 to 1,867,539
_RS29025
RR42_RS29030: RR42_RS29030 - metal-dependent hydrolase, at 1,867,728 to 1,868,690
_RS29030
Position (kb)
1866
1867
1868 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1865.895 kb on + strand, within RR42_RS29015 at 1865.895 kb on + strand, within RR42_RS29015 at 1865.896 kb on - strand, within RR42_RS29015 at 1865.896 kb on - strand, within RR42_RS29015 at 1865.896 kb on - strand, within RR42_RS29015 at 1865.896 kb on - strand, within RR42_RS29015 at 1866.094 kb on + strand, within RR42_RS29015 at 1866.094 kb on + strand, within RR42_RS29015 at 1866.094 kb on + strand, within RR42_RS29015 at 1866.095 kb on - strand, within RR42_RS29015 at 1866.130 kb on + strand, within RR42_RS29015 at 1866.130 kb on + strand, within RR42_RS29015 at 1866.130 kb on + strand, within RR42_RS29015 at 1866.167 kb on - strand, within RR42_RS29015 at 1866.167 kb on - strand, within RR42_RS29015 at 1866.389 kb on - strand, within RR42_RS29015 at 1866.649 kb on + strand, within RR42_RS29015 at 1866.650 kb on - strand, within RR42_RS29015 at 1866.763 kb on + strand at 1866.886 kb on + strand, within RR42_RS29020 at 1866.905 kb on + strand, within RR42_RS29020 at 1866.905 kb on + strand, within RR42_RS29020 at 1867.381 kb on + strand, within RR42_RS29025 at 1867.601 kb on + strand at 1867.602 kb on - strand at 1867.602 kb on - strand at 1867.602 kb on - strand at 1867.637 kb on - strand at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.980 kb on + strand, within RR42_RS29030 at 1867.980 kb on + strand, within RR42_RS29030 at 1868.046 kb on + strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,865,895 + RR42_RS29015 0.15 -2.7 1,865,895 + RR42_RS29015 0.15 +1.6 1,865,896 - RR42_RS29015 0.15 -2.2 1,865,896 - RR42_RS29015 0.15 +0.4 1,865,896 - RR42_RS29015 0.15 +0.8 1,865,896 - RR42_RS29015 0.15 +0.2 1,866,094 + RR42_RS29015 0.34 +0.6 1,866,094 + RR42_RS29015 0.34 +0.2 1,866,094 + RR42_RS29015 0.34 -0.5 1,866,095 - RR42_RS29015 0.34 -3.0 1,866,130 + RR42_RS29015 0.38 -1.8 1,866,130 + RR42_RS29015 0.38 +0.3 1,866,130 + RR42_RS29015 0.38 -1.2 1,866,167 - RR42_RS29015 0.41 -2.1 1,866,167 - RR42_RS29015 0.41 -0.2 1,866,389 - RR42_RS29015 0.63 -0.6 1,866,649 + RR42_RS29015 0.89 -0.4 1,866,650 - RR42_RS29015 0.89 -0.4 1,866,763 + +0.5 1,866,886 + RR42_RS29020 0.17 +0.1 1,866,905 + RR42_RS29020 0.23 -0.8 1,866,905 + RR42_RS29020 0.23 -0.3 1,867,381 + RR42_RS29025 0.13 -0.1 1,867,601 + +1.5 1,867,602 - -0.7 1,867,602 - -0.9 1,867,602 - -3.2 1,867,637 - +0.3 1,867,827 + RR42_RS29030 0.10 -2.6 1,867,827 + RR42_RS29030 0.10 +1.2 1,867,827 + RR42_RS29030 0.10 +0.9 1,867,827 + RR42_RS29030 0.10 -0.2 1,867,828 - RR42_RS29030 0.10 -0.7 1,867,828 - RR42_RS29030 0.10 -0.6 1,867,828 - RR42_RS29030 0.10 -4.1 1,867,980 + RR42_RS29030 0.26 -0.1 1,867,980 + RR42_RS29030 0.26 -1.0 1,868,046 + RR42_RS29030 0.33 +0.3 1,868,047 - RR42_RS29030 0.33 -1.0 1,868,047 - RR42_RS29030 0.33 -0.6 1,868,047 - RR42_RS29030 0.33 -0.1 1,868,047 - RR42_RS29030 0.33 +1.0
Or see this region's nucleotide sequence