Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26215 and RR42_RS26220 are separated by 182 nucleotides RR42_RS26220 and RR42_RS26225 overlap by 4 nucleotides RR42_RS26225 and RR42_RS26230 are separated by 69 nucleotides
RR42_RS26215: RR42_RS26215 - diguanylate cyclase, at 1,238,848 to 1,239,825
_RS26215
RR42_RS26220: RR42_RS26220 - citrate lyase subunit beta, at 1,240,008 to 1,240,925
_RS26220
RR42_RS26225: RR42_RS26225 - dimethylmenaquinone methyltransferase, at 1,240,922 to 1,241,578
_RS26225
RR42_RS26230: RR42_RS26230 - MFS transporter, at 1,241,648 to 1,242,919
_RS26230
Position (kb)
1240
1241 Strain fitness (log2 ratio)
-2
-1
0
1 at 1239.781 kb on + strand at 1239.781 kb on + strand at 1239.781 kb on + strand at 1239.781 kb on + strand at 1239.781 kb on + strand at 1239.781 kb on + strand at 1239.781 kb on + strand at 1239.782 kb on - strand at 1239.783 kb on + strand at 1239.783 kb on + strand at 1239.824 kb on - strand at 1239.824 kb on - strand at 1239.952 kb on + strand at 1239.952 kb on + strand at 1240.096 kb on + strand at 1240.301 kb on + strand, within RR42_RS26220 at 1240.312 kb on + strand, within RR42_RS26220 at 1241.703 kb on + strand at 1241.703 kb on + strand at 1241.704 kb on - strand at 1241.796 kb on + strand, within RR42_RS26230 at 1241.796 kb on + strand, within RR42_RS26230 at 1241.796 kb on + strand, within RR42_RS26230 at 1241.797 kb on - strand, within RR42_RS26230 at 1241.797 kb on - strand, within RR42_RS26230 at 1241.868 kb on + strand, within RR42_RS26230 at 1241.868 kb on + strand, within RR42_RS26230 at 1241.869 kb on - strand, within RR42_RS26230 at 1241.901 kb on + strand, within RR42_RS26230 at 1241.901 kb on + strand, within RR42_RS26230 at 1241.902 kb on - strand, within RR42_RS26230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,239,781 + -1.0 1,239,781 + -0.5 1,239,781 + +1.0 1,239,781 + +0.0 1,239,781 + -0.0 1,239,781 + -0.6 1,239,781 + +1.3 1,239,782 - -0.5 1,239,783 + -1.7 1,239,783 + +0.6 1,239,824 - -0.4 1,239,824 - -2.0 1,239,952 + -0.9 1,239,952 + +0.5 1,240,096 + -0.4 1,240,301 + RR42_RS26220 0.32 -2.1 1,240,312 + RR42_RS26220 0.33 -1.8 1,241,703 + -0.2 1,241,703 + -2.2 1,241,704 - -0.2 1,241,796 + RR42_RS26230 0.12 +1.1 1,241,796 + RR42_RS26230 0.12 +0.3 1,241,796 + RR42_RS26230 0.12 +1.2 1,241,797 - RR42_RS26230 0.12 -0.9 1,241,797 - RR42_RS26230 0.12 +0.7 1,241,868 + RR42_RS26230 0.17 -2.7 1,241,868 + RR42_RS26230 0.17 +0.2 1,241,869 - RR42_RS26230 0.17 +1.1 1,241,901 + RR42_RS26230 0.20 +0.4 1,241,901 + RR42_RS26230 0.20 -1.5 1,241,902 - RR42_RS26230 0.20 +1.0
Or see this region's nucleotide sequence