Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS28905

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS28895 and RR42_RS28900 are separated by 57 nucleotidesRR42_RS28900 and RR42_RS28905 are separated by 353 nucleotidesRR42_RS28905 and RR42_RS28910 are separated by 132 nucleotides RR42_RS28895: RR42_RS28895 - acetone carboxylase subunit alpha, at 1,837,664 to 1,839,988 _RS28895 RR42_RS28900: RR42_RS28900 - acetone carboxylase subunit gamma, at 1,840,046 to 1,840,552 _RS28900 RR42_RS28905: RR42_RS28905 - Fis family transcriptional regulator, at 1,840,906 to 1,842,927 _RS28905 RR42_RS28910: RR42_RS28910 - XRE family transcriptional regulator, at 1,843,060 to 1,843,914 _RS28910 Position (kb) 1840 1841 1842 1843Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1839.917 kb on + strandat 1840.019 kb on + strandat 1840.019 kb on + strandat 1840.019 kb on + strandat 1840.020 kb on - strandat 1840.020 kb on - strandat 1840.055 kb on + strandat 1840.055 kb on + strandat 1840.055 kb on + strandat 1840.055 kb on + strandat 1840.055 kb on + strandat 1840.055 kb on + strandat 1840.056 kb on - strandat 1840.123 kb on - strand, within RR42_RS28900at 1840.155 kb on + strand, within RR42_RS28900at 1840.156 kb on - strand, within RR42_RS28900at 1840.158 kb on - strand, within RR42_RS28900at 1840.158 kb on - strand, within RR42_RS28900at 1840.158 kb on - strand, within RR42_RS28900at 1840.213 kb on + strand, within RR42_RS28900at 1840.328 kb on + strand, within RR42_RS28900at 1840.329 kb on - strand, within RR42_RS28900at 1840.404 kb on - strand, within RR42_RS28900at 1840.412 kb on + strand, within RR42_RS28900at 1840.412 kb on + strand, within RR42_RS28900at 1840.413 kb on - strand, within RR42_RS28900at 1840.415 kb on + strand, within RR42_RS28900at 1840.510 kb on + strandat 1840.510 kb on + strandat 1840.796 kb on - strandat 1841.168 kb on + strand, within RR42_RS28905at 1841.169 kb on - strand, within RR42_RS28905at 1841.256 kb on + strand, within RR42_RS28905at 1841.262 kb on + strand, within RR42_RS28905at 1841.262 kb on + strand, within RR42_RS28905at 1841.319 kb on + strand, within RR42_RS28905at 1841.497 kb on + strand, within RR42_RS28905at 1841.498 kb on - strand, within RR42_RS28905at 1841.498 kb on - strand, within RR42_RS28905at 1841.498 kb on - strand, within RR42_RS28905at 1841.498 kb on - strand, within RR42_RS28905at 1841.508 kb on + strand, within RR42_RS28905at 1841.509 kb on - strand, within RR42_RS28905at 1842.272 kb on + strand, within RR42_RS28905at 1842.272 kb on + strand, within RR42_RS28905at 1842.272 kb on + strand, within RR42_RS28905at 1842.272 kb on + strand, within RR42_RS28905at 1842.272 kb on + strand, within RR42_RS28905at 1842.273 kb on - strand, within RR42_RS28905at 1842.314 kb on - strand, within RR42_RS28905at 1842.424 kb on + strand, within RR42_RS28905at 1842.424 kb on + strand, within RR42_RS28905at 1842.424 kb on + strand, within RR42_RS28905at 1842.425 kb on - strand, within RR42_RS28905at 1842.453 kb on - strand, within RR42_RS28905at 1842.515 kb on + strand, within RR42_RS28905at 1842.516 kb on - strand, within RR42_RS28905at 1842.526 kb on + strand, within RR42_RS28905at 1842.527 kb on - strand, within RR42_RS28905at 1842.802 kb on + strandat 1842.802 kb on + strandat 1842.803 kb on - strandat 1842.803 kb on - strandat 1842.803 kb on - strandat 1842.803 kb on - strandat 1842.862 kb on + strandat 1842.881 kb on - strandat 1842.925 kb on + strandat 1842.925 kb on + strandat 1842.925 kb on + strandat 1842.925 kb on + strandat 1842.925 kb on + strandat 1842.926 kb on - strandat 1842.959 kb on + strandat 1843.050 kb on + strandat 1843.050 kb on + strandat 1843.050 kb on + strandat 1843.050 kb on + strandat 1843.051 kb on - strandat 1843.139 kb on - strandat 1843.141 kb on - strandat 1843.186 kb on - strand, within RR42_RS28910at 1843.186 kb on - strand, within RR42_RS28910at 1843.312 kb on + strand, within RR42_RS28910at 1843.312 kb on + strand, within RR42_RS28910at 1843.313 kb on - strand, within RR42_RS28910at 1843.313 kb on - strand, within RR42_RS28910

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,839,917 + -0.2
1,840,019 + +1.2
1,840,019 + -0.7
1,840,019 + -2.7
1,840,020 - -0.5
1,840,020 - +0.2
1,840,055 + +0.9
1,840,055 + -2.4
1,840,055 + +0.6
1,840,055 + -2.0
1,840,055 + +0.5
1,840,055 + -0.7
1,840,056 - +1.1
1,840,123 - RR42_RS28900 0.15 +1.6
1,840,155 + RR42_RS28900 0.21 -1.5
1,840,156 - RR42_RS28900 0.22 -2.5
1,840,158 - RR42_RS28900 0.22 +0.8
1,840,158 - RR42_RS28900 0.22 +0.4
1,840,158 - RR42_RS28900 0.22 -1.4
1,840,213 + RR42_RS28900 0.33 -1.2
1,840,328 + RR42_RS28900 0.56 -1.2
1,840,329 - RR42_RS28900 0.56 +0.3
1,840,404 - RR42_RS28900 0.71 -2.8
1,840,412 + RR42_RS28900 0.72 -1.2
1,840,412 + RR42_RS28900 0.72 -0.6
1,840,413 - RR42_RS28900 0.72 +0.4
1,840,415 + RR42_RS28900 0.73 +0.5
1,840,510 + +1.4
1,840,510 + +1.8
1,840,796 - -2.2
1,841,168 + RR42_RS28905 0.13 -2.8
1,841,169 - RR42_RS28905 0.13 -2.4
1,841,256 + RR42_RS28905 0.17 -1.2
1,841,262 + RR42_RS28905 0.18 +1.6
1,841,262 + RR42_RS28905 0.18 +0.7
1,841,319 + RR42_RS28905 0.20 -1.8
1,841,497 + RR42_RS28905 0.29 +0.3
1,841,498 - RR42_RS28905 0.29 -0.7
1,841,498 - RR42_RS28905 0.29 -2.2
1,841,498 - RR42_RS28905 0.29 +1.3
1,841,498 - RR42_RS28905 0.29 +0.8
1,841,508 + RR42_RS28905 0.30 -0.4
1,841,509 - RR42_RS28905 0.30 +1.8
1,842,272 + RR42_RS28905 0.68 -0.8
1,842,272 + RR42_RS28905 0.68 +1.0
1,842,272 + RR42_RS28905 0.68 +1.7
1,842,272 + RR42_RS28905 0.68 -1.9
1,842,272 + RR42_RS28905 0.68 -0.4
1,842,273 - RR42_RS28905 0.68 -1.2
1,842,314 - RR42_RS28905 0.70 +0.3
1,842,424 + RR42_RS28905 0.75 -0.8
1,842,424 + RR42_RS28905 0.75 +1.7
1,842,424 + RR42_RS28905 0.75 +1.4
1,842,425 - RR42_RS28905 0.75 -1.9
1,842,453 - RR42_RS28905 0.77 -1.8
1,842,515 + RR42_RS28905 0.80 -2.2
1,842,516 - RR42_RS28905 0.80 +1.3
1,842,526 + RR42_RS28905 0.80 +0.1
1,842,527 - RR42_RS28905 0.80 -2.7
1,842,802 + -0.1
1,842,802 + +0.6
1,842,803 - -1.9
1,842,803 - -0.1
1,842,803 - +0.0
1,842,803 - -1.7
1,842,862 + -0.9
1,842,881 - -0.3
1,842,925 + +0.4
1,842,925 + +0.9
1,842,925 + -1.6
1,842,925 + +1.4
1,842,925 + +0.0
1,842,926 - +0.9
1,842,959 + -0.7
1,843,050 + +0.5
1,843,050 + +0.6
1,843,050 + -0.7
1,843,050 + +0.9
1,843,051 - -1.2
1,843,139 - +0.4
1,843,141 - -0.8
1,843,186 - RR42_RS28910 0.15 +0.3
1,843,186 - RR42_RS28910 0.15 -0.3
1,843,312 + RR42_RS28910 0.29 +1.6
1,843,312 + RR42_RS28910 0.29 +1.1
1,843,313 - RR42_RS28910 0.30 +1.3
1,843,313 - RR42_RS28910 0.30 -1.4

Or see this region's nucleotide sequence