Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22655 and RR42_RS22660 are separated by 56 nucleotides RR42_RS22660 and RR42_RS22665 are separated by 176 nucleotides RR42_RS22665 and RR42_RS22670 are separated by 375 nucleotides
RR42_RS22655: RR42_RS22655 - mandelate racemase, at 416,656 to 417,813
_RS22655
RR42_RS22660: RR42_RS22660 - hypothetical protein, at 417,870 to 418,847
_RS22660
RR42_RS22665: RR42_RS22665 - hypothetical protein, at 419,024 to 419,311
_RS22665
RR42_RS22670: RR42_RS22670 - penicillin acylase, at 419,687 to 422,188
_RS22670
Position (kb) 417
418
419 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 417.109 kb on + strand at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.110 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.460 kb on - strand, within RR42_RS22655 at 417.856 kb on + strand at 417.856 kb on + strand at 417.893 kb on - strand at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.173 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.252 kb on - strand, within RR42_RS22660 at 418.308 kb on + strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.417 kb on - strand, within RR42_RS22660 at 418.425 kb on + strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.583 kb on + strand, within RR42_RS22660 at 418.907 kb on + strand at 418.908 kb on - strand at 418.911 kb on - strand at 418.921 kb on + strand at 418.922 kb on - strand at 418.922 kb on - strand at 418.922 kb on - strand at 418.947 kb on - strand at 418.947 kb on - strand at 418.947 kb on - strand at 419.036 kb on + strand at 419.036 kb on + strand at 419.037 kb on - strand at 419.037 kb on - strand at 419.162 kb on + strand, within RR42_RS22665 at 419.162 kb on + strand, within RR42_RS22665 at 419.162 kb on + strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.357 kb on - strand at 419.415 kb on - strand at 419.536 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.790 kb on - strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.802 kb on - strand at 419.802 kb on - strand at 419.807 kb on + strand at 419.807 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 417,109 + +1.2 417,109 + RR42_RS22655 0.39 +0.1 417,109 + RR42_RS22655 0.39 +0.6 417,109 + RR42_RS22655 0.39 -1.0 417,109 + RR42_RS22655 0.39 -1.1 417,110 - RR42_RS22655 0.39 -0.1 417,136 - RR42_RS22655 0.41 -0.6 417,136 - RR42_RS22655 0.41 -3.8 417,460 - RR42_RS22655 0.69 -1.7 417,856 + -1.8 417,856 + -1.4 417,893 - -2.6 418,137 + RR42_RS22660 0.27 +0.6 418,137 + RR42_RS22660 0.27 +1.7 418,137 + RR42_RS22660 0.27 +0.0 418,173 + RR42_RS22660 0.31 -0.9 418,185 + RR42_RS22660 0.32 +0.5 418,185 + RR42_RS22660 0.32 +0.7 418,186 - RR42_RS22660 0.32 -0.6 418,186 - RR42_RS22660 0.32 +1.3 418,251 + RR42_RS22660 0.39 +0.3 418,251 + RR42_RS22660 0.39 -2.5 418,252 - RR42_RS22660 0.39 -0.8 418,308 + RR42_RS22660 0.45 -1.2 418,309 - RR42_RS22660 0.45 +0.0 418,309 - RR42_RS22660 0.45 +0.0 418,339 - RR42_RS22660 0.48 -0.9 418,339 - RR42_RS22660 0.48 -0.1 418,416 + RR42_RS22660 0.56 +0.4 418,416 + RR42_RS22660 0.56 -1.8 418,416 + RR42_RS22660 0.56 -0.0 418,416 + RR42_RS22660 0.56 +1.5 418,417 - RR42_RS22660 0.56 +0.0 418,425 + RR42_RS22660 0.57 -1.5 418,426 - RR42_RS22660 0.57 -0.3 418,426 - RR42_RS22660 0.57 +0.2 418,510 - RR42_RS22660 0.65 +1.4 418,510 - RR42_RS22660 0.65 -0.4 418,510 - RR42_RS22660 0.65 -0.2 418,510 - RR42_RS22660 0.65 +0.1 418,583 + RR42_RS22660 0.73 +0.2 418,907 + +0.5 418,908 - -0.7 418,911 - -0.3 418,921 + +0.6 418,922 - +0.1 418,922 - -2.7 418,922 - +0.6 418,947 - +0.9 418,947 - -0.5 418,947 - -1.1 419,036 + +1.4 419,036 + -0.6 419,037 - -1.9 419,037 - +0.4 419,162 + RR42_RS22665 0.48 -0.6 419,162 + RR42_RS22665 0.48 -0.8 419,162 + RR42_RS22665 0.48 +1.0 419,163 - RR42_RS22665 0.48 -0.7 419,163 - RR42_RS22665 0.48 +0.4 419,163 - RR42_RS22665 0.48 +1.3 419,163 - RR42_RS22665 0.48 +1.0 419,163 - RR42_RS22665 0.48 +0.0 419,357 - -0.5 419,415 - +0.1 419,536 - -0.3 419,775 - +2.4 419,775 - -0.9 419,775 - -0.4 419,775 - -2.9 419,775 - -1.3 419,790 - +0.7 419,801 + -0.4 419,801 + -0.1 419,801 + -2.1 419,801 + +0.8 419,801 + +1.4 419,801 + -0.6 419,802 - +0.3 419,802 - -0.7 419,807 + +0.2 419,807 + +0.9
Or see this region's nucleotide sequence