Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23535 and RR42_RS23540 are separated by 30 nucleotides RR42_RS23540 and RR42_RS23545 are separated by 134 nucleotides
RR42_RS23535: RR42_RS23535 - hypothetical protein, at 606,107 to 607,333
_RS23535
RR42_RS23540: RR42_RS23540 - alpha-ketoglutarate-dependent dioxygenase, at 607,364 to 608,017
_RS23540
RR42_RS23545: RR42_RS23545 - hypothetical protein, at 608,152 to 609,594
_RS23545
Position (kb) 607
608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 606.647 kb on + strand, within RR42_RS23535 at 606.648 kb on - strand, within RR42_RS23535 at 606.648 kb on - strand, within RR42_RS23535 at 606.648 kb on - strand, within RR42_RS23535 at 606.671 kb on + strand, within RR42_RS23535 at 606.671 kb on + strand, within RR42_RS23535 at 606.720 kb on + strand, within RR42_RS23535 at 606.721 kb on - strand, within RR42_RS23535 at 606.866 kb on + strand, within RR42_RS23535 at 606.867 kb on - strand, within RR42_RS23535 at 606.989 kb on + strand, within RR42_RS23535 at 606.989 kb on + strand, within RR42_RS23535 at 606.989 kb on + strand, within RR42_RS23535 at 606.989 kb on + strand, within RR42_RS23535 at 606.990 kb on - strand, within RR42_RS23535 at 606.990 kb on - strand, within RR42_RS23535 at 607.058 kb on + strand, within RR42_RS23535 at 607.058 kb on + strand, within RR42_RS23535 at 607.059 kb on - strand, within RR42_RS23535 at 607.059 kb on - strand, within RR42_RS23535 at 607.059 kb on - strand, within RR42_RS23535 at 607.059 kb on - strand, within RR42_RS23535 at 607.067 kb on + strand, within RR42_RS23535 at 607.165 kb on - strand, within RR42_RS23535 at 607.274 kb on + strand at 607.365 kb on + strand at 607.476 kb on - strand, within RR42_RS23540 at 607.900 kb on + strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 608.081 kb on + strand at 608.081 kb on + strand at 608.082 kb on - strand at 608.082 kb on - strand at 608.082 kb on - strand at 608.106 kb on - strand at 608.819 kb on + strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 606,647 + RR42_RS23535 0.44 +0.6 606,648 - RR42_RS23535 0.44 +2.0 606,648 - RR42_RS23535 0.44 +0.4 606,648 - RR42_RS23535 0.44 -3.6 606,671 + RR42_RS23535 0.46 -0.1 606,671 + RR42_RS23535 0.46 +0.9 606,720 + RR42_RS23535 0.50 -0.3 606,721 - RR42_RS23535 0.50 -0.4 606,866 + RR42_RS23535 0.62 -3.1 606,867 - RR42_RS23535 0.62 -0.2 606,989 + RR42_RS23535 0.72 +0.8 606,989 + RR42_RS23535 0.72 -1.2 606,989 + RR42_RS23535 0.72 -0.6 606,989 + RR42_RS23535 0.72 +2.0 606,990 - RR42_RS23535 0.72 +0.3 606,990 - RR42_RS23535 0.72 +0.3 607,058 + RR42_RS23535 0.78 +1.0 607,058 + RR42_RS23535 0.78 -1.0 607,059 - RR42_RS23535 0.78 -1.2 607,059 - RR42_RS23535 0.78 +1.0 607,059 - RR42_RS23535 0.78 +0.7 607,059 - RR42_RS23535 0.78 +0.4 607,067 + RR42_RS23535 0.78 -0.1 607,165 - RR42_RS23535 0.86 +1.1 607,274 + +1.3 607,365 + +1.5 607,476 - RR42_RS23540 0.17 -2.9 607,900 + RR42_RS23540 0.82 -0.5 607,901 - RR42_RS23540 0.82 -0.0 607,901 - RR42_RS23540 0.82 +2.3 607,901 - RR42_RS23540 0.82 +0.5 608,081 + -1.0 608,081 + +1.6 608,082 - -1.5 608,082 - +0.1 608,082 - -2.1 608,106 - +0.0 608,819 + RR42_RS23545 0.46 -1.2 608,820 - RR42_RS23545 0.46 -1.7 608,820 - RR42_RS23545 0.46 -2.5 608,820 - RR42_RS23545 0.46 -0.5
Or see this region's nucleotide sequence