Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23290 and RR42_RS23295 are separated by 32 nucleotides RR42_RS23295 and RR42_RS23300 are separated by 29 nucleotides
RR42_RS23290: RR42_RS23290 - hypothetical protein, at 555,575 to 557,095
_RS23290
RR42_RS23295: RR42_RS23295 - hypothetical protein, at 557,128 to 557,865
_RS23295
RR42_RS23300: RR42_RS23300 - hypothetical protein, at 557,895 to 558,860
_RS23300
Position (kb) 557
558 Strain fitness (log2 ratio) -2
-1
0
1
2 at 556.475 kb on + strand, within RR42_RS23290 at 556.475 kb on + strand, within RR42_RS23290 at 556.484 kb on + strand, within RR42_RS23290 at 556.484 kb on + strand, within RR42_RS23290 at 556.485 kb on - strand, within RR42_RS23290 at 556.719 kb on - strand, within RR42_RS23290 at 556.734 kb on - strand, within RR42_RS23290 at 556.757 kb on + strand, within RR42_RS23290 at 557.093 kb on + strand at 557.094 kb on - strand at 557.100 kb on - strand at 557.127 kb on + strand at 557.127 kb on + strand at 557.128 kb on - strand at 557.128 kb on - strand at 557.128 kb on - strand at 557.514 kb on + strand, within RR42_RS23295 at 557.762 kb on - strand, within RR42_RS23295 at 557.762 kb on - strand, within RR42_RS23295 at 557.990 kb on + strand at 557.990 kb on + strand at 557.991 kb on - strand at 557.991 kb on - strand at 558.052 kb on + strand, within RR42_RS23300 at 558.053 kb on - strand, within RR42_RS23300 at 558.053 kb on - strand, within RR42_RS23300 at 558.392 kb on - strand, within RR42_RS23300 at 558.392 kb on - strand, within RR42_RS23300 at 558.392 kb on - strand, within RR42_RS23300 at 558.460 kb on - strand, within RR42_RS23300 at 558.460 kb on - strand, within RR42_RS23300 at 558.572 kb on - strand, within RR42_RS23300 at 558.623 kb on + strand, within RR42_RS23300 at 558.788 kb on + strand at 558.789 kb on - strand at 558.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 556,475 + RR42_RS23290 0.59 +2.0 556,475 + RR42_RS23290 0.59 -0.4 556,484 + RR42_RS23290 0.60 +0.7 556,484 + RR42_RS23290 0.60 -0.2 556,485 - RR42_RS23290 0.60 +0.3 556,719 - RR42_RS23290 0.75 +0.4 556,734 - RR42_RS23290 0.76 +1.3 556,757 + RR42_RS23290 0.78 -0.4 557,093 + -2.4 557,094 - +0.0 557,100 - -1.8 557,127 + +0.6 557,127 + -0.4 557,128 - -2.3 557,128 - +0.6 557,128 - -1.6 557,514 + RR42_RS23295 0.52 -1.2 557,762 - RR42_RS23295 0.86 +1.1 557,762 - RR42_RS23295 0.86 +0.3 557,990 + +1.4 557,990 + -2.4 557,991 - -0.8 557,991 - +0.6 558,052 + RR42_RS23300 0.16 +0.0 558,053 - RR42_RS23300 0.16 +0.1 558,053 - RR42_RS23300 0.16 -1.5 558,392 - RR42_RS23300 0.51 -0.1 558,392 - RR42_RS23300 0.51 -1.1 558,392 - RR42_RS23300 0.51 -1.7 558,460 - RR42_RS23300 0.58 +0.6 558,460 - RR42_RS23300 0.58 -1.0 558,572 - RR42_RS23300 0.70 +0.4 558,623 + RR42_RS23300 0.75 +0.9 558,788 + -0.9 558,789 - +0.0 558,793 - +0.7
Or see this region's nucleotide sequence