Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23490 and RR42_RS23495 are separated by 25 nucleotides RR42_RS23495 and RR42_RS23500 are separated by 59 nucleotides RR42_RS23500 and RR42_RS23505 are separated by 132 nucleotides
RR42_RS23490: RR42_RS23490 - NADH oxidase, at 596,157 to 597,176
_RS23490
RR42_RS23495: RR42_RS23495 - benzene 1,2-dioxygenase, at 597,202 to 597,684
_RS23495
RR42_RS23500: RR42_RS23500 - benzene 1,2-dioxygenase, at 597,744 to 599,132
_RS23500
RR42_RS23505: RR42_RS23505 - catechol 1,2-dioxygenase, at 599,265 to 600,179
_RS23505
Position (kb)
597
598
599
600 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.753 kb on - strand, within RR42_RS23490 at 596.984 kb on + strand, within RR42_RS23490 at 596.985 kb on - strand, within RR42_RS23490 at 596.986 kb on + strand, within RR42_RS23490 at 596.987 kb on - strand, within RR42_RS23490 at 597.169 kb on + strand at 597.169 kb on + strand at 597.169 kb on + strand at 597.169 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.213 kb on - strand at 597.213 kb on - strand at 597.213 kb on - strand at 597.236 kb on + strand at 597.257 kb on + strand, within RR42_RS23495 at 597.303 kb on - strand, within RR42_RS23495 at 597.326 kb on + strand, within RR42_RS23495 at 597.327 kb on - strand, within RR42_RS23495 at 597.327 kb on - strand, within RR42_RS23495 at 597.599 kb on + strand, within RR42_RS23495 at 597.818 kb on - strand at 598.045 kb on + strand, within RR42_RS23500 at 598.102 kb on + strand, within RR42_RS23500 at 598.102 kb on + strand, within RR42_RS23500 at 598.339 kb on + strand, within RR42_RS23500 at 598.425 kb on - strand, within RR42_RS23500 at 598.425 kb on - strand, within RR42_RS23500 at 598.438 kb on + strand, within RR42_RS23500 at 598.514 kb on + strand, within RR42_RS23500 at 598.516 kb on + strand, within RR42_RS23500 at 598.516 kb on + strand, within RR42_RS23500 at 598.517 kb on - strand, within RR42_RS23500 at 598.517 kb on - strand, within RR42_RS23500 at 598.795 kb on + strand, within RR42_RS23500 at 598.796 kb on - strand, within RR42_RS23500 at 598.796 kb on - strand, within RR42_RS23500 at 598.918 kb on + strand, within RR42_RS23500 at 598.919 kb on - strand, within RR42_RS23500 at 598.927 kb on + strand, within RR42_RS23500 at 598.927 kb on + strand, within RR42_RS23500 at 598.928 kb on - strand, within RR42_RS23500 at 598.931 kb on - strand, within RR42_RS23500 at 598.931 kb on - strand, within RR42_RS23500 at 598.969 kb on + strand, within RR42_RS23500 at 598.969 kb on + strand, within RR42_RS23500 at 599.120 kb on - strand at 599.120 kb on - strand at 599.267 kb on - strand at 599.603 kb on - strand, within RR42_RS23505 at 599.603 kb on - strand, within RR42_RS23505 at 599.704 kb on + strand, within RR42_RS23505 at 599.704 kb on + strand, within RR42_RS23505 at 599.704 kb on + strand, within RR42_RS23505 at 599.705 kb on - strand, within RR42_RS23505 at 599.869 kb on + strand, within RR42_RS23505 at 599.869 kb on + strand, within RR42_RS23505 at 599.869 kb on + strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 599.870 kb on - strand, within RR42_RS23505 at 600.010 kb on + strand, within RR42_RS23505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 596,752 + RR42_RS23490 0.58 -0.0 596,752 + RR42_RS23490 0.58 +1.2 596,752 + RR42_RS23490 0.58 +0.8 596,752 + RR42_RS23490 0.58 +1.2 596,752 + RR42_RS23490 0.58 -0.9 596,753 - RR42_RS23490 0.58 +2.1 596,984 + RR42_RS23490 0.81 +1.3 596,985 - RR42_RS23490 0.81 +0.1 596,986 + RR42_RS23490 0.81 -0.2 596,987 - RR42_RS23490 0.81 +0.5 597,169 + +0.7 597,169 + -0.4 597,169 + +1.1 597,169 + -2.1 597,212 + +0.6 597,212 + +0.6 597,212 + +0.5 597,212 + -0.5 597,213 - +0.8 597,213 - -1.3 597,213 - +0.6 597,236 + -0.3 597,257 + RR42_RS23495 0.11 -0.0 597,303 - RR42_RS23495 0.21 +0.9 597,326 + RR42_RS23495 0.26 -0.1 597,327 - RR42_RS23495 0.26 +0.6 597,327 - RR42_RS23495 0.26 +0.5 597,599 + RR42_RS23495 0.82 +0.7 597,818 - -1.2 598,045 + RR42_RS23500 0.22 -0.3 598,102 + RR42_RS23500 0.26 -0.5 598,102 + RR42_RS23500 0.26 +0.1 598,339 + RR42_RS23500 0.43 -0.5 598,425 - RR42_RS23500 0.49 +1.1 598,425 - RR42_RS23500 0.49 -3.1 598,438 + RR42_RS23500 0.50 -1.7 598,514 + RR42_RS23500 0.55 +0.1 598,516 + RR42_RS23500 0.56 -1.6 598,516 + RR42_RS23500 0.56 -2.1 598,517 - RR42_RS23500 0.56 +0.4 598,517 - RR42_RS23500 0.56 +1.0 598,795 + RR42_RS23500 0.76 -1.8 598,796 - RR42_RS23500 0.76 -0.3 598,796 - RR42_RS23500 0.76 +0.6 598,918 + RR42_RS23500 0.85 -2.2 598,919 - RR42_RS23500 0.85 -0.2 598,927 + RR42_RS23500 0.85 +0.7 598,927 + RR42_RS23500 0.85 -1.7 598,928 - RR42_RS23500 0.85 -2.1 598,931 - RR42_RS23500 0.85 +1.1 598,931 - RR42_RS23500 0.85 +0.2 598,969 + RR42_RS23500 0.88 +1.1 598,969 + RR42_RS23500 0.88 -1.5 599,120 - -0.5 599,120 - +0.7 599,267 - +1.3 599,603 - RR42_RS23505 0.37 +0.9 599,603 - RR42_RS23505 0.37 -2.1 599,704 + RR42_RS23505 0.48 +0.3 599,704 + RR42_RS23505 0.48 -1.0 599,704 + RR42_RS23505 0.48 -2.9 599,705 - RR42_RS23505 0.48 -0.4 599,869 + RR42_RS23505 0.66 +0.2 599,869 + RR42_RS23505 0.66 +0.3 599,869 + RR42_RS23505 0.66 -2.1 599,870 - RR42_RS23505 0.66 +1.4 599,870 - RR42_RS23505 0.66 -0.6 599,870 - RR42_RS23505 0.66 -1.9 599,870 - RR42_RS23505 0.66 -0.8 599,870 - RR42_RS23505 0.66 +0.9 599,870 - RR42_RS23505 0.66 -0.0 599,870 - RR42_RS23505 0.66 +0.7 599,870 - RR42_RS23505 0.66 +0.5 600,010 + RR42_RS23505 0.81 +0.4
Or see this region's nucleotide sequence