Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25250

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25240 and RR42_RS25245 are separated by 164 nucleotidesRR42_RS25245 and RR42_RS25250 are separated by 27 nucleotidesRR42_RS25250 and RR42_RS25255 are separated by 106 nucleotides RR42_RS25240: RR42_RS25240 - nitronate monooxygenase, at 1,003,381 to 1,004,388 _RS25240 RR42_RS25245: RR42_RS25245 - Asp/Glu/hydantoin racemase, at 1,004,553 to 1,005,209 _RS25245 RR42_RS25250: RR42_RS25250 - ABC transporter substrate-binding protein, at 1,005,237 to 1,006,223 _RS25250 RR42_RS25255: RR42_RS25255 - 1,4-butanediol diacrylate esterase, at 1,006,330 to 1,007,493 _RS25255 Position (kb) 1005 1006 1007Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1004.402 kb on - strandat 1004.463 kb on - strandat 1004.463 kb on - strandat 1004.496 kb on - strandat 1004.635 kb on + strand, within RR42_RS25245at 1004.635 kb on + strand, within RR42_RS25245at 1004.635 kb on + strand, within RR42_RS25245at 1004.636 kb on - strand, within RR42_RS25245at 1004.636 kb on - strand, within RR42_RS25245at 1004.758 kb on - strand, within RR42_RS25245at 1004.999 kb on + strand, within RR42_RS25245at 1004.999 kb on + strand, within RR42_RS25245at 1005.000 kb on - strand, within RR42_RS25245at 1005.043 kb on + strand, within RR42_RS25245at 1005.043 kb on + strand, within RR42_RS25245at 1005.184 kb on + strandat 1005.254 kb on + strandat 1005.255 kb on - strandat 1005.255 kb on - strandat 1005.255 kb on - strandat 1005.291 kb on + strandat 1005.291 kb on + strandat 1005.407 kb on + strand, within RR42_RS25250at 1005.408 kb on - strand, within RR42_RS25250at 1005.408 kb on - strand, within RR42_RS25250at 1005.668 kb on - strand, within RR42_RS25250at 1005.892 kb on + strand, within RR42_RS25250at 1005.892 kb on + strand, within RR42_RS25250at 1005.893 kb on - strand, within RR42_RS25250at 1005.927 kb on + strand, within RR42_RS25250at 1005.959 kb on - strand, within RR42_RS25250at 1006.141 kb on + strandat 1006.283 kb on - strandat 1006.367 kb on + strandat 1006.457 kb on + strand, within RR42_RS25255at 1006.458 kb on - strand, within RR42_RS25255at 1006.461 kb on + strand, within RR42_RS25255at 1006.461 kb on + strand, within RR42_RS25255at 1006.745 kb on + strand, within RR42_RS25255at 1006.851 kb on + strand, within RR42_RS25255at 1006.909 kb on - strand, within RR42_RS25255at 1006.923 kb on - strand, within RR42_RS25255at 1006.923 kb on - strand, within RR42_RS25255at 1007.078 kb on + strand, within RR42_RS25255at 1007.111 kb on + strand, within RR42_RS25255at 1007.111 kb on + strand, within RR42_RS25255at 1007.112 kb on - strand, within RR42_RS25255

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,004,402 - +0.9
1,004,463 - +0.7
1,004,463 - +0.4
1,004,496 - +0.9
1,004,635 + RR42_RS25245 0.12 -0.4
1,004,635 + RR42_RS25245 0.12 +0.4
1,004,635 + RR42_RS25245 0.12 -1.9
1,004,636 - RR42_RS25245 0.13 -0.4
1,004,636 - RR42_RS25245 0.13 -0.7
1,004,758 - RR42_RS25245 0.31 -0.1
1,004,999 + RR42_RS25245 0.68 +0.6
1,004,999 + RR42_RS25245 0.68 +0.1
1,005,000 - RR42_RS25245 0.68 -0.6
1,005,043 + RR42_RS25245 0.75 -2.2
1,005,043 + RR42_RS25245 0.75 -0.1
1,005,184 + +0.2
1,005,254 + +0.7
1,005,255 - +0.7
1,005,255 - +0.3
1,005,255 - +1.3
1,005,291 + +0.3
1,005,291 + +0.7
1,005,407 + RR42_RS25250 0.17 -0.4
1,005,408 - RR42_RS25250 0.17 -3.2
1,005,408 - RR42_RS25250 0.17 -0.8
1,005,668 - RR42_RS25250 0.44 +1.9
1,005,892 + RR42_RS25250 0.66 +0.2
1,005,892 + RR42_RS25250 0.66 -0.3
1,005,893 - RR42_RS25250 0.66 -0.6
1,005,927 + RR42_RS25250 0.70 +1.1
1,005,959 - RR42_RS25250 0.73 -0.3
1,006,141 + +0.6
1,006,283 - -1.1
1,006,367 + +0.4
1,006,457 + RR42_RS25255 0.11 -1.9
1,006,458 - RR42_RS25255 0.11 -0.2
1,006,461 + RR42_RS25255 0.11 -0.2
1,006,461 + RR42_RS25255 0.11 +0.3
1,006,745 + RR42_RS25255 0.36 -0.8
1,006,851 + RR42_RS25255 0.45 +0.8
1,006,909 - RR42_RS25255 0.50 -1.7
1,006,923 - RR42_RS25255 0.51 -0.5
1,006,923 - RR42_RS25255 0.51 +1.3
1,007,078 + RR42_RS25255 0.64 +1.2
1,007,111 + RR42_RS25255 0.67 +0.6
1,007,111 + RR42_RS25255 0.67 -1.6
1,007,112 - RR42_RS25255 0.67 +0.1

Or see this region's nucleotide sequence