Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23060 and RR42_RS23065 are separated by 158 nucleotides RR42_RS23065 and RR42_RS23070 are separated by 162 nucleotides
RR42_RS23060: RR42_RS23060 - hypothetical protein, at 507,934 to 508,926
_RS23060
RR42_RS23065: RR42_RS23065 - L-rhamnose 1-epimerase, at 509,085 to 510,260
_RS23065
RR42_RS23070: RR42_RS23070 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 510,423 to 511,454
_RS23070
Position (kb) 509
510
511 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 508.282 kb on + strand, within RR42_RS23060 at 508.294 kb on + strand, within RR42_RS23060 at 508.294 kb on + strand, within RR42_RS23060 at 508.363 kb on + strand, within RR42_RS23060 at 508.363 kb on + strand, within RR42_RS23060 at 508.396 kb on + strand, within RR42_RS23060 at 508.495 kb on - strand, within RR42_RS23060 at 508.642 kb on + strand, within RR42_RS23060 at 508.832 kb on - strand at 508.832 kb on - strand at 508.832 kb on - strand at 508.892 kb on + strand at 508.892 kb on + strand at 508.892 kb on + strand at 509.093 kb on + strand at 509.093 kb on + strand at 509.113 kb on + strand at 509.114 kb on - strand at 509.114 kb on - strand at 509.167 kb on + strand at 509.168 kb on - strand at 509.203 kb on + strand, within RR42_RS23065 at 509.204 kb on - strand, within RR42_RS23065 at 509.204 kb on - strand, within RR42_RS23065 at 509.204 kb on - strand, within RR42_RS23065 at 509.204 kb on - strand, within RR42_RS23065 at 509.206 kb on + strand, within RR42_RS23065 at 509.206 kb on + strand, within RR42_RS23065 at 509.290 kb on + strand, within RR42_RS23065 at 509.290 kb on + strand, within RR42_RS23065 at 509.290 kb on + strand, within RR42_RS23065 at 509.290 kb on + strand, within RR42_RS23065 at 509.291 kb on - strand, within RR42_RS23065 at 509.291 kb on - strand, within RR42_RS23065 at 509.395 kb on + strand, within RR42_RS23065 at 509.443 kb on + strand, within RR42_RS23065 at 509.443 kb on + strand, within RR42_RS23065 at 509.506 kb on + strand, within RR42_RS23065 at 509.609 kb on + strand, within RR42_RS23065 at 509.610 kb on - strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.755 kb on + strand, within RR42_RS23065 at 509.755 kb on + strand, within RR42_RS23065 at 509.756 kb on - strand, within RR42_RS23065 at 509.801 kb on - strand, within RR42_RS23065 at 509.801 kb on - strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.816 kb on - strand, within RR42_RS23065 at 509.816 kb on - strand, within RR42_RS23065 at 509.816 kb on - strand, within RR42_RS23065 at 509.965 kb on + strand, within RR42_RS23065 at 509.965 kb on + strand, within RR42_RS23065 at 509.966 kb on - strand, within RR42_RS23065 at 509.983 kb on + strand, within RR42_RS23065 at 509.984 kb on - strand, within RR42_RS23065 at 509.984 kb on - strand, within RR42_RS23065 at 510.028 kb on + strand, within RR42_RS23065 at 510.029 kb on - strand, within RR42_RS23065 at 510.029 kb on - strand, within RR42_RS23065 at 510.424 kb on + strand at 510.634 kb on + strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 511.040 kb on + strand, within RR42_RS23070 at 511.154 kb on - strand, within RR42_RS23070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 508,282 + RR42_RS23060 0.35 -0.1 508,294 + RR42_RS23060 0.36 -1.7 508,294 + RR42_RS23060 0.36 +0.0 508,363 + RR42_RS23060 0.43 -1.3 508,363 + RR42_RS23060 0.43 -0.8 508,396 + RR42_RS23060 0.47 +0.8 508,495 - RR42_RS23060 0.56 -0.4 508,642 + RR42_RS23060 0.71 -2.1 508,832 - +1.8 508,832 - -0.3 508,832 - +0.0 508,892 + -0.2 508,892 + -0.3 508,892 + +0.7 509,093 + +1.6 509,093 + +0.6 509,113 + +0.6 509,114 - -0.4 509,114 - +0.0 509,167 + +0.6 509,168 - -1.6 509,203 + RR42_RS23065 0.10 +0.3 509,204 - RR42_RS23065 0.10 +1.4 509,204 - RR42_RS23065 0.10 -1.2 509,204 - RR42_RS23065 0.10 +0.1 509,204 - RR42_RS23065 0.10 +1.1 509,206 + RR42_RS23065 0.10 -1.2 509,206 + RR42_RS23065 0.10 -0.5 509,290 + RR42_RS23065 0.17 -0.1 509,290 + RR42_RS23065 0.17 +0.8 509,290 + RR42_RS23065 0.17 +2.5 509,290 + RR42_RS23065 0.17 +0.4 509,291 - RR42_RS23065 0.18 -1.9 509,291 - RR42_RS23065 0.18 +0.6 509,395 + RR42_RS23065 0.26 -2.6 509,443 + RR42_RS23065 0.30 +0.2 509,443 + RR42_RS23065 0.30 -0.4 509,506 + RR42_RS23065 0.36 +0.8 509,609 + RR42_RS23065 0.45 -1.4 509,610 - RR42_RS23065 0.45 -0.6 509,611 + RR42_RS23065 0.45 -0.4 509,611 + RR42_RS23065 0.45 +0.2 509,611 + RR42_RS23065 0.45 +1.0 509,611 + RR42_RS23065 0.45 +1.0 509,611 + RR42_RS23065 0.45 +0.9 509,612 - RR42_RS23065 0.45 -3.0 509,612 - RR42_RS23065 0.45 -1.2 509,612 - RR42_RS23065 0.45 -0.2 509,612 - RR42_RS23065 0.45 -1.4 509,612 - RR42_RS23065 0.45 +0.8 509,612 - RR42_RS23065 0.45 +1.0 509,612 - RR42_RS23065 0.45 -1.8 509,612 - RR42_RS23065 0.45 -1.5 509,755 + RR42_RS23065 0.57 -0.0 509,755 + RR42_RS23065 0.57 -0.1 509,756 - RR42_RS23065 0.57 -3.4 509,801 - RR42_RS23065 0.61 +0.4 509,801 - RR42_RS23065 0.61 -1.3 509,815 + RR42_RS23065 0.62 +0.6 509,815 + RR42_RS23065 0.62 +1.0 509,815 + RR42_RS23065 0.62 -0.2 509,815 + RR42_RS23065 0.62 -1.2 509,816 - RR42_RS23065 0.62 -2.4 509,816 - RR42_RS23065 0.62 +1.2 509,816 - RR42_RS23065 0.62 +0.4 509,965 + RR42_RS23065 0.75 +0.2 509,965 + RR42_RS23065 0.75 +1.9 509,966 - RR42_RS23065 0.75 -1.6 509,983 + RR42_RS23065 0.76 +0.8 509,984 - RR42_RS23065 0.76 +0.5 509,984 - RR42_RS23065 0.76 -1.8 510,028 + RR42_RS23065 0.80 -0.1 510,029 - RR42_RS23065 0.80 +1.3 510,029 - RR42_RS23065 0.80 -2.3 510,424 + -2.1 510,634 + RR42_RS23070 0.20 +0.4 510,635 - RR42_RS23070 0.21 +0.6 510,635 - RR42_RS23070 0.21 -0.0 510,635 - RR42_RS23070 0.21 +1.9 510,635 - RR42_RS23070 0.21 -0.1 511,040 + RR42_RS23070 0.60 +0.1 511,154 - RR42_RS23070 0.71 -2.5
Or see this region's nucleotide sequence