Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27255 and RR42_RS27260 are separated by 424 nucleotides RR42_RS27260 and RR42_RS27265 are separated by 99 nucleotides
RR42_RS27255: RR42_RS27255 - hypothetical protein, at 1,467,592 to 1,468,338
_RS27255
RR42_RS27260: RR42_RS27260 - hypothetical protein, at 1,468,763 to 1,469,890
_RS27260
RR42_RS27265: RR42_RS27265 - LacI family transcriptional regulator, at 1,469,990 to 1,470,955
_RS27265
Position (kb)
1468
1469
1470 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1467.779 kb on - strand, within RR42_RS27255 at 1467.779 kb on - strand, within RR42_RS27255 at 1467.779 kb on - strand, within RR42_RS27255 at 1467.908 kb on + strand, within RR42_RS27255 at 1467.967 kb on - strand, within RR42_RS27255 at 1468.060 kb on + strand, within RR42_RS27255 at 1468.060 kb on + strand, within RR42_RS27255 at 1468.060 kb on + strand, within RR42_RS27255 at 1468.061 kb on - strand, within RR42_RS27255 at 1468.063 kb on + strand, within RR42_RS27255 at 1468.064 kb on - strand, within RR42_RS27255 at 1468.071 kb on + strand, within RR42_RS27255 at 1468.096 kb on + strand, within RR42_RS27255 at 1468.097 kb on - strand, within RR42_RS27255 at 1468.097 kb on - strand, within RR42_RS27255 at 1468.100 kb on + strand, within RR42_RS27255 at 1468.100 kb on + strand, within RR42_RS27255 at 1468.100 kb on + strand, within RR42_RS27255 at 1468.100 kb on + strand, within RR42_RS27255 at 1468.100 kb on + strand, within RR42_RS27255 at 1468.100 kb on + strand, within RR42_RS27255 at 1468.131 kb on + strand, within RR42_RS27255 at 1468.131 kb on + strand, within RR42_RS27255 at 1468.207 kb on + strand, within RR42_RS27255 at 1468.208 kb on - strand, within RR42_RS27255 at 1468.274 kb on + strand at 1468.278 kb on + strand at 1468.278 kb on + strand at 1468.279 kb on - strand at 1468.279 kb on - strand at 1468.337 kb on - strand at 1468.337 kb on - strand at 1468.491 kb on - strand at 1468.506 kb on - strand at 1468.513 kb on - strand at 1468.669 kb on - strand at 1468.669 kb on - strand at 1468.675 kb on + strand at 1468.868 kb on + strand at 1468.868 kb on + strand at 1468.869 kb on - strand at 1468.869 kb on - strand at 1468.947 kb on - strand, within RR42_RS27260 at 1468.947 kb on - strand, within RR42_RS27260 at 1469.006 kb on - strand, within RR42_RS27260 at 1469.018 kb on + strand, within RR42_RS27260 at 1469.019 kb on - strand, within RR42_RS27260 at 1469.019 kb on - strand, within RR42_RS27260 at 1469.204 kb on + strand, within RR42_RS27260 at 1469.327 kb on - strand, within RR42_RS27260 at 1469.499 kb on - strand, within RR42_RS27260 at 1469.499 kb on - strand, within RR42_RS27260 at 1469.523 kb on - strand, within RR42_RS27260 at 1469.523 kb on - strand, within RR42_RS27260 at 1469.523 kb on - strand, within RR42_RS27260 at 1469.630 kb on + strand, within RR42_RS27260 at 1469.630 kb on + strand, within RR42_RS27260 at 1469.631 kb on - strand, within RR42_RS27260 at 1469.631 kb on - strand, within RR42_RS27260 at 1469.704 kb on + strand, within RR42_RS27260 at 1469.704 kb on + strand, within RR42_RS27260 at 1469.705 kb on - strand, within RR42_RS27260 at 1469.705 kb on - strand, within RR42_RS27260 at 1469.763 kb on - strand, within RR42_RS27260 at 1469.763 kb on - strand, within RR42_RS27260 at 1469.763 kb on - strand, within RR42_RS27260 at 1469.949 kb on - strand at 1470.027 kb on + strand at 1470.027 kb on + strand at 1470.027 kb on + strand at 1470.028 kb on - strand at 1470.050 kb on - strand at 1470.128 kb on + strand, within RR42_RS27265 at 1470.129 kb on - strand, within RR42_RS27265 at 1470.154 kb on - strand, within RR42_RS27265 at 1470.204 kb on + strand, within RR42_RS27265 at 1470.254 kb on + strand, within RR42_RS27265 at 1470.254 kb on + strand, within RR42_RS27265 at 1470.254 kb on + strand, within RR42_RS27265 at 1470.408 kb on + strand, within RR42_RS27265 at 1470.408 kb on + strand, within RR42_RS27265 at 1470.409 kb on - strand, within RR42_RS27265 at 1470.409 kb on - strand, within RR42_RS27265 at 1470.495 kb on + strand, within RR42_RS27265 at 1470.496 kb on - strand, within RR42_RS27265 at 1470.552 kb on + strand, within RR42_RS27265 at 1470.552 kb on + strand, within RR42_RS27265 at 1470.553 kb on - strand, within RR42_RS27265 at 1470.559 kb on + strand, within RR42_RS27265 at 1470.634 kb on - strand, within RR42_RS27265 at 1470.648 kb on + strand, within RR42_RS27265 at 1470.649 kb on - strand, within RR42_RS27265 at 1470.649 kb on - strand, within RR42_RS27265 at 1470.700 kb on - strand, within RR42_RS27265 at 1470.883 kb on - strand at 1470.883 kb on - strand at 1470.883 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,467,779 - RR42_RS27255 0.25 -1.4 1,467,779 - RR42_RS27255 0.25 -3.1 1,467,779 - RR42_RS27255 0.25 +1.5 1,467,908 + RR42_RS27255 0.42 -0.9 1,467,967 - RR42_RS27255 0.50 -1.1 1,468,060 + RR42_RS27255 0.63 -2.9 1,468,060 + RR42_RS27255 0.63 +0.4 1,468,060 + RR42_RS27255 0.63 +0.7 1,468,061 - RR42_RS27255 0.63 +0.5 1,468,063 + RR42_RS27255 0.63 -0.4 1,468,064 - RR42_RS27255 0.63 -0.1 1,468,071 + RR42_RS27255 0.64 +0.2 1,468,096 + RR42_RS27255 0.67 -1.5 1,468,097 - RR42_RS27255 0.68 +1.3 1,468,097 - RR42_RS27255 0.68 +0.0 1,468,100 + RR42_RS27255 0.68 +2.1 1,468,100 + RR42_RS27255 0.68 -0.1 1,468,100 + RR42_RS27255 0.68 -2.1 1,468,100 + RR42_RS27255 0.68 -0.1 1,468,100 + RR42_RS27255 0.68 +1.0 1,468,100 + RR42_RS27255 0.68 +0.4 1,468,131 + RR42_RS27255 0.72 +0.8 1,468,131 + RR42_RS27255 0.72 -0.1 1,468,207 + RR42_RS27255 0.82 +1.0 1,468,208 - RR42_RS27255 0.82 -0.1 1,468,274 + -0.0 1,468,278 + -0.9 1,468,278 + -0.0 1,468,279 - -0.0 1,468,279 - -0.9 1,468,337 - +1.5 1,468,337 - +0.6 1,468,491 - +0.1 1,468,506 - -0.1 1,468,513 - +0.1 1,468,669 - +0.7 1,468,669 - +0.2 1,468,675 + +0.4 1,468,868 + -1.1 1,468,868 + -0.2 1,468,869 - -3.3 1,468,869 - +0.5 1,468,947 - RR42_RS27260 0.16 +1.5 1,468,947 - RR42_RS27260 0.16 -0.5 1,469,006 - RR42_RS27260 0.22 +0.9 1,469,018 + RR42_RS27260 0.23 -2.7 1,469,019 - RR42_RS27260 0.23 -1.6 1,469,019 - RR42_RS27260 0.23 -0.5 1,469,204 + RR42_RS27260 0.39 -0.8 1,469,327 - RR42_RS27260 0.50 +0.2 1,469,499 - RR42_RS27260 0.65 -0.1 1,469,499 - RR42_RS27260 0.65 +0.5 1,469,523 - RR42_RS27260 0.67 -0.3 1,469,523 - RR42_RS27260 0.67 +2.5 1,469,523 - RR42_RS27260 0.67 +0.8 1,469,630 + RR42_RS27260 0.77 -1.3 1,469,630 + RR42_RS27260 0.77 +0.3 1,469,631 - RR42_RS27260 0.77 +0.5 1,469,631 - RR42_RS27260 0.77 -0.5 1,469,704 + RR42_RS27260 0.83 +1.1 1,469,704 + RR42_RS27260 0.83 +1.2 1,469,705 - RR42_RS27260 0.84 +0.3 1,469,705 - RR42_RS27260 0.84 -0.3 1,469,763 - RR42_RS27260 0.89 -0.8 1,469,763 - RR42_RS27260 0.89 +1.1 1,469,763 - RR42_RS27260 0.89 +3.7 1,469,949 - +1.7 1,470,027 + -0.3 1,470,027 + +0.3 1,470,027 + -0.4 1,470,028 - +0.5 1,470,050 - -0.7 1,470,128 + RR42_RS27265 0.14 +0.5 1,470,129 - RR42_RS27265 0.14 -1.0 1,470,154 - RR42_RS27265 0.17 -0.1 1,470,204 + RR42_RS27265 0.22 -0.7 1,470,254 + RR42_RS27265 0.27 -0.9 1,470,254 + RR42_RS27265 0.27 +1.5 1,470,254 + RR42_RS27265 0.27 +1.1 1,470,408 + RR42_RS27265 0.43 -0.5 1,470,408 + RR42_RS27265 0.43 -0.4 1,470,409 - RR42_RS27265 0.43 +0.4 1,470,409 - RR42_RS27265 0.43 +0.5 1,470,495 + RR42_RS27265 0.52 -0.3 1,470,496 - RR42_RS27265 0.52 +0.7 1,470,552 + RR42_RS27265 0.58 +0.7 1,470,552 + RR42_RS27265 0.58 +0.7 1,470,553 - RR42_RS27265 0.58 +0.8 1,470,559 + RR42_RS27265 0.59 -1.3 1,470,634 - RR42_RS27265 0.67 +0.1 1,470,648 + RR42_RS27265 0.68 +0.2 1,470,649 - RR42_RS27265 0.68 -2.5 1,470,649 - RR42_RS27265 0.68 -1.3 1,470,700 - RR42_RS27265 0.73 -0.4 1,470,883 - +0.9 1,470,883 - -1.0 1,470,883 - -0.0
Or see this region's nucleotide sequence