Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22745 and RR42_RS22750 are separated by 100 nucleotides RR42_RS22750 and RR42_RS22755 are separated by 67 nucleotides RR42_RS22755 and RR42_RS22760 are separated by 43 nucleotides
RR42_RS22745: RR42_RS22745 - phosphoribosylglycinamide formyltransferase, at 436,789 to 437,949
_RS22745
RR42_RS22750: RR42_RS22750 - aspartate racemase, at 438,050 to 438,748
_RS22750
RR42_RS22755: RR42_RS22755 - hypothetical protein, at 438,816 to 439,196
_RS22755
RR42_RS22760: RR42_RS22760 - GntR family transcriptional regulator, at 439,240 to 440,004
_RS22760
Position (kb) 438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 437.140 kb on + strand, within RR42_RS22745 at 437.287 kb on + strand, within RR42_RS22745 at 437.288 kb on - strand, within RR42_RS22745 at 437.353 kb on + strand, within RR42_RS22745 at 437.740 kb on + strand, within RR42_RS22745 at 437.740 kb on + strand, within RR42_RS22745 at 437.740 kb on + strand, within RR42_RS22745 at 437.740 kb on + strand, within RR42_RS22745 at 437.741 kb on - strand, within RR42_RS22745 at 437.959 kb on - strand at 437.969 kb on - strand at 438.061 kb on + strand at 438.062 kb on - strand at 438.062 kb on - strand at 438.163 kb on - strand, within RR42_RS22750 at 438.191 kb on + strand, within RR42_RS22750 at 438.228 kb on + strand, within RR42_RS22750 at 438.228 kb on + strand, within RR42_RS22750 at 438.228 kb on + strand, within RR42_RS22750 at 438.323 kb on + strand, within RR42_RS22750 at 438.323 kb on + strand, within RR42_RS22750 at 438.323 kb on + strand, within RR42_RS22750 at 438.323 kb on + strand, within RR42_RS22750 at 438.324 kb on - strand, within RR42_RS22750 at 438.358 kb on + strand, within RR42_RS22750 at 438.359 kb on - strand, within RR42_RS22750 at 438.668 kb on - strand, within RR42_RS22750 at 438.744 kb on - strand at 438.776 kb on + strand at 438.776 kb on + strand at 438.776 kb on + strand at 438.776 kb on + strand at 438.776 kb on + strand at 438.776 kb on + strand at 438.777 kb on - strand at 438.779 kb on + strand at 438.779 kb on + strand at 438.779 kb on + strand at 438.780 kb on - strand at 438.780 kb on - strand at 438.970 kb on - strand, within RR42_RS22755 at 438.970 kb on - strand, within RR42_RS22755 at 438.992 kb on + strand, within RR42_RS22755 at 438.992 kb on + strand, within RR42_RS22755 at 438.993 kb on - strand, within RR42_RS22755 at 438.993 kb on - strand, within RR42_RS22755 at 439.026 kb on + strand, within RR42_RS22755 at 439.036 kb on - strand, within RR42_RS22755 at 439.036 kb on - strand, within RR42_RS22755 at 439.053 kb on + strand, within RR42_RS22755 at 439.056 kb on + strand, within RR42_RS22755 at 439.056 kb on + strand, within RR42_RS22755 at 439.057 kb on - strand, within RR42_RS22755 at 439.057 kb on - strand, within RR42_RS22755 at 439.057 kb on - strand, within RR42_RS22755 at 439.057 kb on - strand, within RR42_RS22755 at 439.057 kb on - strand, within RR42_RS22755 at 439.057 kb on - strand, within RR42_RS22755 at 439.104 kb on + strand, within RR42_RS22755 at 439.105 kb on - strand, within RR42_RS22755 at 439.105 kb on - strand, within RR42_RS22755 at 439.105 kb on - strand, within RR42_RS22755 at 439.155 kb on + strand, within RR42_RS22755 at 439.155 kb on + strand, within RR42_RS22755 at 439.223 kb on + strand at 439.223 kb on + strand at 439.224 kb on - strand at 439.224 kb on - strand at 439.265 kb on - strand at 439.305 kb on + strand at 439.306 kb on - strand at 439.306 kb on - strand at 439.306 kb on - strand at 439.340 kb on + strand, within RR42_RS22760 at 439.340 kb on + strand, within RR42_RS22760 at 439.341 kb on - strand, within RR42_RS22760 at 439.341 kb on - strand, within RR42_RS22760 at 439.341 kb on - strand, within RR42_RS22760 at 439.341 kb on - strand, within RR42_RS22760 at 439.341 kb on - strand, within RR42_RS22760 at 439.352 kb on + strand, within RR42_RS22760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 437,140 + RR42_RS22745 0.30 +0.0 437,287 + RR42_RS22745 0.43 -0.4 437,288 - RR42_RS22745 0.43 -0.3 437,353 + RR42_RS22745 0.49 -1.4 437,740 + RR42_RS22745 0.82 -0.8 437,740 + RR42_RS22745 0.82 +0.8 437,740 + RR42_RS22745 0.82 +0.1 437,740 + RR42_RS22745 0.82 +0.2 437,741 - RR42_RS22745 0.82 +1.3 437,959 - -2.2 437,969 - -1.5 438,061 + +0.8 438,062 - +0.8 438,062 - -2.2 438,163 - RR42_RS22750 0.16 -0.5 438,191 + RR42_RS22750 0.20 -1.6 438,228 + RR42_RS22750 0.25 +1.3 438,228 + RR42_RS22750 0.25 -1.8 438,228 + RR42_RS22750 0.25 +1.4 438,323 + RR42_RS22750 0.39 +0.5 438,323 + RR42_RS22750 0.39 -0.2 438,323 + RR42_RS22750 0.39 -0.3 438,323 + RR42_RS22750 0.39 +0.9 438,324 - RR42_RS22750 0.39 +1.1 438,358 + RR42_RS22750 0.44 -1.2 438,359 - RR42_RS22750 0.44 +0.3 438,668 - RR42_RS22750 0.88 +0.6 438,744 - -2.2 438,776 + -0.6 438,776 + -0.7 438,776 + -0.3 438,776 + +0.5 438,776 + -0.4 438,776 + +0.4 438,777 - +0.6 438,779 + -0.6 438,779 + +1.8 438,779 + -0.2 438,780 - +1.2 438,780 - -1.0 438,970 - RR42_RS22755 0.40 -0.8 438,970 - RR42_RS22755 0.40 +0.1 438,992 + RR42_RS22755 0.46 +0.2 438,992 + RR42_RS22755 0.46 +1.9 438,993 - RR42_RS22755 0.46 +1.2 438,993 - RR42_RS22755 0.46 -1.4 439,026 + RR42_RS22755 0.55 +0.7 439,036 - RR42_RS22755 0.58 -0.6 439,036 - RR42_RS22755 0.58 -2.6 439,053 + RR42_RS22755 0.62 -0.2 439,056 + RR42_RS22755 0.63 -2.0 439,056 + RR42_RS22755 0.63 -1.2 439,057 - RR42_RS22755 0.63 -3.5 439,057 - RR42_RS22755 0.63 -0.2 439,057 - RR42_RS22755 0.63 -2.0 439,057 - RR42_RS22755 0.63 +0.1 439,057 - RR42_RS22755 0.63 -1.1 439,057 - RR42_RS22755 0.63 +0.2 439,104 + RR42_RS22755 0.76 -0.1 439,105 - RR42_RS22755 0.76 -0.8 439,105 - RR42_RS22755 0.76 -0.9 439,105 - RR42_RS22755 0.76 +0.3 439,155 + RR42_RS22755 0.89 -0.6 439,155 + RR42_RS22755 0.89 +0.0 439,223 + -0.6 439,223 + -0.2 439,224 - +0.3 439,224 - -0.2 439,265 - -0.2 439,305 + +0.8 439,306 - +0.7 439,306 - +1.9 439,306 - -0.3 439,340 + RR42_RS22760 0.13 +0.3 439,340 + RR42_RS22760 0.13 -2.5 439,341 - RR42_RS22760 0.13 -0.3 439,341 - RR42_RS22760 0.13 +0.5 439,341 - RR42_RS22760 0.13 +0.9 439,341 - RR42_RS22760 0.13 -0.1 439,341 - RR42_RS22760 0.13 -0.7 439,352 + RR42_RS22760 0.15 -1.7
Or see this region's nucleotide sequence