Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22795 and RR42_RS22800 are separated by 30 nucleotides RR42_RS22800 and RR42_RS22805 are separated by 248 nucleotides
RR42_RS22795: RR42_RS22795 - hypothetical protein, at 446,477 to 447,442
_RS22795
RR42_RS22800: RR42_RS22800 - DSBA oxidoreductase, at 447,473 to 449,029
_RS22800
RR42_RS22805: RR42_RS22805 - LysR family transcriptional regulator, at 449,278 to 450,153
_RS22805
Position (kb) 447
448
449
450 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 446.728 kb on + strand, within RR42_RS22795 at 446.728 kb on + strand, within RR42_RS22795 at 446.728 kb on + strand, within RR42_RS22795 at 446.729 kb on - strand, within RR42_RS22795 at 446.729 kb on - strand, within RR42_RS22795 at 446.729 kb on - strand, within RR42_RS22795 at 447.017 kb on + strand, within RR42_RS22795 at 447.017 kb on + strand, within RR42_RS22795 at 447.018 kb on - strand, within RR42_RS22795 at 447.230 kb on + strand, within RR42_RS22795 at 447.230 kb on + strand, within RR42_RS22795 at 447.231 kb on - strand, within RR42_RS22795 at 447.231 kb on - strand, within RR42_RS22795 at 447.231 kb on - strand, within RR42_RS22795 at 447.231 kb on - strand, within RR42_RS22795 at 447.787 kb on - strand, within RR42_RS22800 at 447.972 kb on + strand, within RR42_RS22800 at 447.972 kb on + strand, within RR42_RS22800 at 447.972 kb on + strand, within RR42_RS22800 at 448.170 kb on + strand, within RR42_RS22800 at 448.170 kb on + strand, within RR42_RS22800 at 448.171 kb on - strand, within RR42_RS22800 at 448.171 kb on - strand, within RR42_RS22800 at 448.171 kb on - strand, within RR42_RS22800 at 448.172 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.358 kb on - strand, within RR42_RS22800 at 448.447 kb on + strand, within RR42_RS22800 at 448.447 kb on + strand, within RR42_RS22800 at 448.448 kb on - strand, within RR42_RS22800 at 448.481 kb on - strand, within RR42_RS22800 at 448.875 kb on + strand at 449.243 kb on - strand at 449.373 kb on + strand, within RR42_RS22805 at 449.566 kb on + strand, within RR42_RS22805 at 449.713 kb on + strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.715 kb on + strand, within RR42_RS22805 at 449.715 kb on + strand, within RR42_RS22805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 446,728 + RR42_RS22795 0.26 +0.6 446,728 + RR42_RS22795 0.26 -0.4 446,728 + RR42_RS22795 0.26 -2.5 446,729 - RR42_RS22795 0.26 +0.7 446,729 - RR42_RS22795 0.26 -0.2 446,729 - RR42_RS22795 0.26 +2.0 447,017 + RR42_RS22795 0.56 -0.7 447,017 + RR42_RS22795 0.56 -0.1 447,018 - RR42_RS22795 0.56 +0.5 447,230 + RR42_RS22795 0.78 -0.9 447,230 + RR42_RS22795 0.78 -2.5 447,231 - RR42_RS22795 0.78 -0.2 447,231 - RR42_RS22795 0.78 +0.5 447,231 - RR42_RS22795 0.78 +0.6 447,231 - RR42_RS22795 0.78 -1.2 447,787 - RR42_RS22800 0.20 -0.4 447,972 + RR42_RS22800 0.32 -0.4 447,972 + RR42_RS22800 0.32 -0.0 447,972 + RR42_RS22800 0.32 +0.4 448,170 + RR42_RS22800 0.45 -0.9 448,170 + RR42_RS22800 0.45 +1.2 448,171 - RR42_RS22800 0.45 -0.1 448,171 - RR42_RS22800 0.45 +2.2 448,171 - RR42_RS22800 0.45 -1.6 448,172 + RR42_RS22800 0.45 -0.3 448,357 + RR42_RS22800 0.57 +2.6 448,357 + RR42_RS22800 0.57 +0.4 448,357 + RR42_RS22800 0.57 -0.4 448,358 - RR42_RS22800 0.57 -0.1 448,447 + RR42_RS22800 0.63 +0.6 448,447 + RR42_RS22800 0.63 -0.4 448,448 - RR42_RS22800 0.63 -0.7 448,481 - RR42_RS22800 0.65 +0.0 448,875 + -1.4 449,243 - +0.2 449,373 + RR42_RS22805 0.11 +0.3 449,566 + RR42_RS22805 0.33 +0.6 449,713 + RR42_RS22805 0.50 +0.9 449,714 - RR42_RS22805 0.50 +0.4 449,714 - RR42_RS22805 0.50 +1.2 449,714 - RR42_RS22805 0.50 +0.6 449,714 - RR42_RS22805 0.50 -1.3 449,715 + RR42_RS22805 0.50 +2.0 449,715 + RR42_RS22805 0.50 -2.9
Or see this region's nucleotide sequence