Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26425 and RR42_RS26430 are separated by 14 nucleotides RR42_RS26430 and RR42_RS26435 are separated by 9 nucleotides RR42_RS26435 and RR42_RS26440 are separated by 74 nucleotides
RR42_RS26425: RR42_RS26425 - biphenyl 2,3-dioxygenase, at 1,285,066 to 1,286,355
_RS26425
RR42_RS26430: RR42_RS26430 - ferredoxin reductase, at 1,286,370 to 1,287,656
_RS26430
RR42_RS26435: RR42_RS26435 - ferredoxin, at 1,287,666 to 1,287,983
_RS26435
RR42_RS26440: RR42_RS26440 - ABC transporter permease, at 1,288,058 to 1,289,116
_RS26440
Position (kb) 1286
1287
1288 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1285.739 kb on + strand, within RR42_RS26425 at 1285.806 kb on + strand, within RR42_RS26425 at 1285.806 kb on + strand, within RR42_RS26425 at 1285.807 kb on - strand, within RR42_RS26425 at 1285.808 kb on + strand, within RR42_RS26425 at 1285.809 kb on - strand, within RR42_RS26425 at 1285.809 kb on - strand, within RR42_RS26425 at 1285.838 kb on + strand, within RR42_RS26425 at 1286.247 kb on - strand at 1286.351 kb on + strand at 1286.352 kb on - strand at 1286.352 kb on - strand at 1286.618 kb on - strand, within RR42_RS26430 at 1286.654 kb on + strand, within RR42_RS26430 at 1287.353 kb on - strand, within RR42_RS26430 at 1287.529 kb on + strand at 1287.824 kb on - strand, within RR42_RS26435 at 1287.844 kb on + strand, within RR42_RS26435 at 1287.896 kb on - strand, within RR42_RS26435 at 1288.250 kb on - strand, within RR42_RS26440 at 1288.641 kb on + strand, within RR42_RS26440 at 1288.644 kb on + strand, within RR42_RS26440 at 1288.645 kb on - strand, within RR42_RS26440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,285,739 + RR42_RS26425 0.52 -0.4 1,285,806 + RR42_RS26425 0.57 +1.1 1,285,806 + RR42_RS26425 0.57 +0.6 1,285,807 - RR42_RS26425 0.57 -0.0 1,285,808 + RR42_RS26425 0.58 +1.1 1,285,809 - RR42_RS26425 0.58 +0.2 1,285,809 - RR42_RS26425 0.58 -3.4 1,285,838 + RR42_RS26425 0.60 -0.4 1,286,247 - +0.9 1,286,351 + -0.4 1,286,352 - +0.4 1,286,352 - -1.7 1,286,618 - RR42_RS26430 0.19 -2.7 1,286,654 + RR42_RS26430 0.22 -0.1 1,287,353 - RR42_RS26430 0.76 -3.1 1,287,529 + +0.4 1,287,824 - RR42_RS26435 0.50 +0.3 1,287,844 + RR42_RS26435 0.56 +1.4 1,287,896 - RR42_RS26435 0.72 -0.5 1,288,250 - RR42_RS26440 0.18 +0.0 1,288,641 + RR42_RS26440 0.55 +0.1 1,288,644 + RR42_RS26440 0.55 +0.3 1,288,645 - RR42_RS26440 0.55 +0.5
Or see this region's nucleotide sequence