Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26895 and RR42_RS26900 are separated by 82 nucleotides RR42_RS26900 and RR42_RS26905 are separated by 50 nucleotides RR42_RS26905 and RR42_RS26910 are separated by 68 nucleotides
RR42_RS26895: RR42_RS26895 - 3-methylcrotonyl-CoA carboxylase, at 1,387,783 to 1,389,807
_RS26895
RR42_RS26900: RR42_RS26900 - enoyl-CoA hydratase, at 1,389,890 to 1,390,678
_RS26900
RR42_RS26905: RR42_RS26905 - methylcrotonoyl-CoA carboxylase, at 1,390,729 to 1,392,336
_RS26905
RR42_RS26910: RR42_RS26910 - isovaleryl-CoA dehydrogenase, at 1,392,405 to 1,393,586
_RS26910
Position (kb)
1390
1391
1392
1393 Strain fitness (log2 ratio)
-2
-1
0
1 at 1390.233 kb on + strand, within RR42_RS26900 at 1390.347 kb on + strand, within RR42_RS26900 at 1390.347 kb on + strand, within RR42_RS26900 at 1390.661 kb on - strand at 1391.468 kb on + strand, within RR42_RS26905 at 1391.756 kb on + strand, within RR42_RS26905 at 1391.835 kb on - strand, within RR42_RS26905 at 1391.915 kb on + strand, within RR42_RS26905 at 1391.987 kb on + strand, within RR42_RS26905 at 1391.988 kb on - strand, within RR42_RS26905 at 1391.988 kb on - strand, within RR42_RS26905 at 1391.988 kb on - strand, within RR42_RS26905 at 1392.092 kb on + strand, within RR42_RS26905 at 1392.148 kb on + strand, within RR42_RS26905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,390,233 + RR42_RS26900 0.43 -0.3 1,390,347 + RR42_RS26900 0.58 -1.9 1,390,347 + RR42_RS26900 0.58 -2.7 1,390,661 - -0.8 1,391,468 + RR42_RS26905 0.46 +1.2 1,391,756 + RR42_RS26905 0.64 -0.9 1,391,835 - RR42_RS26905 0.69 +0.7 1,391,915 + RR42_RS26905 0.74 -1.1 1,391,987 + RR42_RS26905 0.78 -1.0 1,391,988 - RR42_RS26905 0.78 -2.3 1,391,988 - RR42_RS26905 0.78 -1.3 1,391,988 - RR42_RS26905 0.78 -0.8 1,392,092 + RR42_RS26905 0.85 -1.5 1,392,148 + RR42_RS26905 0.88 -1.8
Or see this region's nucleotide sequence