Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22640 and RR42_RS22645 are separated by 344 nucleotides RR42_RS22645 and RR42_RS22650 are separated by 230 nucleotides
RR42_RS22640: RR42_RS22640 - CAAX amino protease, at 413,175 to 413,963
_RS22640
RR42_RS22645: RR42_RS22645 - GntR family transcriptional regulator, at 414,308 to 415,360
_RS22645
RR42_RS22650: RR42_RS22650 - ABC transporter substrate-binding protein, at 415,591 to 416,580
_RS22650
Position (kb) 414
415
416 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 413.392 kb on + strand, within RR42_RS22640 at 413.392 kb on + strand, within RR42_RS22640 at 413.393 kb on - strand, within RR42_RS22640 at 413.440 kb on + strand, within RR42_RS22640 at 413.441 kb on - strand, within RR42_RS22640 at 413.441 kb on - strand, within RR42_RS22640 at 413.480 kb on - strand, within RR42_RS22640 at 413.780 kb on - strand, within RR42_RS22640 at 413.780 kb on - strand, within RR42_RS22640 at 413.824 kb on + strand, within RR42_RS22640 at 413.908 kb on - strand at 414.010 kb on + strand at 414.010 kb on + strand at 414.033 kb on - strand at 414.074 kb on + strand at 414.172 kb on + strand at 414.203 kb on - strand at 414.223 kb on - strand at 414.381 kb on + strand at 414.381 kb on + strand at 414.381 kb on + strand at 414.381 kb on + strand at 414.382 kb on - strand at 414.476 kb on + strand, within RR42_RS22645 at 414.476 kb on + strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.869 kb on + strand, within RR42_RS22645 at 415.388 kb on + strand at 415.527 kb on + strand at 415.527 kb on + strand at 415.687 kb on + strand at 415.688 kb on - strand at 415.870 kb on + strand, within RR42_RS22650 at 415.871 kb on - strand, within RR42_RS22650 at 415.912 kb on + strand, within RR42_RS22650 at 415.913 kb on - strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.159 kb on - strand, within RR42_RS22650 at 416.277 kb on - strand, within RR42_RS22650 at 416.302 kb on + strand, within RR42_RS22650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 413,392 + RR42_RS22640 0.28 +0.4 413,392 + RR42_RS22640 0.28 -2.1 413,393 - RR42_RS22640 0.28 +0.3 413,440 + RR42_RS22640 0.34 +1.7 413,441 - RR42_RS22640 0.34 -0.1 413,441 - RR42_RS22640 0.34 -0.4 413,480 - RR42_RS22640 0.39 +1.5 413,780 - RR42_RS22640 0.77 -0.4 413,780 - RR42_RS22640 0.77 +0.9 413,824 + RR42_RS22640 0.82 +1.8 413,908 - -0.3 414,010 + -2.4 414,010 + -1.2 414,033 - -0.3 414,074 + +1.1 414,172 + -1.3 414,203 - +1.8 414,223 - -0.3 414,381 + -2.4 414,381 + +0.3 414,381 + -2.5 414,381 + +0.1 414,382 - -0.9 414,476 + RR42_RS22645 0.16 +1.2 414,476 + RR42_RS22645 0.16 +0.8 414,782 - RR42_RS22645 0.45 -0.4 414,782 - RR42_RS22645 0.45 +2.3 414,782 - RR42_RS22645 0.45 -0.6 414,869 + RR42_RS22645 0.53 -0.2 415,388 + +1.2 415,527 + +0.8 415,527 + +0.5 415,687 + +0.8 415,688 - -0.0 415,870 + RR42_RS22650 0.28 -1.4 415,871 - RR42_RS22650 0.28 -0.7 415,912 + RR42_RS22650 0.32 -0.6 415,913 - RR42_RS22650 0.33 -0.1 415,921 + RR42_RS22650 0.33 -3.8 415,921 + RR42_RS22650 0.33 +0.2 415,922 - RR42_RS22650 0.33 -0.3 415,922 - RR42_RS22650 0.33 +0.9 415,922 - RR42_RS22650 0.33 -0.6 415,922 - RR42_RS22650 0.33 +0.2 416,065 + RR42_RS22650 0.48 -2.3 416,065 + RR42_RS22650 0.48 +0.1 416,158 + RR42_RS22650 0.57 -2.9 416,158 + RR42_RS22650 0.57 +0.7 416,159 - RR42_RS22650 0.57 +0.5 416,277 - RR42_RS22650 0.69 +0.3 416,302 + RR42_RS22650 0.72 +0.2
Or see this region's nucleotide sequence