Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23575 and RR42_RS23580 are separated by 516 nucleotides RR42_RS23580 and RR42_RS23585 are separated by 161 nucleotides RR42_RS23585 and RR42_RS23590 are separated by 8 nucleotides
RR42_RS23575: RR42_RS23575 - flagellar hook-basal body protein FliE, at 615,571 to 615,891
_RS23575
RR42_RS23580: RR42_RS23580 - hypothetical protein, at 616,408 to 617,061
_RS23580
RR42_RS23585: RR42_RS23585 - hypothetical protein, at 617,223 to 617,873
_RS23585
RR42_RS23590: RR42_RS23590 - FkbM family methyltransferase, at 617,882 to 618,952
_RS23590
Position (kb) 616
617
618 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 615.421 kb on + strand at 615.492 kb on + strand at 615.492 kb on + strand at 615.493 kb on - strand at 615.576 kb on + strand at 615.576 kb on + strand at 615.576 kb on + strand at 615.577 kb on - strand at 615.577 kb on - strand at 615.810 kb on + strand, within RR42_RS23575 at 615.811 kb on - strand, within RR42_RS23575 at 615.811 kb on - strand, within RR42_RS23575 at 615.879 kb on + strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 616.255 kb on + strand at 616.255 kb on + strand at 616.256 kb on - strand at 616.410 kb on - strand at 616.410 kb on - strand at 616.422 kb on - strand at 616.482 kb on - strand, within RR42_RS23580 at 616.586 kb on + strand, within RR42_RS23580 at 616.636 kb on + strand, within RR42_RS23580 at 616.664 kb on + strand, within RR42_RS23580 at 616.664 kb on + strand, within RR42_RS23580 at 616.665 kb on - strand, within RR42_RS23580 at 616.674 kb on - strand, within RR42_RS23580 at 616.742 kb on + strand, within RR42_RS23580 at 616.742 kb on + strand, within RR42_RS23580 at 616.838 kb on + strand, within RR42_RS23580 at 616.838 kb on + strand, within RR42_RS23580 at 616.838 kb on + strand, within RR42_RS23580 at 616.839 kb on - strand, within RR42_RS23580 at 616.839 kb on - strand, within RR42_RS23580 at 616.841 kb on + strand, within RR42_RS23580 at 616.841 kb on + strand, within RR42_RS23580 at 616.841 kb on + strand, within RR42_RS23580 at 616.847 kb on + strand, within RR42_RS23580 at 616.847 kb on + strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.856 kb on + strand, within RR42_RS23580 at 616.903 kb on + strand, within RR42_RS23580 at 616.903 kb on + strand, within RR42_RS23580 at 617.022 kb on - strand at 617.116 kb on - strand at 617.326 kb on + strand, within RR42_RS23585 at 617.326 kb on + strand, within RR42_RS23585 at 617.327 kb on - strand, within RR42_RS23585 at 617.327 kb on - strand, within RR42_RS23585 at 617.327 kb on - strand, within RR42_RS23585 at 617.515 kb on + strand, within RR42_RS23585 at 617.590 kb on + strand, within RR42_RS23585 at 617.591 kb on - strand at 617.722 kb on + strand, within RR42_RS23585 at 617.722 kb on + strand, within RR42_RS23585 at 617.723 kb on - strand, within RR42_RS23585 at 617.723 kb on - strand, within RR42_RS23585 at 617.853 kb on - strand at 618.027 kb on + strand, within RR42_RS23590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 615,421 + -0.6 615,492 + +0.7 615,492 + -0.9 615,493 - +0.2 615,576 + -0.1 615,576 + +0.2 615,576 + -2.4 615,577 - +0.7 615,577 - -0.8 615,810 + RR42_RS23575 0.74 +0.1 615,811 - RR42_RS23575 0.75 -0.5 615,811 - RR42_RS23575 0.75 +0.4 615,879 + +1.5 615,880 - -2.0 615,880 - -0.9 615,880 - -0.8 615,880 - -0.1 615,880 - -0.2 616,255 + -2.6 616,255 + +0.3 616,256 - -0.2 616,410 - -0.6 616,410 - -2.0 616,422 - -1.5 616,482 - RR42_RS23580 0.11 +0.9 616,586 + RR42_RS23580 0.27 +1.3 616,636 + RR42_RS23580 0.35 -3.6 616,664 + RR42_RS23580 0.39 +1.1 616,664 + RR42_RS23580 0.39 -0.7 616,665 - RR42_RS23580 0.39 -0.7 616,674 - RR42_RS23580 0.41 -0.5 616,742 + RR42_RS23580 0.51 +0.7 616,742 + RR42_RS23580 0.51 -1.3 616,838 + RR42_RS23580 0.66 -2.0 616,838 + RR42_RS23580 0.66 -1.2 616,838 + RR42_RS23580 0.66 -0.4 616,839 - RR42_RS23580 0.66 +1.3 616,839 - RR42_RS23580 0.66 -0.5 616,841 + RR42_RS23580 0.66 -0.0 616,841 + RR42_RS23580 0.66 +1.4 616,841 + RR42_RS23580 0.66 -1.8 616,847 + RR42_RS23580 0.67 -1.0 616,847 + RR42_RS23580 0.67 +1.1 616,848 - RR42_RS23580 0.67 -2.2 616,848 - RR42_RS23580 0.67 -0.0 616,848 - RR42_RS23580 0.67 -0.3 616,848 - RR42_RS23580 0.67 -0.3 616,856 + RR42_RS23580 0.69 -0.9 616,903 + RR42_RS23580 0.76 +0.0 616,903 + RR42_RS23580 0.76 -1.4 617,022 - -0.7 617,116 - +0.7 617,326 + RR42_RS23585 0.16 -0.1 617,326 + RR42_RS23585 0.16 -1.0 617,327 - RR42_RS23585 0.16 +0.7 617,327 - RR42_RS23585 0.16 -0.3 617,327 - RR42_RS23585 0.16 +0.9 617,515 + RR42_RS23585 0.45 +1.7 617,590 + RR42_RS23585 0.56 -1.3 617,591 - +1.1 617,722 + RR42_RS23585 0.77 +0.9 617,722 + RR42_RS23585 0.77 -1.5 617,723 - RR42_RS23585 0.77 +0.2 617,723 - RR42_RS23585 0.77 -1.0 617,853 - -1.9 618,027 + RR42_RS23590 0.14 -0.2
Or see this region's nucleotide sequence