Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23410 and RR42_RS23415 are separated by 225 nucleotides RR42_RS23415 and RR42_RS23420 are separated by 54 nucleotides RR42_RS23420 and RR42_RS23425 are separated by 44 nucleotides
RR42_RS23410: RR42_RS23410 - hypothetical protein, at 579,749 to 580,648
_RS23410
RR42_RS23415: RR42_RS23415 - 4-carboxymuconolactone decarboxylase, at 580,874 to 581,428
_RS23415
RR42_RS23420: RR42_RS23420 - 3-oxoacyl-ACP reductase, at 581,483 to 582,283
_RS23420
RR42_RS23425: RR42_RS23425 - hypothetical protein, at 582,328 to 583,323
_RS23425
Position (kb) 580
581
582 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 579.947 kb on + strand, within RR42_RS23410 at 579.981 kb on + strand, within RR42_RS23410 at 580.041 kb on + strand, within RR42_RS23410 at 580.041 kb on + strand, within RR42_RS23410 at 580.042 kb on - strand, within RR42_RS23410 at 580.202 kb on + strand, within RR42_RS23410 at 580.202 kb on + strand, within RR42_RS23410 at 580.203 kb on - strand, within RR42_RS23410 at 580.338 kb on + strand, within RR42_RS23410 at 580.338 kb on + strand, within RR42_RS23410 at 580.486 kb on + strand, within RR42_RS23410 at 580.697 kb on - strand at 580.727 kb on + strand at 580.728 kb on - strand at 580.728 kb on - strand at 580.762 kb on + strand at 580.965 kb on + strand, within RR42_RS23415 at 580.965 kb on + strand, within RR42_RS23415 at 580.965 kb on + strand, within RR42_RS23415 at 581.033 kb on + strand, within RR42_RS23415 at 581.034 kb on - strand, within RR42_RS23415 at 581.034 kb on - strand, within RR42_RS23415 at 581.243 kb on - strand, within RR42_RS23415 at 581.282 kb on + strand, within RR42_RS23415 at 581.282 kb on + strand, within RR42_RS23415 at 581.283 kb on - strand, within RR42_RS23415 at 581.322 kb on + strand, within RR42_RS23415 at 581.348 kb on + strand, within RR42_RS23415 at 581.476 kb on - strand at 581.476 kb on - strand at 581.834 kb on - strand, within RR42_RS23420 at 581.966 kb on + strand, within RR42_RS23420 at 582.020 kb on + strand, within RR42_RS23420 at 582.399 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 579,947 + RR42_RS23410 0.22 -2.9 579,981 + RR42_RS23410 0.26 -1.2 580,041 + RR42_RS23410 0.32 +0.8 580,041 + RR42_RS23410 0.32 -0.9 580,042 - RR42_RS23410 0.33 -0.8 580,202 + RR42_RS23410 0.50 -0.9 580,202 + RR42_RS23410 0.50 -1.4 580,203 - RR42_RS23410 0.50 -0.4 580,338 + RR42_RS23410 0.65 +1.2 580,338 + RR42_RS23410 0.65 +0.6 580,486 + RR42_RS23410 0.82 -2.7 580,697 - +1.3 580,727 + -1.4 580,728 - -0.4 580,728 - -0.0 580,762 + +0.3 580,965 + RR42_RS23415 0.16 -1.2 580,965 + RR42_RS23415 0.16 -0.7 580,965 + RR42_RS23415 0.16 +0.1 581,033 + RR42_RS23415 0.29 -1.2 581,034 - RR42_RS23415 0.29 +0.3 581,034 - RR42_RS23415 0.29 +1.8 581,243 - RR42_RS23415 0.66 -1.5 581,282 + RR42_RS23415 0.74 -2.2 581,282 + RR42_RS23415 0.74 -0.4 581,283 - RR42_RS23415 0.74 +1.2 581,322 + RR42_RS23415 0.81 +0.3 581,348 + RR42_RS23415 0.85 -0.8 581,476 - +0.2 581,476 - -1.1 581,834 - RR42_RS23420 0.44 -0.1 581,966 + RR42_RS23420 0.60 -1.3 582,020 + RR42_RS23420 0.67 -0.3 582,399 - -1.8
Or see this region's nucleotide sequence