Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28285 and RR42_RS28290 are separated by 486 nucleotides RR42_RS28290 and RR42_RS28295 are separated by 88 nucleotides
RR42_RS28285: RR42_RS28285 - cytochrome D ubiquinol oxidase subunit I, at 1,705,624 to 1,707,078
_RS28285
RR42_RS28290: RR42_RS28290 - DNA-binding protein, at 1,707,565 to 1,708,161
_RS28290
RR42_RS28295: RR42_RS28295 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) , at 1,708,250 to 1,709,452
_RS28295
Position (kb)
1707
1708
1709 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1706.629 kb on - strand, within RR42_RS28285 at 1706.629 kb on - strand, within RR42_RS28285 at 1706.849 kb on + strand, within RR42_RS28285 at 1706.918 kb on + strand, within RR42_RS28285 at 1706.918 kb on + strand, within RR42_RS28285 at 1706.918 kb on + strand, within RR42_RS28285 at 1706.919 kb on - strand, within RR42_RS28285 at 1706.931 kb on - strand, within RR42_RS28285 at 1706.978 kb on + strand at 1707.016 kb on - strand at 1707.164 kb on + strand at 1707.164 kb on + strand at 1707.165 kb on - strand at 1707.165 kb on - strand at 1707.168 kb on + strand at 1707.168 kb on + strand at 1707.168 kb on + strand at 1707.168 kb on + strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.169 kb on - strand at 1707.173 kb on + strand at 1707.174 kb on - strand at 1707.174 kb on - strand at 1707.193 kb on + strand at 1707.194 kb on - strand at 1707.194 kb on - strand at 1707.353 kb on + strand at 1707.354 kb on - strand at 1707.360 kb on - strand at 1707.360 kb on - strand at 1707.374 kb on - strand at 1707.479 kb on - strand at 1707.575 kb on + strand at 1707.794 kb on + strand, within RR42_RS28290 at 1707.794 kb on + strand, within RR42_RS28290 at 1707.794 kb on + strand, within RR42_RS28290 at 1707.795 kb on - strand, within RR42_RS28290 at 1708.040 kb on - strand, within RR42_RS28290 at 1708.271 kb on + strand at 1708.272 kb on - strand at 1708.272 kb on - strand at 1708.360 kb on + strand at 1708.360 kb on + strand at 1708.409 kb on + strand, within RR42_RS28295 at 1708.409 kb on + strand, within RR42_RS28295 at 1708.410 kb on - strand, within RR42_RS28295 at 1708.498 kb on + strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.508 kb on - strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1709.014 kb on + strand, within RR42_RS28295 at 1709.140 kb on + strand, within RR42_RS28295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,706,629 - RR42_RS28285 0.69 -1.3 1,706,629 - RR42_RS28285 0.69 -1.8 1,706,849 + RR42_RS28285 0.84 -0.8 1,706,918 + RR42_RS28285 0.89 -0.6 1,706,918 + RR42_RS28285 0.89 +1.3 1,706,918 + RR42_RS28285 0.89 -0.9 1,706,919 - RR42_RS28285 0.89 -2.4 1,706,931 - RR42_RS28285 0.90 -1.5 1,706,978 + -0.4 1,707,016 - +1.3 1,707,164 + -0.1 1,707,164 + -0.4 1,707,165 - +2.6 1,707,165 - +0.9 1,707,168 + -1.0 1,707,168 + +0.1 1,707,168 + +1.7 1,707,168 + -0.3 1,707,169 - +0.4 1,707,169 - +1.2 1,707,169 - -0.3 1,707,169 - +1.1 1,707,169 - +0.7 1,707,169 - +0.1 1,707,169 - -1.2 1,707,169 - +0.7 1,707,173 + -0.5 1,707,174 - +0.7 1,707,174 - -1.5 1,707,193 + -1.7 1,707,194 - -3.2 1,707,194 - +0.3 1,707,353 + -0.5 1,707,354 - -0.2 1,707,360 - +0.7 1,707,360 - +0.4 1,707,374 - +1.6 1,707,479 - -0.7 1,707,575 + +0.6 1,707,794 + RR42_RS28290 0.38 -2.1 1,707,794 + RR42_RS28290 0.38 -2.9 1,707,794 + RR42_RS28290 0.38 -0.8 1,707,795 - RR42_RS28290 0.39 -1.0 1,708,040 - RR42_RS28290 0.80 -4.4 1,708,271 + +1.4 1,708,272 - +0.6 1,708,272 - -0.7 1,708,360 + +1.0 1,708,360 + +1.4 1,708,409 + RR42_RS28295 0.13 -1.3 1,708,409 + RR42_RS28295 0.13 +0.0 1,708,410 - RR42_RS28295 0.13 +0.0 1,708,498 + RR42_RS28295 0.21 +0.5 1,708,499 - RR42_RS28295 0.21 -0.2 1,708,499 - RR42_RS28295 0.21 -1.7 1,708,508 - RR42_RS28295 0.21 -2.8 1,708,693 + RR42_RS28295 0.37 -0.3 1,708,693 + RR42_RS28295 0.37 -0.8 1,709,014 + RR42_RS28295 0.64 +0.1 1,709,140 + RR42_RS28295 0.74 +1.8
Or see this region's nucleotide sequence