Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23535 and RR42_RS23540 are separated by 30 nucleotides RR42_RS23540 and RR42_RS23545 are separated by 134 nucleotides RR42_RS23545 and RR42_RS23550 are separated by 26 nucleotides RR42_RS23550 and RR42_RS23555 overlap by 8 nucleotides
RR42_RS23535: RR42_RS23535 - hypothetical protein, at 606,107 to 607,333
_RS23535
RR42_RS23540: RR42_RS23540 - alpha-ketoglutarate-dependent dioxygenase, at 607,364 to 608,017
_RS23540
RR42_RS23545: RR42_RS23545 - hypothetical protein, at 608,152 to 609,594
_RS23545
RR42_RS23550: RR42_RS23550 - flagellar export protein FliJ, at 609,621 to 610,076
_RS23550
RR42_RS23555: RR42_RS23555 - ATP synthase, at 610,069 to 611,532
_RS23555
Position (kb) 608
609
610 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 607.165 kb on - strand, within RR42_RS23535 at 607.274 kb on + strand at 607.365 kb on + strand at 607.476 kb on - strand, within RR42_RS23540 at 607.900 kb on + strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 608.081 kb on + strand at 608.081 kb on + strand at 608.082 kb on - strand at 608.082 kb on - strand at 608.082 kb on - strand at 608.106 kb on - strand at 608.819 kb on + strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 609.075 kb on + strand, within RR42_RS23545 at 609.148 kb on - strand, within RR42_RS23545 at 609.334 kb on + strand, within RR42_RS23545 at 609.335 kb on - strand, within RR42_RS23545 at 609.585 kb on + strand at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.668 kb on - strand, within RR42_RS23550 at 609.732 kb on - strand, within RR42_RS23550 at 609.732 kb on - strand, within RR42_RS23550 at 610.052 kb on + strand at 610.053 kb on - strand at 610.070 kb on + strand at 610.070 kb on + strand at 610.070 kb on + strand at 610.071 kb on - strand at 610.098 kb on + strand at 610.098 kb on + strand at 610.098 kb on + strand at 610.099 kb on - strand at 610.099 kb on - strand at 610.220 kb on + strand, within RR42_RS23555 at 610.221 kb on - strand, within RR42_RS23555 at 610.257 kb on - strand, within RR42_RS23555 at 610.266 kb on + strand, within RR42_RS23555 at 610.472 kb on + strand, within RR42_RS23555 at 610.472 kb on + strand, within RR42_RS23555 at 610.473 kb on - strand, within RR42_RS23555
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 607,165 - RR42_RS23535 0.86 +1.1 607,274 + +1.3 607,365 + +1.5 607,476 - RR42_RS23540 0.17 -2.9 607,900 + RR42_RS23540 0.82 -0.5 607,901 - RR42_RS23540 0.82 -0.0 607,901 - RR42_RS23540 0.82 +2.3 607,901 - RR42_RS23540 0.82 +0.5 608,081 + -1.0 608,081 + +1.6 608,082 - -1.5 608,082 - +0.1 608,082 - -2.1 608,106 - +0.0 608,819 + RR42_RS23545 0.46 -1.2 608,820 - RR42_RS23545 0.46 -1.7 608,820 - RR42_RS23545 0.46 -2.5 608,820 - RR42_RS23545 0.46 -0.5 609,075 + RR42_RS23545 0.64 +0.5 609,148 - RR42_RS23545 0.69 +0.1 609,334 + RR42_RS23545 0.82 -3.1 609,335 - RR42_RS23545 0.82 -0.7 609,585 + +0.4 609,667 + RR42_RS23550 0.10 +0.4 609,667 + RR42_RS23550 0.10 -0.9 609,667 + RR42_RS23550 0.10 -0.0 609,667 + RR42_RS23550 0.10 -0.9 609,668 - RR42_RS23550 0.10 -1.1 609,732 - RR42_RS23550 0.24 +0.9 609,732 - RR42_RS23550 0.24 -1.0 610,052 + -1.7 610,053 - +0.1 610,070 + +0.7 610,070 + -1.5 610,070 + +0.2 610,071 - +0.1 610,098 + +0.6 610,098 + +1.6 610,098 + -0.7 610,099 - +0.9 610,099 - -1.2 610,220 + RR42_RS23555 0.10 -1.4 610,221 - RR42_RS23555 0.10 -0.9 610,257 - RR42_RS23555 0.13 +1.5 610,266 + RR42_RS23555 0.13 -1.4 610,472 + RR42_RS23555 0.28 -0.9 610,472 + RR42_RS23555 0.28 +0.8 610,473 - RR42_RS23555 0.28 -1.3
Or see this region's nucleotide sequence