Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27360 and RR42_RS27365 are separated by 33 nucleotides RR42_RS27365 and RR42_RS27370 are separated by 66 nucleotides RR42_RS27370 and RR42_RS27375 are separated by 223 nucleotides
RR42_RS27360: RR42_RS27360 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 1,491,240 to 1,492,238
_RS27360
RR42_RS27365: RR42_RS27365 - DSBA oxidoreductase, at 1,492,272 to 1,492,793
_RS27365
RR42_RS27370: RR42_RS27370 - zinc finger UBP-type protein, at 1,492,860 to 1,493,120
_RS27370
RR42_RS27375: RR42_RS27375 - NAD(P)H-quinone oxidoreductase, at 1,493,344 to 1,493,964
_RS27375
Position (kb)
1492
1493 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1491.627 kb on + strand, within RR42_RS27360 at 1491.642 kb on + strand, within RR42_RS27360 at 1491.643 kb on - strand, within RR42_RS27360 at 1491.747 kb on + strand, within RR42_RS27360 at 1491.822 kb on + strand, within RR42_RS27360 at 1492.024 kb on - strand, within RR42_RS27360 at 1492.128 kb on + strand, within RR42_RS27360 at 1492.128 kb on + strand, within RR42_RS27360 at 1492.128 kb on + strand, within RR42_RS27360 at 1492.129 kb on - strand, within RR42_RS27360 at 1492.203 kb on - strand at 1492.207 kb on - strand at 1492.207 kb on - strand at 1492.207 kb on - strand at 1492.441 kb on + strand, within RR42_RS27365 at 1492.441 kb on + strand, within RR42_RS27365 at 1492.442 kb on - strand, within RR42_RS27365 at 1492.447 kb on + strand, within RR42_RS27365 at 1492.473 kb on - strand, within RR42_RS27365 at 1492.473 kb on - strand, within RR42_RS27365 at 1492.481 kb on - strand, within RR42_RS27365 at 1492.654 kb on + strand, within RR42_RS27365 at 1492.700 kb on - strand at 1492.721 kb on - strand, within RR42_RS27365 at 1492.758 kb on + strand at 1492.758 kb on + strand at 1492.758 kb on + strand at 1492.759 kb on - strand at 1492.759 kb on - strand at 1492.883 kb on + strand at 1492.885 kb on + strand at 1492.886 kb on - strand, within RR42_RS27370 at 1492.886 kb on - strand, within RR42_RS27370 at 1492.886 kb on - strand, within RR42_RS27370 at 1493.188 kb on - strand at 1493.188 kb on - strand at 1493.188 kb on - strand at 1493.251 kb on + strand at 1493.371 kb on + strand at 1493.371 kb on + strand at 1493.371 kb on + strand at 1493.372 kb on - strand at 1493.372 kb on - strand at 1493.372 kb on - strand at 1493.381 kb on - strand at 1493.381 kb on - strand at 1493.393 kb on - strand at 1493.607 kb on + strand, within RR42_RS27375 at 1493.607 kb on + strand, within RR42_RS27375 at 1493.607 kb on + strand, within RR42_RS27375 at 1493.608 kb on - strand, within RR42_RS27375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,491,627 + RR42_RS27360 0.39 +0.1 1,491,642 + RR42_RS27360 0.40 +0.0 1,491,643 - RR42_RS27360 0.40 -0.7 1,491,747 + RR42_RS27360 0.51 -2.7 1,491,822 + RR42_RS27360 0.58 -0.6 1,492,024 - RR42_RS27360 0.78 +0.2 1,492,128 + RR42_RS27360 0.89 +0.4 1,492,128 + RR42_RS27360 0.89 +0.2 1,492,128 + RR42_RS27360 0.89 +0.7 1,492,129 - RR42_RS27360 0.89 -1.0 1,492,203 - +0.7 1,492,207 - -0.3 1,492,207 - -1.9 1,492,207 - -0.6 1,492,441 + RR42_RS27365 0.32 +1.3 1,492,441 + RR42_RS27365 0.32 +0.9 1,492,442 - RR42_RS27365 0.33 +0.8 1,492,447 + RR42_RS27365 0.34 +1.9 1,492,473 - RR42_RS27365 0.39 -0.5 1,492,473 - RR42_RS27365 0.39 +0.1 1,492,481 - RR42_RS27365 0.40 -0.0 1,492,654 + RR42_RS27365 0.73 -4.4 1,492,700 - -1.6 1,492,721 - RR42_RS27365 0.86 -3.7 1,492,758 + -0.1 1,492,758 + +0.3 1,492,758 + +0.8 1,492,759 - -0.3 1,492,759 - +0.7 1,492,883 + +0.3 1,492,885 + -1.4 1,492,886 - RR42_RS27370 0.10 +0.6 1,492,886 - RR42_RS27370 0.10 +0.1 1,492,886 - RR42_RS27370 0.10 +0.8 1,493,188 - -1.2 1,493,188 - +0.1 1,493,188 - +0.7 1,493,251 + -0.2 1,493,371 + -1.0 1,493,371 + +1.2 1,493,371 + -0.9 1,493,372 - +0.2 1,493,372 - +0.6 1,493,372 - +1.6 1,493,381 - -0.6 1,493,381 - +0.3 1,493,393 - -0.2 1,493,607 + RR42_RS27375 0.42 -0.3 1,493,607 + RR42_RS27375 0.42 +0.6 1,493,607 + RR42_RS27375 0.42 -1.7 1,493,608 - RR42_RS27375 0.43 +1.1
Or see this region's nucleotide sequence