Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27640 and RR42_RS27645 are separated by 89 nucleotides RR42_RS27645 and RR42_RS27650 are separated by 75 nucleotides
RR42_RS27640: RR42_RS27640 - CDP-diacylglycerol pyrophosphatase, at 1,565,000 to 1,565,776
_RS27640
RR42_RS27645: RR42_RS27645 - hypothetical protein, at 1,565,866 to 1,566,120
_RS27645
RR42_RS27650: RR42_RS27650 - phosphate-binding protein, at 1,566,196 to 1,567,179
_RS27650
Position (kb) 1565
1566
1567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1565.013 kb on + strand at 1565.013 kb on + strand at 1565.014 kb on - strand at 1565.175 kb on - strand, within RR42_RS27640 at 1565.395 kb on + strand, within RR42_RS27640 at 1565.455 kb on + strand, within RR42_RS27640 at 1565.499 kb on + strand, within RR42_RS27640 at 1565.499 kb on + strand, within RR42_RS27640 at 1565.618 kb on + strand, within RR42_RS27640 at 1565.618 kb on + strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.775 kb on - strand at 1565.827 kb on + strand at 1565.876 kb on - strand at 1565.876 kb on - strand at 1566.053 kb on - strand, within RR42_RS27645 at 1566.067 kb on + strand, within RR42_RS27645 at 1566.068 kb on - strand, within RR42_RS27645 at 1566.260 kb on + strand at 1566.261 kb on - strand at 1566.425 kb on + strand, within RR42_RS27650 at 1566.426 kb on - strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.566 kb on + strand, within RR42_RS27650 at 1566.567 kb on - strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.597 kb on - strand, within RR42_RS27650 at 1566.701 kb on + strand, within RR42_RS27650 at 1566.833 kb on + strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.838 kb on - strand, within RR42_RS27650 at 1567.046 kb on + strand, within RR42_RS27650 at 1567.047 kb on - strand, within RR42_RS27650 at 1567.074 kb on - strand, within RR42_RS27650 at 1567.074 kb on - strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.077 kb on - strand, within RR42_RS27650 at 1567.077 kb on - strand, within RR42_RS27650 at 1567.106 kb on + strand at 1567.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,565,013 + +1.5 1,565,013 + -1.3 1,565,014 - +0.6 1,565,175 - RR42_RS27640 0.23 +0.1 1,565,395 + RR42_RS27640 0.51 -0.4 1,565,455 + RR42_RS27640 0.59 -1.1 1,565,499 + RR42_RS27640 0.64 -1.1 1,565,499 + RR42_RS27640 0.64 +1.0 1,565,618 + RR42_RS27640 0.80 +1.2 1,565,618 + RR42_RS27640 0.80 -2.1 1,565,619 - RR42_RS27640 0.80 +1.5 1,565,619 - RR42_RS27640 0.80 -0.2 1,565,619 - RR42_RS27640 0.80 -2.3 1,565,619 - RR42_RS27640 0.80 +1.0 1,565,619 - RR42_RS27640 0.80 +0.6 1,565,775 - -1.6 1,565,827 + -0.5 1,565,876 - +2.4 1,565,876 - +0.6 1,566,053 - RR42_RS27645 0.73 -0.2 1,566,067 + RR42_RS27645 0.79 +1.1 1,566,068 - RR42_RS27645 0.79 -0.3 1,566,260 + +1.7 1,566,261 - +0.8 1,566,425 + RR42_RS27650 0.23 +0.6 1,566,426 - RR42_RS27650 0.23 +1.1 1,566,551 + RR42_RS27650 0.36 -0.9 1,566,551 + RR42_RS27650 0.36 +1.7 1,566,566 + RR42_RS27650 0.38 +0.3 1,566,567 - RR42_RS27650 0.38 -1.1 1,566,596 + RR42_RS27650 0.41 -3.0 1,566,596 + RR42_RS27650 0.41 -2.5 1,566,597 - RR42_RS27650 0.41 -2.6 1,566,701 + RR42_RS27650 0.51 -1.2 1,566,833 + RR42_RS27650 0.65 +1.7 1,566,834 - RR42_RS27650 0.65 -0.1 1,566,834 - RR42_RS27650 0.65 -0.9 1,566,834 - RR42_RS27650 0.65 +0.8 1,566,838 - RR42_RS27650 0.65 -0.5 1,567,046 + RR42_RS27650 0.86 -2.3 1,567,047 - RR42_RS27650 0.86 -0.8 1,567,074 - RR42_RS27650 0.89 -2.1 1,567,074 - RR42_RS27650 0.89 +0.1 1,567,076 + RR42_RS27650 0.89 -0.2 1,567,076 + RR42_RS27650 0.89 -1.0 1,567,076 + RR42_RS27650 0.89 -0.8 1,567,077 - RR42_RS27650 0.90 -1.3 1,567,077 - RR42_RS27650 0.90 -0.1 1,567,106 + +0.2 1,567,106 + +1.0
Or see this region's nucleotide sequence