Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23100 and RR42_RS23105 are separated by 135 nucleotides RR42_RS23105 and RR42_RS23110 are separated by 193 nucleotides RR42_RS23110 and RR42_RS23115 are separated by 149 nucleotides
RR42_RS23100: RR42_RS23100 - AraC family transcriptional regulator, at 517,259 to 518,224
_RS23100
RR42_RS23105: RR42_RS23105 - hypothetical protein, at 518,360 to 518,818
_RS23105
RR42_RS23110: RR42_RS23110 - isochorismatase, at 519,012 to 519,542
_RS23110
RR42_RS23115: RR42_RS23115 - serine hydrolase, at 519,692 to 521,401
_RS23115
Position (kb) 519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 518.052 kb on - strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.092 kb on + strand, within RR42_RS23100 at 518.118 kb on + strand, within RR42_RS23100 at 518.118 kb on + strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.155 kb on + strand at 518.155 kb on + strand at 518.202 kb on - strand at 518.250 kb on + strand at 518.251 kb on - strand at 518.419 kb on - strand, within RR42_RS23105 at 518.419 kb on - strand, within RR42_RS23105 at 518.608 kb on + strand, within RR42_RS23105 at 518.608 kb on + strand, within RR42_RS23105 at 518.665 kb on - strand, within RR42_RS23105 at 518.665 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.704 kb on - strand, within RR42_RS23105 at 518.918 kb on + strand at 518.918 kb on + strand at 518.918 kb on + strand at 518.982 kb on - strand at 519.317 kb on - strand, within RR42_RS23110 at 519.363 kb on - strand, within RR42_RS23110 at 519.513 kb on + strand at 519.513 kb on + strand at 519.514 kb on - strand at 519.514 kb on - strand at 519.693 kb on + strand at 519.741 kb on + strand at 519.741 kb on + strand at 519.741 kb on + strand at 519.742 kb on - strand at 519.879 kb on - strand, within RR42_RS23115 at 519.879 kb on - strand, within RR42_RS23115 at 519.995 kb on + strand, within RR42_RS23115 at 519.995 kb on + strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.059 kb on + strand, within RR42_RS23115 at 520.128 kb on + strand, within RR42_RS23115 at 520.213 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.398 kb on + strand, within RR42_RS23115 at 520.398 kb on + strand, within RR42_RS23115 at 520.399 kb on - strand, within RR42_RS23115 at 520.536 kb on + strand, within RR42_RS23115
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 518,052 - RR42_RS23100 0.82 +0.2 518,052 - RR42_RS23100 0.82 +0.9 518,052 - RR42_RS23100 0.82 +1.5 518,092 + RR42_RS23100 0.86 +0.2 518,118 + RR42_RS23100 0.89 -0.9 518,118 + RR42_RS23100 0.89 +0.4 518,119 - RR42_RS23100 0.89 +1.6 518,119 - RR42_RS23100 0.89 +0.4 518,119 - RR42_RS23100 0.89 +0.9 518,155 + -0.1 518,155 + +0.0 518,202 - -0.3 518,250 + -2.4 518,251 - +0.2 518,419 - RR42_RS23105 0.13 -2.4 518,419 - RR42_RS23105 0.13 +0.5 518,608 + RR42_RS23105 0.54 -0.2 518,608 + RR42_RS23105 0.54 +0.6 518,665 - RR42_RS23105 0.66 -0.4 518,665 - RR42_RS23105 0.66 -1.9 518,671 - RR42_RS23105 0.68 -2.2 518,671 - RR42_RS23105 0.68 +2.4 518,671 - RR42_RS23105 0.68 +0.1 518,704 - RR42_RS23105 0.75 -0.2 518,918 + -2.9 518,918 + +1.4 518,918 + -0.3 518,982 - +2.5 519,317 - RR42_RS23110 0.57 -2.4 519,363 - RR42_RS23110 0.66 -0.5 519,513 + -0.9 519,513 + +0.5 519,514 - -1.3 519,514 - +0.5 519,693 + +0.6 519,741 + +1.8 519,741 + +1.9 519,741 + -1.4 519,742 - +1.9 519,879 - RR42_RS23115 0.11 -1.4 519,879 - RR42_RS23115 0.11 -1.1 519,995 + RR42_RS23115 0.18 +0.3 519,995 + RR42_RS23115 0.18 -1.3 520,001 - RR42_RS23115 0.18 +1.1 520,001 - RR42_RS23115 0.18 -0.7 520,001 - RR42_RS23115 0.18 -1.9 520,059 + RR42_RS23115 0.21 +0.1 520,128 + RR42_RS23115 0.25 +0.3 520,213 - RR42_RS23115 0.30 -0.4 520,240 - RR42_RS23115 0.32 -0.5 520,240 - RR42_RS23115 0.32 -0.4 520,240 - RR42_RS23115 0.32 +0.5 520,398 + RR42_RS23115 0.41 +1.1 520,398 + RR42_RS23115 0.41 +0.2 520,399 - RR42_RS23115 0.41 -0.9 520,536 + RR42_RS23115 0.49 -0.4
Or see this region's nucleotide sequence