Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22625 and RR42_RS22630 are separated by 71 nucleotides RR42_RS22630 and RR42_RS22635 are separated by 207 nucleotides
RR42_RS22625: RR42_RS22625 - thiolase, at 408,459 to 409,637
_RS22625
RR42_RS22630: RR42_RS22630 - 3-hydroxyacyl-CoA dehydrogenase, at 409,709 to 411,799
_RS22630
RR42_RS22635: RR42_RS22635 - membrane protein, at 412,007 to 413,080
_RS22635
Position (kb) 409
410
411
412 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 408.793 kb on + strand, within RR42_RS22625 at 408.793 kb on + strand, within RR42_RS22625 at 408.794 kb on - strand, within RR42_RS22625 at 408.880 kb on - strand, within RR42_RS22625 at 408.891 kb on + strand, within RR42_RS22625 at 408.892 kb on - strand, within RR42_RS22625 at 408.919 kb on - strand, within RR42_RS22625 at 409.183 kb on - strand, within RR42_RS22625 at 409.183 kb on - strand, within RR42_RS22625 at 409.326 kb on + strand, within RR42_RS22625 at 409.488 kb on + strand, within RR42_RS22625 at 409.488 kb on + strand, within RR42_RS22625 at 409.488 kb on + strand, within RR42_RS22625 at 409.489 kb on - strand, within RR42_RS22625 at 409.489 kb on - strand, within RR42_RS22625 at 409.610 kb on + strand at 409.611 kb on - strand at 409.651 kb on + strand at 410.726 kb on + strand, within RR42_RS22630 at 410.930 kb on - strand, within RR42_RS22630 at 411.164 kb on + strand, within RR42_RS22630 at 411.286 kb on + strand, within RR42_RS22630 at 411.286 kb on + strand, within RR42_RS22630 at 411.287 kb on - strand, within RR42_RS22630 at 411.404 kb on + strand, within RR42_RS22630 at 411.407 kb on + strand, within RR42_RS22630 at 411.614 kb on + strand at 411.614 kb on + strand at 411.614 kb on + strand at 411.867 kb on - strand at 411.867 kb on - strand at 411.872 kb on - strand at 412.341 kb on - strand, within RR42_RS22635 at 412.552 kb on + strand, within RR42_RS22635 at 412.552 kb on + strand, within RR42_RS22635 at 412.553 kb on - strand, within RR42_RS22635 at 412.562 kb on + strand, within RR42_RS22635
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 408,793 + RR42_RS22625 0.28 +0.5 408,793 + RR42_RS22625 0.28 +1.1 408,794 - RR42_RS22625 0.28 -0.2 408,880 - RR42_RS22625 0.36 -0.0 408,891 + RR42_RS22625 0.37 +0.7 408,892 - RR42_RS22625 0.37 +0.3 408,919 - RR42_RS22625 0.39 -1.5 409,183 - RR42_RS22625 0.61 -0.6 409,183 - RR42_RS22625 0.61 +1.2 409,326 + RR42_RS22625 0.74 +0.3 409,488 + RR42_RS22625 0.87 -1.3 409,488 + RR42_RS22625 0.87 +0.1 409,488 + RR42_RS22625 0.87 -0.7 409,489 - RR42_RS22625 0.87 +0.1 409,489 - RR42_RS22625 0.87 -1.0 409,610 + +2.2 409,611 - +1.0 409,651 + -2.3 410,726 + RR42_RS22630 0.49 +0.6 410,930 - RR42_RS22630 0.58 -0.4 411,164 + RR42_RS22630 0.70 -2.0 411,286 + RR42_RS22630 0.75 -0.6 411,286 + RR42_RS22630 0.75 +0.8 411,287 - RR42_RS22630 0.75 -1.3 411,404 + RR42_RS22630 0.81 -1.5 411,407 + RR42_RS22630 0.81 -0.8 411,614 + +3.2 411,614 + -0.3 411,614 + +1.0 411,867 - -0.7 411,867 - +0.6 411,872 - -0.5 412,341 - RR42_RS22635 0.31 -2.8 412,552 + RR42_RS22635 0.51 -0.6 412,552 + RR42_RS22635 0.51 +1.6 412,553 - RR42_RS22635 0.51 -1.0 412,562 + RR42_RS22635 0.52 +0.2
Or see this region's nucleotide sequence