Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22700 and RR42_RS22705 are separated by 506 nucleotides RR42_RS22705 and RR42_RS22710 are separated by 86 nucleotides RR42_RS22710 and RR42_RS22715 are separated by 225 nucleotides
RR42_RS22700: RR42_RS22700 - phasin, at 427,518 to 428,075
_RS22700
RR42_RS22705: RR42_RS22705 - oxidoreductase, at 428,582 to 428,905
_RS22705
RR42_RS22710: RR42_RS22710 - short-chain dehydrogenase, at 428,992 to 429,741
_RS22710
RR42_RS22715: RR42_RS22715 - AMP-binding protein, at 429,967 to 431,616
_RS22715
Position (kb) 428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 428.000 kb on + strand, within RR42_RS22700 at 428.001 kb on - strand, within RR42_RS22700 at 428.001 kb on - strand, within RR42_RS22700 at 428.091 kb on + strand at 428.213 kb on + strand at 428.223 kb on + strand at 428.263 kb on + strand at 428.264 kb on - strand at 428.330 kb on + strand at 428.330 kb on + strand at 428.331 kb on - strand at 428.410 kb on - strand at 428.477 kb on - strand at 428.583 kb on + strand at 428.583 kb on + strand at 428.670 kb on + strand, within RR42_RS22705 at 428.695 kb on + strand, within RR42_RS22705 at 428.712 kb on + strand, within RR42_RS22705 at 428.712 kb on + strand, within RR42_RS22705 at 428.713 kb on - strand, within RR42_RS22705 at 428.752 kb on + strand, within RR42_RS22705 at 428.781 kb on + strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.885 kb on - strand at 428.940 kb on + strand at 428.940 kb on + strand at 428.940 kb on + strand at 428.941 kb on - strand at 428.941 kb on - strand at 428.941 kb on - strand at 428.949 kb on + strand at 428.950 kb on - strand at 428.966 kb on + strand at 428.967 kb on - strand at 428.967 kb on - strand at 429.180 kb on - strand, within RR42_RS22710 at 429.293 kb on + strand, within RR42_RS22710 at 429.782 kb on + strand at 429.782 kb on + strand at 429.782 kb on + strand at 429.801 kb on + strand at 429.968 kb on + strand at 429.969 kb on - strand at 430.080 kb on - strand at 430.148 kb on + strand, within RR42_RS22715 at 430.317 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.370 kb on + strand, within RR42_RS22715 at 430.371 kb on - strand, within RR42_RS22715 at 430.441 kb on - strand, within RR42_RS22715 at 430.604 kb on + strand, within RR42_RS22715
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 428,000 + RR42_RS22700 0.86 +0.4 428,001 - RR42_RS22700 0.87 +0.7 428,001 - RR42_RS22700 0.87 -2.3 428,091 + -1.2 428,213 + -0.3 428,223 + +1.7 428,263 + +0.2 428,264 - +0.1 428,330 + -0.2 428,330 + -1.4 428,331 - +0.7 428,410 - -0.4 428,477 - -2.8 428,583 + -3.0 428,583 + -0.8 428,670 + RR42_RS22705 0.27 -0.0 428,695 + RR42_RS22705 0.35 +0.1 428,712 + RR42_RS22705 0.40 -0.0 428,712 + RR42_RS22705 0.40 -2.0 428,713 - RR42_RS22705 0.40 +0.3 428,752 + RR42_RS22705 0.52 +1.5 428,781 + RR42_RS22705 0.61 +1.2 428,782 - RR42_RS22705 0.62 +0.1 428,782 - RR42_RS22705 0.62 -0.1 428,782 - RR42_RS22705 0.62 +1.2 428,782 - RR42_RS22705 0.62 -1.4 428,885 - -0.4 428,940 + -0.7 428,940 + -1.4 428,940 + -0.8 428,941 - -0.1 428,941 - +0.3 428,941 - -1.5 428,949 + +1.3 428,950 - -1.2 428,966 + +0.5 428,967 - -3.1 428,967 - -0.9 429,180 - RR42_RS22710 0.25 -1.1 429,293 + RR42_RS22710 0.40 -1.2 429,782 + -2.0 429,782 + +1.7 429,782 + +1.4 429,801 + -1.6 429,968 + -2.5 429,969 - +0.0 430,080 - +0.0 430,148 + RR42_RS22715 0.11 -1.0 430,317 + RR42_RS22715 0.21 -2.2 430,367 + RR42_RS22715 0.24 -0.6 430,367 + RR42_RS22715 0.24 +1.0 430,368 - RR42_RS22715 0.24 +1.0 430,368 - RR42_RS22715 0.24 +0.5 430,370 + RR42_RS22715 0.24 +0.6 430,371 - RR42_RS22715 0.24 -3.0 430,441 - RR42_RS22715 0.29 -0.0 430,604 + RR42_RS22715 0.39 -0.3
Or see this region's nucleotide sequence