Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23475 and RR42_RS23480 are separated by 324 nucleotides RR42_RS23480 and RR42_RS23485 are separated by 152 nucleotides RR42_RS23485 and RR42_RS23490 are separated by 28 nucleotides
RR42_RS23475: RR42_RS23475 - IclR family transcriptional regulator, at 593,229 to 594,032
_RS23475
RR42_RS23480: RR42_RS23480 - 3-keto-5-aminohexanoate cleavage protein, at 594,357 to 595,190
_RS23480
RR42_RS23485: RR42_RS23485 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 595,343 to 596,128
_RS23485
RR42_RS23490: RR42_RS23490 - NADH oxidase, at 596,157 to 597,176
_RS23490
Position (kb) 594
595
596 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 593.358 kb on - strand, within RR42_RS23475 at 593.372 kb on + strand, within RR42_RS23475 at 593.442 kb on + strand, within RR42_RS23475 at 593.443 kb on - strand, within RR42_RS23475 at 593.614 kb on - strand, within RR42_RS23475 at 593.655 kb on - strand, within RR42_RS23475 at 593.657 kb on + strand, within RR42_RS23475 at 593.658 kb on - strand, within RR42_RS23475 at 593.675 kb on + strand, within RR42_RS23475 at 593.676 kb on - strand, within RR42_RS23475 at 593.710 kb on - strand, within RR42_RS23475 at 593.841 kb on + strand, within RR42_RS23475 at 593.841 kb on + strand, within RR42_RS23475 at 593.913 kb on + strand, within RR42_RS23475 at 593.913 kb on + strand, within RR42_RS23475 at 593.913 kb on + strand, within RR42_RS23475 at 593.913 kb on + strand, within RR42_RS23475 at 593.914 kb on - strand, within RR42_RS23475 at 593.914 kb on - strand, within RR42_RS23475 at 594.121 kb on + strand at 594.122 kb on - strand at 594.122 kb on - strand at 594.245 kb on + strand at 594.246 kb on - strand at 594.249 kb on + strand at 594.249 kb on + strand at 594.249 kb on + strand at 594.249 kb on + strand at 594.330 kb on + strand at 594.330 kb on + strand at 594.331 kb on - strand at 594.331 kb on - strand at 594.342 kb on + strand at 594.342 kb on + strand at 594.343 kb on - strand at 594.343 kb on - strand at 594.350 kb on + strand at 594.350 kb on + strand at 594.480 kb on - strand, within RR42_RS23480 at 594.480 kb on - strand, within RR42_RS23480 at 594.628 kb on + strand, within RR42_RS23480 at 594.750 kb on + strand, within RR42_RS23480 at 594.751 kb on - strand, within RR42_RS23480 at 594.751 kb on - strand, within RR42_RS23480 at 594.751 kb on - strand, within RR42_RS23480 at 594.785 kb on - strand, within RR42_RS23480 at 594.954 kb on - strand, within RR42_RS23480 at 594.991 kb on - strand, within RR42_RS23480 at 595.113 kb on - strand at 595.113 kb on - strand at 595.173 kb on - strand at 595.195 kb on - strand at 595.214 kb on + strand at 595.214 kb on + strand at 595.215 kb on - strand at 595.217 kb on - strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.390 kb on - strand at 595.390 kb on - strand at 595.497 kb on + strand, within RR42_RS23485 at 595.674 kb on + strand, within RR42_RS23485 at 595.675 kb on - strand, within RR42_RS23485 at 595.675 kb on - strand, within RR42_RS23485 at 596.128 kb on + strand at 596.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 593,358 - RR42_RS23475 0.16 -0.5 593,372 + RR42_RS23475 0.18 +0.2 593,442 + RR42_RS23475 0.26 -0.7 593,443 - RR42_RS23475 0.27 -0.5 593,614 - RR42_RS23475 0.48 +1.3 593,655 - RR42_RS23475 0.53 +0.5 593,657 + RR42_RS23475 0.53 -0.3 593,658 - RR42_RS23475 0.53 -0.3 593,675 + RR42_RS23475 0.55 +1.1 593,676 - RR42_RS23475 0.56 +0.4 593,710 - RR42_RS23475 0.60 -0.3 593,841 + RR42_RS23475 0.76 +0.4 593,841 + RR42_RS23475 0.76 +0.5 593,913 + RR42_RS23475 0.85 +0.8 593,913 + RR42_RS23475 0.85 +0.1 593,913 + RR42_RS23475 0.85 -0.0 593,913 + RR42_RS23475 0.85 -2.3 593,914 - RR42_RS23475 0.85 +0.5 593,914 - RR42_RS23475 0.85 -0.6 594,121 + +0.8 594,122 - +0.1 594,122 - -1.9 594,245 + -0.2 594,246 - +2.8 594,249 + +1.6 594,249 + +0.1 594,249 + +0.1 594,249 + -0.4 594,330 + +0.3 594,330 + -0.0 594,331 - -0.8 594,331 - -0.2 594,342 + +1.3 594,342 + +3.4 594,343 - -0.2 594,343 - -0.2 594,350 + +0.2 594,350 + -0.3 594,480 - RR42_RS23480 0.15 -4.2 594,480 - RR42_RS23480 0.15 +0.8 594,628 + RR42_RS23480 0.32 -1.2 594,750 + RR42_RS23480 0.47 -2.2 594,751 - RR42_RS23480 0.47 +0.1 594,751 - RR42_RS23480 0.47 -2.3 594,751 - RR42_RS23480 0.47 -1.1 594,785 - RR42_RS23480 0.51 -0.6 594,954 - RR42_RS23480 0.72 +0.4 594,991 - RR42_RS23480 0.76 -2.2 595,113 - -0.1 595,113 - +1.3 595,173 - +1.0 595,195 - +1.8 595,214 + -1.2 595,214 + +1.7 595,215 - -2.7 595,217 - +1.4 595,389 + -0.2 595,389 + +0.4 595,389 + -1.5 595,389 + +0.4 595,389 + +1.3 595,389 + +0.6 595,390 - -0.8 595,390 - +0.1 595,497 + RR42_RS23485 0.20 -0.4 595,674 + RR42_RS23485 0.42 -0.9 595,675 - RR42_RS23485 0.42 +0.1 595,675 - RR42_RS23485 0.42 +0.5 596,128 + -0.5 596,129 - -1.0
Or see this region's nucleotide sequence