Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25225

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25220 and RR42_RS25225 are separated by 10 nucleotidesRR42_RS25225 and RR42_RS25230 overlap by 1 nucleotides RR42_RS25220: RR42_RS25220 - NADP transhydrogenase subunit alpha, at 999,456 to 1,000,574 _RS25220 RR42_RS25225: RR42_RS25225 - NAD(P) transhydrogenase, at 1,000,585 to 1,000,908 _RS25225 RR42_RS25230: RR42_RS25230 - NAD synthetase, at 1,000,908 to 1,002,377 _RS25230 Position (kb) 1000 1001Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 999.765 kb on - strand, within RR42_RS25220at 999.765 kb on - strand, within RR42_RS25220at 999.838 kb on + strand, within RR42_RS25220at 999.838 kb on + strand, within RR42_RS25220at 999.839 kb on - strand, within RR42_RS25220at 999.839 kb on - strand, within RR42_RS25220at 999.839 kb on - strand, within RR42_RS25220at 999.839 kb on - strand, within RR42_RS25220at 999.897 kb on + strand, within RR42_RS25220at 999.898 kb on - strand, within RR42_RS25220at 1000.597 kb on - strandat 1000.639 kb on + strand, within RR42_RS25225at 1000.640 kb on - strand, within RR42_RS25225at 1000.881 kb on + strandat 1000.882 kb on - strandat 1000.944 kb on + strandat 1000.944 kb on + strandat 1001.185 kb on + strand, within RR42_RS25230at 1001.428 kb on - strand, within RR42_RS25230at 1001.652 kb on + strand, within RR42_RS25230at 1001.653 kb on - strand, within RR42_RS25230at 1001.752 kb on - strand, within RR42_RS25230at 1001.752 kb on - strand, within RR42_RS25230at 1001.752 kb on - strand, within RR42_RS25230

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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999,765 - RR42_RS25220 0.28 -1.9
999,765 - RR42_RS25220 0.28 +0.3
999,838 + RR42_RS25220 0.34 +0.2
999,838 + RR42_RS25220 0.34 -0.5
999,839 - RR42_RS25220 0.34 +1.1
999,839 - RR42_RS25220 0.34 -0.9
999,839 - RR42_RS25220 0.34 -0.3
999,839 - RR42_RS25220 0.34 -1.9
999,897 + RR42_RS25220 0.39 +1.9
999,898 - RR42_RS25220 0.39 +0.0
1,000,597 - -2.8
1,000,639 + RR42_RS25225 0.17 -0.3
1,000,640 - RR42_RS25225 0.17 +1.0
1,000,881 + -0.5
1,000,882 - -0.9
1,000,944 + -2.5
1,000,944 + +0.6
1,001,185 + RR42_RS25230 0.19 -0.2
1,001,428 - RR42_RS25230 0.35 -1.4
1,001,652 + RR42_RS25230 0.51 -2.3
1,001,653 - RR42_RS25230 0.51 +0.6
1,001,752 - RR42_RS25230 0.57 +1.1
1,001,752 - RR42_RS25230 0.57 -0.8
1,001,752 - RR42_RS25230 0.57 +1.5

Or see this region's nucleotide sequence