Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24270 and RR42_RS24275 are separated by 108 nucleotides RR42_RS24275 and RR42_RS24280 overlap by 4 nucleotides
RR42_RS24270: RR42_RS24270 - hypothetical protein, at 772,514 to 773,482
_RS24270
RR42_RS24275: RR42_RS24275 - hypothetical protein, at 773,591 to 774,478
_RS24275
RR42_RS24280: RR42_RS24280 - gentisate 1,2-dioxygenase, at 774,475 to 775,524
_RS24280
Position (kb) 773
774
775 Strain fitness (log2 ratio) -2
-1
0
1
2 at 772.755 kb on + strand, within RR42_RS24270 at 772.853 kb on - strand, within RR42_RS24270 at 772.938 kb on + strand, within RR42_RS24270 at 772.938 kb on + strand, within RR42_RS24270 at 772.939 kb on - strand at 773.164 kb on - strand, within RR42_RS24270 at 773.164 kb on - strand, within RR42_RS24270 at 773.164 kb on - strand, within RR42_RS24270 at 773.230 kb on - strand, within RR42_RS24270 at 773.469 kb on - strand at 773.504 kb on - strand at 773.592 kb on + strand at 773.593 kb on - strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.596 kb on - strand at 773.675 kb on - strand at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.835 kb on - strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.401 kb on + strand at 774.402 kb on - strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.421 kb on - strand at 774.421 kb on - strand at 774.493 kb on - strand at 774.668 kb on + strand, within RR42_RS24280 at 774.669 kb on - strand, within RR42_RS24280 at 774.671 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.863 kb on + strand, within RR42_RS24280 at 775.257 kb on + strand, within RR42_RS24280 at 775.257 kb on + strand, within RR42_RS24280 at 775.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 772,755 + RR42_RS24270 0.25 -0.4 772,853 - RR42_RS24270 0.35 +0.5 772,938 + RR42_RS24270 0.44 -0.1 772,938 + RR42_RS24270 0.44 +0.4 772,939 - -1.0 773,164 - RR42_RS24270 0.67 +0.7 773,164 - RR42_RS24270 0.67 +0.2 773,164 - RR42_RS24270 0.67 -0.1 773,230 - RR42_RS24270 0.74 +1.0 773,469 - +0.1 773,504 - +0.1 773,592 + +1.0 773,593 - +0.3 773,595 + -0.1 773,595 + +1.3 773,595 + +0.1 773,596 - +0.2 773,675 - +1.7 773,834 + RR42_RS24275 0.27 -0.1 773,834 + RR42_RS24275 0.27 -2.3 773,834 + RR42_RS24275 0.27 +1.7 773,835 - RR42_RS24275 0.27 +0.3 774,000 + RR42_RS24275 0.46 +1.5 774,000 + RR42_RS24275 0.46 -0.9 774,243 - RR42_RS24275 0.73 +2.6 774,243 - RR42_RS24275 0.73 -0.4 774,243 - RR42_RS24275 0.73 +1.3 774,243 - RR42_RS24275 0.73 +1.4 774,401 + +0.4 774,402 - +0.6 774,420 + +0.5 774,420 + +1.0 774,420 + -1.8 774,421 - -1.8 774,421 - +0.0 774,493 - -0.4 774,668 + RR42_RS24280 0.18 -0.4 774,669 - RR42_RS24280 0.18 -0.5 774,671 + RR42_RS24280 0.19 +0.0 774,803 + RR42_RS24280 0.31 +1.5 774,803 + RR42_RS24280 0.31 +0.9 774,803 + RR42_RS24280 0.31 -0.9 774,803 + RR42_RS24280 0.31 -2.5 774,863 + RR42_RS24280 0.37 +0.8 775,257 + RR42_RS24280 0.74 -0.9 775,257 + RR42_RS24280 0.74 -1.2 775,458 - +0.2
Or see this region's nucleotide sequence