Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24170 and RR42_RS24175 are separated by 239 nucleotides RR42_RS24175 and RR42_RS24180 are separated by 29 nucleotides
RR42_RS24170: RR42_RS24170 - hypothetical protein, at 748,190 to 749,047
_RS24170
RR42_RS24175: RR42_RS24175 - isopropylmalate isomerase, at 749,287 to 750,696
_RS24175
RR42_RS24180: RR42_RS24180 - 3-isopropylmalate dehydratase, at 750,726 to 751,376
_RS24180
Position (kb)
749
750
751 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 748.295 kb on + strand, within RR42_RS24170 at 748.304 kb on + strand, within RR42_RS24170 at 748.304 kb on + strand, within RR42_RS24170 at 748.359 kb on + strand, within RR42_RS24170 at 748.449 kb on - strand, within RR42_RS24170 at 748.492 kb on - strand, within RR42_RS24170 at 748.754 kb on - strand, within RR42_RS24170 at 748.816 kb on - strand, within RR42_RS24170 at 748.816 kb on - strand, within RR42_RS24170 at 748.816 kb on - strand, within RR42_RS24170 at 748.877 kb on + strand, within RR42_RS24170 at 748.877 kb on + strand, within RR42_RS24170 at 748.878 kb on - strand, within RR42_RS24170 at 748.878 kb on - strand, within RR42_RS24170 at 748.878 kb on - strand, within RR42_RS24170 at 748.916 kb on + strand, within RR42_RS24170 at 748.916 kb on + strand, within RR42_RS24170 at 748.916 kb on + strand, within RR42_RS24170 at 748.938 kb on + strand, within RR42_RS24170 at 748.939 kb on - strand, within RR42_RS24170 at 748.979 kb on + strand at 748.979 kb on + strand at 749.045 kb on + strand at 749.046 kb on - strand at 749.303 kb on - strand at 749.362 kb on + strand at 749.362 kb on + strand at 749.362 kb on + strand at 749.363 kb on - strand at 749.363 kb on - strand at 749.677 kb on - strand, within RR42_RS24175 at 750.010 kb on + strand, within RR42_RS24175 at 750.044 kb on + strand, within RR42_RS24175 at 750.045 kb on - strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.340 kb on - strand, within RR42_RS24175 at 750.340 kb on - strand, within RR42_RS24175 at 750.586 kb on - strand at 750.586 kb on - strand at 750.659 kb on + strand at 750.659 kb on + strand at 750.660 kb on - strand at 750.660 kb on - strand at 750.702 kb on + strand at 750.703 kb on - strand at 750.706 kb on + strand at 750.706 kb on + strand at 750.707 kb on - strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.931 kb on + strand, within RR42_RS24180 at 751.087 kb on - strand, within RR42_RS24180 at 751.167 kb on + strand, within RR42_RS24180 at 751.167 kb on + strand, within RR42_RS24180 at 751.168 kb on - strand, within RR42_RS24180 at 751.225 kb on - strand, within RR42_RS24180 at 751.254 kb on + strand, within RR42_RS24180 at 751.255 kb on - strand, within RR42_RS24180 at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.380 kb on - strand at 751.457 kb on + strand at 751.458 kb on - strand at 751.472 kb on + strand at 751.499 kb on + strand at 751.521 kb on - strand at 751.557 kb on + strand at 751.558 kb on - strand at 751.601 kb on + strand at 751.617 kb on + strand at 751.618 kb on - strand at 751.630 kb on + strand at 751.630 kb on + strand at 751.669 kb on + strand at 751.669 kb on + strand at 751.692 kb on + strand at 751.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 748,295 + RR42_RS24170 0.12 +0.7 748,304 + RR42_RS24170 0.13 -0.1 748,304 + RR42_RS24170 0.13 -0.3 748,359 + RR42_RS24170 0.20 +0.0 748,449 - RR42_RS24170 0.30 +1.0 748,492 - RR42_RS24170 0.35 -0.5 748,754 - RR42_RS24170 0.66 -0.5 748,816 - RR42_RS24170 0.73 +0.6 748,816 - RR42_RS24170 0.73 +0.9 748,816 - RR42_RS24170 0.73 +0.1 748,877 + RR42_RS24170 0.80 +0.9 748,877 + RR42_RS24170 0.80 +2.5 748,878 - RR42_RS24170 0.80 -2.0 748,878 - RR42_RS24170 0.80 +0.4 748,878 - RR42_RS24170 0.80 -1.2 748,916 + RR42_RS24170 0.85 -1.3 748,916 + RR42_RS24170 0.85 -3.0 748,916 + RR42_RS24170 0.85 -1.3 748,938 + RR42_RS24170 0.87 +0.0 748,939 - RR42_RS24170 0.87 +0.4 748,979 + +1.6 748,979 + +0.5 749,045 + -0.3 749,046 - +0.3 749,303 - -1.8 749,362 + -0.9 749,362 + +0.8 749,362 + -1.7 749,363 - -0.1 749,363 - -0.1 749,677 - RR42_RS24175 0.28 -1.1 750,010 + RR42_RS24175 0.51 -1.5 750,044 + RR42_RS24175 0.54 +2.1 750,045 - RR42_RS24175 0.54 +0.3 750,259 + RR42_RS24175 0.69 -1.1 750,259 + RR42_RS24175 0.69 +0.2 750,259 + RR42_RS24175 0.69 -2.6 750,260 - RR42_RS24175 0.69 -0.5 750,260 - RR42_RS24175 0.69 -0.5 750,260 - RR42_RS24175 0.69 -0.4 750,340 - RR42_RS24175 0.75 +0.0 750,340 - RR42_RS24175 0.75 -0.4 750,586 - -0.1 750,586 - +0.8 750,659 + +0.3 750,659 + -0.1 750,660 - -0.1 750,660 - +1.0 750,702 + -0.1 750,703 - -0.8 750,706 + -1.8 750,706 + +1.7 750,707 - -3.9 750,742 + -0.5 750,742 + +0.6 750,742 + +0.2 750,742 + +0.2 750,931 + RR42_RS24180 0.31 +1.3 751,087 - RR42_RS24180 0.55 +1.3 751,167 + RR42_RS24180 0.68 -0.6 751,167 + RR42_RS24180 0.68 +0.7 751,168 - RR42_RS24180 0.68 -0.6 751,225 - RR42_RS24180 0.77 +2.0 751,254 + RR42_RS24180 0.81 +1.3 751,255 - RR42_RS24180 0.81 -1.6 751,379 + +0.0 751,379 + -0.8 751,379 + +0.2 751,379 + -0.8 751,379 + +0.3 751,380 - +2.2 751,457 + -2.3 751,458 - -0.7 751,472 + +0.2 751,499 + +0.6 751,521 - -0.8 751,557 + +0.3 751,558 - -0.4 751,601 + +1.2 751,617 + -1.1 751,618 - +0.3 751,630 + -1.1 751,630 + +0.3 751,669 + -0.8 751,669 + +0.3 751,692 + -0.1 751,692 + +1.2
Or see this region's nucleotide sequence