Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23330 and RR42_RS23335 overlap by 9 nucleotides RR42_RS23335 and RR42_RS23340 are separated by 491 nucleotides RR42_RS23340 and RR42_RS23345 are separated by 104 nucleotides
RR42_RS23330: RR42_RS23330 - class A beta-lactamase, at 565,293 to 566,180
_RS23330
RR42_RS23335: RR42_RS23335 - hypothetical protein, at 566,172 to 566,552
_RS23335
RR42_RS23340: RR42_RS23340 - RNA ligase, at 567,044 to 568,270
_RS23340
RR42_RS23345: RR42_RS23345 - hypothetical protein, at 568,375 to 569,220
_RS23345
Position (kb) 567
568
569 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 566.174 kb on - strand at 566.178 kb on + strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.224 kb on - strand, within RR42_RS23335 at 566.274 kb on - strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.348 kb on - strand, within RR42_RS23335 at 566.348 kb on - strand, within RR42_RS23335 at 566.849 kb on + strand at 566.850 kb on - strand at 566.850 kb on - strand at 566.919 kb on - strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.945 kb on + strand at 566.945 kb on + strand at 566.945 kb on + strand at 566.946 kb on - strand at 566.981 kb on + strand at 566.982 kb on - strand at 566.982 kb on - strand at 566.982 kb on - strand at 566.982 kb on - strand at 567.011 kb on + strand at 567.060 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.202 kb on - strand, within RR42_RS23340 at 567.202 kb on - strand, within RR42_RS23340 at 567.497 kb on + strand, within RR42_RS23340 at 567.498 kb on - strand, within RR42_RS23340 at 567.652 kb on + strand, within RR42_RS23340 at 567.750 kb on - strand, within RR42_RS23340 at 567.886 kb on + strand, within RR42_RS23340 at 567.886 kb on + strand, within RR42_RS23340 at 567.887 kb on - strand, within RR42_RS23340 at 567.887 kb on - strand, within RR42_RS23340 at 567.887 kb on - strand, within RR42_RS23340 at 567.890 kb on + strand, within RR42_RS23340 at 567.890 kb on + strand, within RR42_RS23340 at 567.890 kb on + strand, within RR42_RS23340 at 568.005 kb on - strand, within RR42_RS23340 at 568.005 kb on - strand, within RR42_RS23340 at 568.182 kb on - strand at 568.486 kb on + strand, within RR42_RS23345 at 568.667 kb on + strand, within RR42_RS23345 at 568.667 kb on + strand, within RR42_RS23345 at 568.667 kb on + strand, within RR42_RS23345 at 568.667 kb on + strand, within RR42_RS23345 at 568.668 kb on - strand, within RR42_RS23345 at 568.668 kb on - strand, within RR42_RS23345 at 568.668 kb on - strand, within RR42_RS23345 at 568.862 kb on - strand, within RR42_RS23345 at 569.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 566,174 - -0.6 566,178 + +0.6 566,179 - -0.9 566,179 - +1.3 566,179 - +0.2 566,179 - +0.2 566,179 - +0.3 566,224 - RR42_RS23335 0.14 -0.0 566,274 - RR42_RS23335 0.27 -2.3 566,347 + RR42_RS23335 0.46 +0.1 566,347 + RR42_RS23335 0.46 -1.9 566,347 + RR42_RS23335 0.46 +0.1 566,347 + RR42_RS23335 0.46 +0.6 566,348 - RR42_RS23335 0.46 +0.1 566,348 - RR42_RS23335 0.46 -2.8 566,849 + +0.0 566,850 - +0.5 566,850 - +2.1 566,919 - +1.0 566,921 + -0.3 566,921 + +1.1 566,921 + +0.9 566,921 + -0.9 566,921 + -0.7 566,922 - +1.0 566,922 - -0.0 566,922 - -0.0 566,922 - +1.4 566,945 + -0.4 566,945 + -0.6 566,945 + +0.6 566,946 - +0.8 566,981 + +1.0 566,982 - -0.5 566,982 - -0.4 566,982 - -0.9 566,982 - -0.7 567,011 + +0.1 567,060 - +1.1 567,067 - -1.5 567,067 - +1.8 567,067 - -0.0 567,067 - -1.2 567,202 - RR42_RS23340 0.13 +0.4 567,202 - RR42_RS23340 0.13 +0.4 567,497 + RR42_RS23340 0.37 -0.8 567,498 - RR42_RS23340 0.37 +0.1 567,652 + RR42_RS23340 0.50 +2.5 567,750 - RR42_RS23340 0.58 +0.0 567,886 + RR42_RS23340 0.69 +0.4 567,886 + RR42_RS23340 0.69 -1.2 567,887 - RR42_RS23340 0.69 -0.1 567,887 - RR42_RS23340 0.69 -0.7 567,887 - RR42_RS23340 0.69 -1.9 567,890 + RR42_RS23340 0.69 -1.7 567,890 + RR42_RS23340 0.69 -0.1 567,890 + RR42_RS23340 0.69 +1.5 568,005 - RR42_RS23340 0.78 +1.9 568,005 - RR42_RS23340 0.78 +1.4 568,182 - +0.9 568,486 + RR42_RS23345 0.13 -0.9 568,667 + RR42_RS23345 0.35 +0.3 568,667 + RR42_RS23345 0.35 -1.9 568,667 + RR42_RS23345 0.35 -0.4 568,667 + RR42_RS23345 0.35 -1.4 568,668 - RR42_RS23345 0.35 +0.1 568,668 - RR42_RS23345 0.35 +0.2 568,668 - RR42_RS23345 0.35 +0.3 568,862 - RR42_RS23345 0.58 -1.5 569,202 - -0.1
Or see this region's nucleotide sequence