Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27660 and RR42_RS27665 are separated by 171 nucleotides RR42_RS27665 and RR42_RS27670 are separated by 33 nucleotides RR42_RS27670 and RR42_RS27675 are separated by 295 nucleotides
RR42_RS27660: RR42_RS27660 - transcriptional regulator, at 1,568,417 to 1,569,085
_RS27660
RR42_RS27665: RR42_RS27665 - endoribonuclease L-PSP, at 1,569,257 to 1,569,721
_RS27665
RR42_RS27670: RR42_RS27670 - LysR family transcriptional regulator, at 1,569,755 to 1,570,711
_RS27670
RR42_RS27675: RR42_RS27675 - esterase, at 1,571,007 to 1,571,930
_RS27675
Position (kb)
1569
1570
1571 Strain fitness (log2 ratio)
-2
-1
0
1 at 1569.096 kb on + strand at 1569.239 kb on + strand at 1569.239 kb on + strand at 1569.239 kb on + strand at 1569.320 kb on + strand, within RR42_RS27665 at 1569.321 kb on - strand, within RR42_RS27665 at 1569.341 kb on + strand, within RR42_RS27665 at 1569.342 kb on - strand, within RR42_RS27665 at 1569.894 kb on + strand, within RR42_RS27670 at 1569.894 kb on + strand, within RR42_RS27670 at 1569.895 kb on - strand, within RR42_RS27670 at 1569.923 kb on - strand, within RR42_RS27670 at 1570.030 kb on - strand, within RR42_RS27670 at 1570.110 kb on - strand, within RR42_RS27670 at 1570.110 kb on - strand, within RR42_RS27670 at 1570.137 kb on + strand, within RR42_RS27670 at 1570.137 kb on + strand, within RR42_RS27670 at 1570.137 kb on + strand, within RR42_RS27670 at 1570.200 kb on + strand, within RR42_RS27670 at 1570.216 kb on - strand, within RR42_RS27670 at 1570.515 kb on + strand, within RR42_RS27670 at 1570.515 kb on + strand, within RR42_RS27670 at 1570.516 kb on - strand, within RR42_RS27670 at 1570.518 kb on + strand, within RR42_RS27670 at 1570.519 kb on - strand, within RR42_RS27670 at 1570.886 kb on - strand at 1570.926 kb on - strand at 1570.948 kb on + strand at 1570.948 kb on + strand at 1570.948 kb on + strand at 1570.948 kb on + strand at 1570.949 kb on - strand at 1570.967 kb on - strand at 1571.044 kb on + strand at 1571.045 kb on - strand at 1571.125 kb on - strand, within RR42_RS27675 at 1571.178 kb on + strand, within RR42_RS27675 at 1571.178 kb on + strand, within RR42_RS27675 at 1571.178 kb on + strand, within RR42_RS27675 at 1571.586 kb on + strand, within RR42_RS27675 at 1571.587 kb on - strand, within RR42_RS27675 at 1571.590 kb on - strand, within RR42_RS27675 at 1571.598 kb on + strand, within RR42_RS27675 at 1571.632 kb on - strand, within RR42_RS27675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,569,096 + +0.6 1,569,239 + +0.2 1,569,239 + -2.2 1,569,239 + +0.6 1,569,320 + RR42_RS27665 0.14 +0.6 1,569,321 - RR42_RS27665 0.14 +0.0 1,569,341 + RR42_RS27665 0.18 +0.9 1,569,342 - RR42_RS27665 0.18 -0.1 1,569,894 + RR42_RS27670 0.15 -2.6 1,569,894 + RR42_RS27670 0.15 -0.1 1,569,895 - RR42_RS27670 0.15 -0.3 1,569,923 - RR42_RS27670 0.18 -0.8 1,570,030 - RR42_RS27670 0.29 +0.9 1,570,110 - RR42_RS27670 0.37 +1.4 1,570,110 - RR42_RS27670 0.37 -0.2 1,570,137 + RR42_RS27670 0.40 -0.2 1,570,137 + RR42_RS27670 0.40 -0.0 1,570,137 + RR42_RS27670 0.40 +0.8 1,570,200 + RR42_RS27670 0.46 -0.0 1,570,216 - RR42_RS27670 0.48 -0.6 1,570,515 + RR42_RS27670 0.79 -2.2 1,570,515 + RR42_RS27670 0.79 +0.5 1,570,516 - RR42_RS27670 0.80 +0.1 1,570,518 + RR42_RS27670 0.80 -1.6 1,570,519 - RR42_RS27670 0.80 -0.8 1,570,886 - -0.2 1,570,926 - +0.2 1,570,948 + -0.3 1,570,948 + -0.4 1,570,948 + -1.0 1,570,948 + -0.0 1,570,949 - +0.1 1,570,967 - -0.5 1,571,044 + +0.9 1,571,045 - +0.7 1,571,125 - RR42_RS27675 0.13 -2.1 1,571,178 + RR42_RS27675 0.19 +1.2 1,571,178 + RR42_RS27675 0.19 +1.7 1,571,178 + RR42_RS27675 0.19 -1.5 1,571,586 + RR42_RS27675 0.63 -0.0 1,571,587 - RR42_RS27675 0.63 +1.2 1,571,590 - RR42_RS27675 0.63 +0.9 1,571,598 + RR42_RS27675 0.64 -0.0 1,571,632 - RR42_RS27675 0.68 -1.2
Or see this region's nucleotide sequence