Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25585

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25580 and RR42_RS25585 are separated by 237 nucleotidesRR42_RS25585 and RR42_RS25590 are separated by 499 nucleotides RR42_RS25580: RR42_RS25580 - haloacid dehalogenase, at 1,083,281 to 1,083,991 _RS25580 RR42_RS25585: RR42_RS25585 - membrane protein, at 1,084,229 to 1,085,392 _RS25585 RR42_RS25590: RR42_RS25590 - glucose 1-dehydrogenase, at 1,085,892 to 1,086,635 _RS25590 Position (kb) 1084 1085 1086Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1083.328 kb on - strandat 1083.350 kb on + strandat 1083.350 kb on + strandat 1083.351 kb on - strandat 1083.431 kb on + strand, within RR42_RS25580at 1083.432 kb on - strand, within RR42_RS25580at 1083.432 kb on - strand, within RR42_RS25580at 1083.668 kb on - strand, within RR42_RS25580at 1083.705 kb on + strand, within RR42_RS25580at 1083.746 kb on + strand, within RR42_RS25580at 1083.746 kb on + strand, within RR42_RS25580at 1083.747 kb on - strand, within RR42_RS25580at 1083.875 kb on + strand, within RR42_RS25580at 1083.875 kb on + strand, within RR42_RS25580at 1083.876 kb on - strand, within RR42_RS25580at 1083.876 kb on - strand, within RR42_RS25580at 1084.116 kb on + strandat 1084.116 kb on + strandat 1084.117 kb on - strandat 1084.131 kb on + strandat 1084.199 kb on + strandat 1084.307 kb on + strandat 1084.308 kb on - strandat 1084.335 kb on - strandat 1084.453 kb on + strand, within RR42_RS25585at 1084.453 kb on + strand, within RR42_RS25585at 1084.728 kb on - strand, within RR42_RS25585at 1084.750 kb on + strand, within RR42_RS25585at 1084.887 kb on - strand, within RR42_RS25585at 1084.920 kb on - strand, within RR42_RS25585at 1084.920 kb on - strand, within RR42_RS25585at 1084.989 kb on - strand, within RR42_RS25585at 1084.989 kb on - strand, within RR42_RS25585at 1084.995 kb on - strand, within RR42_RS25585at 1085.075 kb on + strand, within RR42_RS25585at 1085.076 kb on - strand, within RR42_RS25585at 1085.078 kb on + strand, within RR42_RS25585at 1085.097 kb on - strand, within RR42_RS25585at 1085.133 kb on - strand, within RR42_RS25585at 1085.138 kb on + strand, within RR42_RS25585at 1085.139 kb on - strand, within RR42_RS25585at 1085.210 kb on + strand, within RR42_RS25585at 1085.227 kb on + strand, within RR42_RS25585at 1085.237 kb on + strand, within RR42_RS25585at 1085.238 kb on - strand, within RR42_RS25585at 1085.328 kb on - strandat 1085.447 kb on - strandat 1085.447 kb on - strandat 1085.612 kb on - strandat 1085.683 kb on + strandat 1085.723 kb on - strandat 1085.969 kb on - strand, within RR42_RS25590at 1085.989 kb on + strand, within RR42_RS25590at 1085.990 kb on - strand, within RR42_RS25590at 1085.990 kb on - strand, within RR42_RS25590at 1086.281 kb on - strand, within RR42_RS25590at 1086.281 kb on - strand, within RR42_RS25590

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,083,328 - +0.6
1,083,350 + +0.5
1,083,350 + -0.9
1,083,351 - -0.9
1,083,431 + RR42_RS25580 0.21 -0.2
1,083,432 - RR42_RS25580 0.21 -2.3
1,083,432 - RR42_RS25580 0.21 -2.7
1,083,668 - RR42_RS25580 0.54 -0.1
1,083,705 + RR42_RS25580 0.60 -1.9
1,083,746 + RR42_RS25580 0.65 +0.6
1,083,746 + RR42_RS25580 0.65 +1.0
1,083,747 - RR42_RS25580 0.66 +0.2
1,083,875 + RR42_RS25580 0.84 +0.8
1,083,875 + RR42_RS25580 0.84 -0.6
1,083,876 - RR42_RS25580 0.84 +1.4
1,083,876 - RR42_RS25580 0.84 -1.7
1,084,116 + -0.7
1,084,116 + -0.3
1,084,117 - -0.9
1,084,131 + +0.8
1,084,199 + +0.3
1,084,307 + +0.7
1,084,308 - +0.4
1,084,335 - -0.3
1,084,453 + RR42_RS25585 0.19 +0.2
1,084,453 + RR42_RS25585 0.19 -2.4
1,084,728 - RR42_RS25585 0.43 +1.2
1,084,750 + RR42_RS25585 0.45 +0.7
1,084,887 - RR42_RS25585 0.57 +0.5
1,084,920 - RR42_RS25585 0.59 -2.8
1,084,920 - RR42_RS25585 0.59 +0.6
1,084,989 - RR42_RS25585 0.65 +0.8
1,084,989 - RR42_RS25585 0.65 -0.5
1,084,995 - RR42_RS25585 0.66 +0.6
1,085,075 + RR42_RS25585 0.73 -0.0
1,085,076 - RR42_RS25585 0.73 +2.5
1,085,078 + RR42_RS25585 0.73 -0.8
1,085,097 - RR42_RS25585 0.75 -0.5
1,085,133 - RR42_RS25585 0.78 -1.5
1,085,138 + RR42_RS25585 0.78 -0.6
1,085,139 - RR42_RS25585 0.78 -0.8
1,085,210 + RR42_RS25585 0.84 +0.1
1,085,227 + RR42_RS25585 0.86 +0.0
1,085,237 + RR42_RS25585 0.87 +0.9
1,085,238 - RR42_RS25585 0.87 -2.0
1,085,328 - -0.7
1,085,447 - +0.7
1,085,447 - -0.3
1,085,612 - +0.7
1,085,683 + -0.2
1,085,723 - -1.9
1,085,969 - RR42_RS25590 0.10 -0.5
1,085,989 + RR42_RS25590 0.13 +0.5
1,085,990 - RR42_RS25590 0.13 -0.3
1,085,990 - RR42_RS25590 0.13 -1.5
1,086,281 - RR42_RS25590 0.52 +0.4
1,086,281 - RR42_RS25590 0.52 +0.3

Or see this region's nucleotide sequence