Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24280 and RR42_RS24285 are separated by 1 nucleotides
RR42_RS24280: RR42_RS24280 - gentisate 1,2-dioxygenase, at 774,475 to 775,524
_RS24280
RR42_RS24285: RR42_RS24285 - 2-polyprenyl-6-methoxyphenol hydroxylase, at 775,526 to 777,196
_RS24285
Position (kb)
775
776
777
778 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 774.668 kb on + strand, within RR42_RS24280 at 774.669 kb on - strand, within RR42_RS24280 at 774.671 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.863 kb on + strand, within RR42_RS24280 at 775.257 kb on + strand, within RR42_RS24280 at 775.257 kb on + strand, within RR42_RS24280 at 775.458 kb on - strand at 775.524 kb on + strand at 775.524 kb on + strand at 775.524 kb on + strand at 775.525 kb on - strand at 775.525 kb on - strand at 775.545 kb on + strand at 775.661 kb on + strand at 775.661 kb on + strand at 775.683 kb on + strand at 775.683 kb on + strand at 775.684 kb on - strand at 775.684 kb on - strand at 775.684 kb on - strand at 775.684 kb on - strand at 775.799 kb on + strand, within RR42_RS24285 at 775.799 kb on + strand, within RR42_RS24285 at 775.800 kb on - strand, within RR42_RS24285 at 775.800 kb on - strand, within RR42_RS24285 at 775.803 kb on + strand, within RR42_RS24285 at 775.803 kb on + strand, within RR42_RS24285 at 775.823 kb on + strand, within RR42_RS24285 at 775.953 kb on + strand, within RR42_RS24285 at 775.960 kb on - strand, within RR42_RS24285 at 776.008 kb on + strand, within RR42_RS24285 at 776.008 kb on + strand, within RR42_RS24285 at 776.008 kb on + strand, within RR42_RS24285 at 776.009 kb on - strand, within RR42_RS24285 at 776.009 kb on - strand, within RR42_RS24285 at 776.260 kb on + strand, within RR42_RS24285 at 776.261 kb on - strand, within RR42_RS24285 at 776.462 kb on + strand, within RR42_RS24285 at 776.462 kb on + strand, within RR42_RS24285 at 776.539 kb on + strand, within RR42_RS24285 at 776.539 kb on + strand, within RR42_RS24285 at 776.540 kb on - strand, within RR42_RS24285 at 776.540 kb on - strand, within RR42_RS24285 at 776.540 kb on - strand, within RR42_RS24285 at 776.540 kb on - strand, within RR42_RS24285 at 776.540 kb on - strand, within RR42_RS24285 at 776.542 kb on - strand, within RR42_RS24285 at 776.605 kb on + strand, within RR42_RS24285 at 776.605 kb on + strand, within RR42_RS24285 at 776.605 kb on + strand, within RR42_RS24285 at 776.606 kb on - strand, within RR42_RS24285 at 776.606 kb on - strand, within RR42_RS24285 at 776.610 kb on + strand, within RR42_RS24285 at 776.610 kb on + strand, within RR42_RS24285 at 776.611 kb on - strand, within RR42_RS24285 at 776.611 kb on - strand, within RR42_RS24285 at 776.618 kb on + strand, within RR42_RS24285 at 776.747 kb on + strand, within RR42_RS24285 at 776.747 kb on + strand, within RR42_RS24285 at 776.748 kb on - strand, within RR42_RS24285 at 776.790 kb on + strand, within RR42_RS24285 at 776.937 kb on + strand, within RR42_RS24285 at 776.937 kb on + strand, within RR42_RS24285 at 776.989 kb on - strand, within RR42_RS24285 at 777.257 kb on + strand at 777.258 kb on - strand at 777.300 kb on - strand at 777.303 kb on - strand at 777.360 kb on - strand at 777.380 kb on + strand at 777.381 kb on - strand at 777.430 kb on + strand at 777.431 kb on - strand at 777.431 kb on - strand at 777.444 kb on + strand at 777.444 kb on + strand at 777.444 kb on + strand at 777.445 kb on - strand at 777.549 kb on + strand at 777.550 kb on - strand at 777.586 kb on + strand at 777.586 kb on + strand at 777.587 kb on - strand at 777.587 kb on - strand at 777.595 kb on + strand at 777.697 kb on - strand at 777.761 kb on + strand at 777.762 kb on - strand at 778.004 kb on + strand at 778.004 kb on + strand at 778.004 kb on + strand at 778.004 kb on + strand at 778.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 774,668 + RR42_RS24280 0.18 -0.4 774,669 - RR42_RS24280 0.18 -0.5 774,671 + RR42_RS24280 0.19 +0.0 774,803 + RR42_RS24280 0.31 +1.5 774,803 + RR42_RS24280 0.31 +0.9 774,803 + RR42_RS24280 0.31 -0.9 774,803 + RR42_RS24280 0.31 -2.5 774,863 + RR42_RS24280 0.37 +0.8 775,257 + RR42_RS24280 0.74 -0.9 775,257 + RR42_RS24280 0.74 -1.2 775,458 - +0.2 775,524 + +0.0 775,524 + +1.2 775,524 + +0.2 775,525 - -0.1 775,525 - -3.2 775,545 + -1.8 775,661 + +0.3 775,661 + +0.5 775,683 + -1.1 775,683 + -2.0 775,684 - -1.3 775,684 - -1.1 775,684 - +0.2 775,684 - +1.5 775,799 + RR42_RS24285 0.16 -0.0 775,799 + RR42_RS24285 0.16 +0.0 775,800 - RR42_RS24285 0.16 -1.6 775,800 - RR42_RS24285 0.16 -0.5 775,803 + RR42_RS24285 0.17 +1.1 775,803 + RR42_RS24285 0.17 -2.5 775,823 + RR42_RS24285 0.18 +0.6 775,953 + RR42_RS24285 0.26 +1.3 775,960 - RR42_RS24285 0.26 +0.5 776,008 + RR42_RS24285 0.29 +0.1 776,008 + RR42_RS24285 0.29 +0.5 776,008 + RR42_RS24285 0.29 -1.3 776,009 - RR42_RS24285 0.29 -2.5 776,009 - RR42_RS24285 0.29 +1.2 776,260 + RR42_RS24285 0.44 +0.3 776,261 - RR42_RS24285 0.44 -0.6 776,462 + RR42_RS24285 0.56 -1.3 776,462 + RR42_RS24285 0.56 +0.1 776,539 + RR42_RS24285 0.61 +0.7 776,539 + RR42_RS24285 0.61 +1.3 776,540 - RR42_RS24285 0.61 +0.2 776,540 - RR42_RS24285 0.61 -0.4 776,540 - RR42_RS24285 0.61 -1.1 776,540 - RR42_RS24285 0.61 +0.8 776,540 - RR42_RS24285 0.61 -0.6 776,542 - RR42_RS24285 0.61 -0.6 776,605 + RR42_RS24285 0.65 -1.4 776,605 + RR42_RS24285 0.65 +1.2 776,605 + RR42_RS24285 0.65 -1.5 776,606 - RR42_RS24285 0.65 -0.4 776,606 - RR42_RS24285 0.65 -1.6 776,610 + RR42_RS24285 0.65 -2.6 776,610 + RR42_RS24285 0.65 -1.9 776,611 - RR42_RS24285 0.65 -0.6 776,611 - RR42_RS24285 0.65 -0.3 776,618 + RR42_RS24285 0.65 -0.1 776,747 + RR42_RS24285 0.73 -0.7 776,747 + RR42_RS24285 0.73 -0.7 776,748 - RR42_RS24285 0.73 +0.5 776,790 + RR42_RS24285 0.76 +1.1 776,937 + RR42_RS24285 0.84 +0.3 776,937 + RR42_RS24285 0.84 -0.8 776,989 - RR42_RS24285 0.88 -1.6 777,257 + -3.6 777,258 - +0.1 777,300 - -0.1 777,303 - +0.0 777,360 - -1.7 777,380 + -0.1 777,381 - -1.6 777,430 + +0.6 777,431 - -1.2 777,431 - +0.5 777,444 + +1.1 777,444 + +1.8 777,444 + +0.0 777,445 - +1.0 777,549 + -0.7 777,550 - -0.8 777,586 + -0.6 777,586 + +0.0 777,587 - +0.3 777,587 - -0.5 777,595 + -1.6 777,697 - -0.4 777,761 + -3.9 777,762 - -1.0 778,004 + -0.6 778,004 + -1.0 778,004 + +0.6 778,004 + +0.2 778,005 - -0.6
Or see this region's nucleotide sequence