Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25565 and RR42_RS25570 are separated by 1 nucleotides RR42_RS25570 and RR42_RS25575 are separated by 45 nucleotides RR42_RS25575 and RR42_RS25580 are separated by 79 nucleotides
RR42_RS25565: RR42_RS25565 - alpha/beta hydrolase, at 1,079,565 to 1,080,719
_RS25565
RR42_RS25570: RR42_RS25570 - long-chain fatty acid--CoA ligase, at 1,080,721 to 1,082,400
_RS25570
RR42_RS25575: RR42_RS25575 - aldolase, at 1,082,446 to 1,083,201
_RS25575
RR42_RS25580: RR42_RS25580 - haloacid dehalogenase, at 1,083,281 to 1,083,991
_RS25580
Position (kb)
1080
1081
1082
1083 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1079.772 kb on + strand, within RR42_RS25565 at 1079.772 kb on + strand, within RR42_RS25565 at 1079.773 kb on - strand, within RR42_RS25565 at 1079.879 kb on - strand, within RR42_RS25565 at 1080.139 kb on - strand, within RR42_RS25565 at 1080.139 kb on - strand, within RR42_RS25565 at 1080.139 kb on - strand, within RR42_RS25565 at 1080.142 kb on - strand, within RR42_RS25565 at 1080.142 kb on - strand, within RR42_RS25565 at 1080.211 kb on - strand, within RR42_RS25565 at 1080.213 kb on + strand, within RR42_RS25565 at 1080.214 kb on - strand, within RR42_RS25565 at 1080.300 kb on + strand, within RR42_RS25565 at 1080.301 kb on - strand, within RR42_RS25565 at 1080.303 kb on + strand, within RR42_RS25565 at 1080.408 kb on + strand, within RR42_RS25565 at 1080.410 kb on + strand, within RR42_RS25565 at 1080.457 kb on - strand, within RR42_RS25565 at 1080.721 kb on - strand at 1080.721 kb on - strand at 1080.838 kb on + strand at 1080.862 kb on + strand at 1080.968 kb on - strand, within RR42_RS25570 at 1081.014 kb on + strand, within RR42_RS25570 at 1081.015 kb on - strand, within RR42_RS25570 at 1081.015 kb on - strand, within RR42_RS25570 at 1081.210 kb on + strand, within RR42_RS25570 at 1081.288 kb on + strand, within RR42_RS25570 at 1081.289 kb on - strand, within RR42_RS25570 at 1081.289 kb on - strand, within RR42_RS25570 at 1081.353 kb on + strand, within RR42_RS25570 at 1081.376 kb on + strand, within RR42_RS25570 at 1081.417 kb on + strand, within RR42_RS25570 at 1081.417 kb on + strand, within RR42_RS25570 at 1081.417 kb on + strand, within RR42_RS25570 at 1081.465 kb on + strand, within RR42_RS25570 at 1081.465 kb on + strand, within RR42_RS25570 at 1081.848 kb on + strand, within RR42_RS25570 at 1081.936 kb on + strand, within RR42_RS25570 at 1081.936 kb on + strand, within RR42_RS25570 at 1082.211 kb on - strand, within RR42_RS25570 at 1082.234 kb on - strand at 1082.234 kb on - strand at 1082.429 kb on - strand at 1082.429 kb on - strand at 1082.433 kb on - strand at 1082.449 kb on + strand at 1082.449 kb on + strand at 1082.449 kb on + strand at 1082.450 kb on - strand at 1082.450 kb on - strand at 1082.778 kb on + strand, within RR42_RS25575 at 1082.847 kb on - strand, within RR42_RS25575 at 1082.847 kb on - strand, within RR42_RS25575 at 1082.867 kb on - strand, within RR42_RS25575 at 1082.875 kb on + strand, within RR42_RS25575 at 1082.876 kb on - strand, within RR42_RS25575 at 1082.876 kb on - strand, within RR42_RS25575 at 1082.883 kb on + strand, within RR42_RS25575 at 1082.883 kb on + strand, within RR42_RS25575 at 1082.942 kb on - strand, within RR42_RS25575 at 1082.976 kb on - strand, within RR42_RS25575 at 1082.976 kb on - strand, within RR42_RS25575 at 1082.976 kb on - strand, within RR42_RS25575 at 1083.010 kb on + strand, within RR42_RS25575 at 1083.010 kb on + strand, within RR42_RS25575 at 1083.011 kb on - strand, within RR42_RS25575 at 1083.177 kb on + strand at 1083.328 kb on - strand at 1083.350 kb on + strand at 1083.350 kb on + strand at 1083.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,079,772 + RR42_RS25565 0.18 -1.1 1,079,772 + RR42_RS25565 0.18 +0.1 1,079,773 - RR42_RS25565 0.18 -1.7 1,079,879 - RR42_RS25565 0.27 -0.1 1,080,139 - RR42_RS25565 0.50 -0.7 1,080,139 - RR42_RS25565 0.50 +0.3 1,080,139 - RR42_RS25565 0.50 +0.1 1,080,142 - RR42_RS25565 0.50 -0.9 1,080,142 - RR42_RS25565 0.50 -1.1 1,080,211 - RR42_RS25565 0.56 +0.1 1,080,213 + RR42_RS25565 0.56 -0.1 1,080,214 - RR42_RS25565 0.56 +4.4 1,080,300 + RR42_RS25565 0.64 -2.1 1,080,301 - RR42_RS25565 0.64 +2.0 1,080,303 + RR42_RS25565 0.64 -0.7 1,080,408 + RR42_RS25565 0.73 +0.3 1,080,410 + RR42_RS25565 0.73 -0.3 1,080,457 - RR42_RS25565 0.77 -1.7 1,080,721 - +0.9 1,080,721 - -0.1 1,080,838 + -3.8 1,080,862 + +0.3 1,080,968 - RR42_RS25570 0.15 +1.9 1,081,014 + RR42_RS25570 0.17 +1.3 1,081,015 - RR42_RS25570 0.17 +1.5 1,081,015 - RR42_RS25570 0.17 +0.1 1,081,210 + RR42_RS25570 0.29 +0.1 1,081,288 + RR42_RS25570 0.34 -0.8 1,081,289 - RR42_RS25570 0.34 +0.2 1,081,289 - RR42_RS25570 0.34 -0.0 1,081,353 + RR42_RS25570 0.38 -1.9 1,081,376 + RR42_RS25570 0.39 +0.6 1,081,417 + RR42_RS25570 0.41 -0.7 1,081,417 + RR42_RS25570 0.41 -1.5 1,081,417 + RR42_RS25570 0.41 +0.8 1,081,465 + RR42_RS25570 0.44 -2.1 1,081,465 + RR42_RS25570 0.44 -0.9 1,081,848 + RR42_RS25570 0.67 +1.0 1,081,936 + RR42_RS25570 0.72 +0.8 1,081,936 + RR42_RS25570 0.72 +0.0 1,082,211 - RR42_RS25570 0.89 +0.1 1,082,234 - +1.4 1,082,234 - -0.1 1,082,429 - -2.3 1,082,429 - -0.8 1,082,433 - -0.1 1,082,449 + -1.1 1,082,449 + -2.5 1,082,449 + -0.7 1,082,450 - -0.3 1,082,450 - +0.1 1,082,778 + RR42_RS25575 0.44 -0.6 1,082,847 - RR42_RS25575 0.53 +0.7 1,082,847 - RR42_RS25575 0.53 +0.4 1,082,867 - RR42_RS25575 0.56 -1.0 1,082,875 + RR42_RS25575 0.57 -2.1 1,082,876 - RR42_RS25575 0.57 -0.9 1,082,876 - RR42_RS25575 0.57 -2.5 1,082,883 + RR42_RS25575 0.58 -1.9 1,082,883 + RR42_RS25575 0.58 +2.7 1,082,942 - RR42_RS25575 0.66 +0.0 1,082,976 - RR42_RS25575 0.70 -2.3 1,082,976 - RR42_RS25575 0.70 +0.1 1,082,976 - RR42_RS25575 0.70 -0.6 1,083,010 + RR42_RS25575 0.75 +1.1 1,083,010 + RR42_RS25575 0.75 -1.2 1,083,011 - RR42_RS25575 0.75 +0.7 1,083,177 + +0.6 1,083,328 - +0.6 1,083,350 + +0.5 1,083,350 + -0.9 1,083,351 - -0.9
Or see this region's nucleotide sequence