Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25965 and RR42_RS25970 are separated by 124 nucleotides RR42_RS25970 and RR42_RS25975 are separated by 94 nucleotides RR42_RS25975 and RR42_RS25980 are separated by 220 nucleotides
RR42_RS25965: RR42_RS25965 - LuxR family transcriptional regulator, at 1,170,920 to 1,171,579
_RS25965
RR42_RS25970: RR42_RS25970 - acyl-CoA dehydrogenase, at 1,171,704 to 1,172,861
_RS25970
RR42_RS25975: RR42_RS25975 - hypothetical protein, at 1,172,956 to 1,173,195
_RS25975
RR42_RS25980: RR42_RS25980 - hypothetical protein, at 1,173,416 to 1,174,057
_RS25980
Position (kb)
1171
1172
1173 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1170.718 kb on + strand at 1170.721 kb on + strand at 1170.722 kb on - strand at 1170.759 kb on + strand at 1170.759 kb on + strand at 1170.759 kb on + strand at 1170.786 kb on + strand at 1170.787 kb on - strand at 1170.807 kb on + strand at 1170.808 kb on - strand at 1171.209 kb on - strand, within RR42_RS25965 at 1171.209 kb on - strand, within RR42_RS25965 at 1171.209 kb on - strand, within RR42_RS25965 at 1171.217 kb on - strand, within RR42_RS25965 at 1171.217 kb on - strand, within RR42_RS25965 at 1171.592 kb on - strand at 1171.605 kb on + strand at 1171.641 kb on + strand at 1171.641 kb on + strand at 1172.003 kb on + strand, within RR42_RS25970 at 1172.004 kb on - strand, within RR42_RS25970 at 1172.196 kb on - strand, within RR42_RS25970 at 1172.324 kb on + strand, within RR42_RS25970 at 1172.325 kb on - strand, within RR42_RS25970 at 1172.508 kb on + strand, within RR42_RS25970 at 1172.683 kb on - strand, within RR42_RS25970 at 1172.683 kb on - strand, within RR42_RS25970 at 1172.802 kb on + strand at 1172.802 kb on + strand at 1172.802 kb on + strand at 1172.803 kb on - strand at 1172.803 kb on - strand at 1172.939 kb on + strand at 1172.939 kb on + strand at 1172.940 kb on - strand at 1172.947 kb on - strand at 1173.263 kb on + strand at 1173.268 kb on + strand at 1173.269 kb on - strand at 1173.362 kb on + strand at 1173.362 kb on + strand at 1173.363 kb on - strand at 1173.363 kb on - strand at 1173.369 kb on + strand at 1173.369 kb on + strand at 1173.370 kb on - strand at 1173.437 kb on + strand at 1173.528 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,170,718 + -0.8 1,170,721 + -0.2 1,170,722 - -0.7 1,170,759 + -0.8 1,170,759 + -0.1 1,170,759 + -2.8 1,170,786 + +1.4 1,170,787 - -1.3 1,170,807 + +0.4 1,170,808 - -2.1 1,171,209 - RR42_RS25965 0.44 +0.1 1,171,209 - RR42_RS25965 0.44 -0.6 1,171,209 - RR42_RS25965 0.44 -1.7 1,171,217 - RR42_RS25965 0.45 +0.5 1,171,217 - RR42_RS25965 0.45 -1.0 1,171,592 - +0.1 1,171,605 + +0.6 1,171,641 + -0.4 1,171,641 + -2.8 1,172,003 + RR42_RS25970 0.26 +0.1 1,172,004 - RR42_RS25970 0.26 +0.5 1,172,196 - RR42_RS25970 0.42 -1.9 1,172,324 + RR42_RS25970 0.54 -0.5 1,172,325 - RR42_RS25970 0.54 +0.4 1,172,508 + RR42_RS25970 0.69 -1.3 1,172,683 - RR42_RS25970 0.85 -3.0 1,172,683 - RR42_RS25970 0.85 +0.1 1,172,802 + -0.2 1,172,802 + +0.7 1,172,802 + +0.3 1,172,803 - -1.2 1,172,803 - +0.9 1,172,939 + +0.9 1,172,939 + -1.9 1,172,940 - -1.1 1,172,947 - +0.4 1,173,263 + +1.2 1,173,268 + +0.6 1,173,269 - +0.1 1,173,362 + +1.0 1,173,362 + -0.2 1,173,363 - +0.2 1,173,363 - -0.9 1,173,369 + -0.5 1,173,369 + +1.3 1,173,370 - +1.5 1,173,437 + -1.5 1,173,528 + RR42_RS25980 0.17 +2.7 1,173,655 + RR42_RS25980 0.37 -0.5 1,173,655 + RR42_RS25980 0.37 -0.9 1,173,655 + RR42_RS25980 0.37 -2.1 1,173,655 + RR42_RS25980 0.37 +0.6
Or see this region's nucleotide sequence