Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23070 and RR42_RS23075 are separated by 31 nucleotides RR42_RS23075 and RR42_RS23080 are separated by 204 nucleotides
RR42_RS23070: RR42_RS23070 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 510,423 to 511,454
_RS23070
RR42_RS23075: RR42_RS23075 - ABC transporter substrate-binding protein, at 511,486 to 512,451
_RS23075
RR42_RS23080: RR42_RS23080 - LysR family transcriptional regulator, at 512,656 to 513,591
_RS23080
Position (kb) 511
512
513 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 510.634 kb on + strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 511.040 kb on + strand, within RR42_RS23070 at 511.154 kb on - strand, within RR42_RS23070 at 511.573 kb on - strand at 511.694 kb on + strand, within RR42_RS23075 at 511.695 kb on - strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.822 kb on - strand, within RR42_RS23075 at 511.907 kb on + strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.993 kb on - strand, within RR42_RS23075 at 512.221 kb on - strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.385 kb on - strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.470 kb on + strand at 512.526 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.537 kb on + strand at 512.537 kb on + strand at 512.836 kb on + strand, within RR42_RS23080 at 512.837 kb on - strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.084 kb on - strand, within RR42_RS23080 at 513.090 kb on - strand, within RR42_RS23080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 510,634 + RR42_RS23070 0.20 +0.4 510,635 - RR42_RS23070 0.21 +0.6 510,635 - RR42_RS23070 0.21 -0.0 510,635 - RR42_RS23070 0.21 +1.9 510,635 - RR42_RS23070 0.21 -0.1 511,040 + RR42_RS23070 0.60 +0.1 511,154 - RR42_RS23070 0.71 -2.5 511,573 - -0.9 511,694 + RR42_RS23075 0.22 +2.5 511,695 - RR42_RS23075 0.22 +0.6 511,821 + RR42_RS23075 0.35 +0.3 511,821 + RR42_RS23075 0.35 -1.7 511,822 - RR42_RS23075 0.35 +0.7 511,907 + RR42_RS23075 0.44 +0.8 511,908 - RR42_RS23075 0.44 +0.1 511,908 - RR42_RS23075 0.44 +0.5 511,992 + RR42_RS23075 0.52 -0.3 511,992 + RR42_RS23075 0.52 +1.0 511,992 + RR42_RS23075 0.52 -0.4 511,992 + RR42_RS23075 0.52 -1.1 511,993 - RR42_RS23075 0.52 +0.8 512,221 - RR42_RS23075 0.76 +0.9 512,235 + RR42_RS23075 0.78 -0.7 512,235 + RR42_RS23075 0.78 -2.3 512,236 - RR42_RS23075 0.78 -0.3 512,236 - RR42_RS23075 0.78 -1.0 512,236 - RR42_RS23075 0.78 -0.6 512,385 - -0.9 512,452 + -0.1 512,452 + -0.7 512,452 + -0.4 512,470 + +1.0 512,526 + +0.4 512,535 + -0.9 512,535 + -1.4 512,535 + -0.6 512,535 + -0.1 512,536 - -0.0 512,536 - -0.5 512,536 - -0.1 512,536 - -0.3 512,536 - -3.8 512,537 + -0.3 512,537 + +0.1 512,836 + RR42_RS23080 0.19 -0.2 512,837 - RR42_RS23080 0.19 +0.1 513,083 + RR42_RS23080 0.46 -1.0 513,083 + RR42_RS23080 0.46 +0.8 513,084 - RR42_RS23080 0.46 +0.5 513,090 - RR42_RS23080 0.46 +0.4
Or see this region's nucleotide sequence