Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27345 and RR42_RS27350 are separated by 76 nucleotides RR42_RS27350 and RR42_RS27355 are separated by 78 nucleotides RR42_RS27355 and RR42_RS27360 are separated by 360 nucleotides
RR42_RS27345: RR42_RS27345 - aldehyde dehydrogenase, at 1,488,053 to 1,488,784
_RS27345
RR42_RS27350: RR42_RS27350 - aldehyde dehydrogenase, at 1,488,861 to 1,490,321
_RS27350
RR42_RS27355: RR42_RS27355 - CDP-alcohol phosphatidyltransferase, at 1,490,400 to 1,490,879
_RS27355
RR42_RS27360: RR42_RS27360 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 1,491,240 to 1,492,238
_RS27360
Position (kb)
1488
1489
1490
1491 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1488.008 kb on - strand at 1488.894 kb on + strand at 1488.894 kb on + strand at 1488.894 kb on + strand at 1488.894 kb on + strand at 1488.894 kb on + strand at 1488.894 kb on + strand at 1488.894 kb on + strand at 1488.895 kb on - strand at 1488.895 kb on - strand at 1488.895 kb on - strand at 1488.895 kb on - strand at 1489.101 kb on + strand, within RR42_RS27350 at 1489.212 kb on + strand, within RR42_RS27350 at 1489.213 kb on - strand, within RR42_RS27350 at 1489.249 kb on - strand, within RR42_RS27350 at 1489.249 kb on - strand, within RR42_RS27350 at 1489.278 kb on + strand, within RR42_RS27350 at 1489.577 kb on + strand, within RR42_RS27350 at 1489.577 kb on + strand, within RR42_RS27350 at 1489.767 kb on + strand, within RR42_RS27350 at 1489.769 kb on + strand, within RR42_RS27350 at 1489.770 kb on - strand, within RR42_RS27350 at 1489.881 kb on + strand, within RR42_RS27350 at 1489.962 kb on + strand, within RR42_RS27350 at 1489.963 kb on - strand, within RR42_RS27350 at 1490.089 kb on - strand, within RR42_RS27350 at 1490.097 kb on + strand, within RR42_RS27350 at 1490.098 kb on - strand, within RR42_RS27350 at 1490.136 kb on + strand, within RR42_RS27350 at 1490.137 kb on - strand, within RR42_RS27350 at 1490.307 kb on + strand at 1490.877 kb on + strand at 1490.877 kb on + strand at 1490.956 kb on - strand at 1490.956 kb on - strand at 1491.035 kb on + strand at 1491.193 kb on - strand at 1491.193 kb on - strand at 1491.199 kb on + strand at 1491.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,488,008 - -0.9 1,488,894 + +0.3 1,488,894 + -0.7 1,488,894 + +0.0 1,488,894 + +0.9 1,488,894 + -0.0 1,488,894 + +0.7 1,488,894 + -2.8 1,488,895 - -0.9 1,488,895 - +1.1 1,488,895 - +0.1 1,488,895 - -1.3 1,489,101 + RR42_RS27350 0.16 -0.0 1,489,212 + RR42_RS27350 0.24 -1.3 1,489,213 - RR42_RS27350 0.24 +0.6 1,489,249 - RR42_RS27350 0.27 +0.7 1,489,249 - RR42_RS27350 0.27 -1.3 1,489,278 + RR42_RS27350 0.29 -1.9 1,489,577 + RR42_RS27350 0.49 -1.3 1,489,577 + RR42_RS27350 0.49 -1.8 1,489,767 + RR42_RS27350 0.62 -0.8 1,489,769 + RR42_RS27350 0.62 -0.2 1,489,770 - RR42_RS27350 0.62 -1.4 1,489,881 + RR42_RS27350 0.70 +0.0 1,489,962 + RR42_RS27350 0.75 +0.5 1,489,963 - RR42_RS27350 0.75 +0.9 1,490,089 - RR42_RS27350 0.84 +0.2 1,490,097 + RR42_RS27350 0.85 -1.2 1,490,098 - RR42_RS27350 0.85 +1.0 1,490,136 + RR42_RS27350 0.87 -0.3 1,490,137 - RR42_RS27350 0.87 -3.6 1,490,307 + -0.7 1,490,877 + -0.7 1,490,877 + +0.2 1,490,956 - -0.3 1,490,956 - -0.2 1,491,035 + -1.4 1,491,193 - +0.6 1,491,193 - -0.8 1,491,199 + +0.8 1,491,206 - -0.4
Or see this region's nucleotide sequence