Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22595 and RR42_RS22600 are separated by 133 nucleotides RR42_RS22600 and RR42_RS22605 are separated by 112 nucleotides RR42_RS22605 and RR42_RS22610 are separated by 2 nucleotides
RR42_RS22595: RR42_RS22595 - LysR family transcriptional regulator, at 403,539 to 404,465
_RS22595
RR42_RS22600: RR42_RS22600 - heavy metal resistance protein CzcE, at 404,599 to 404,964
_RS22600
RR42_RS22605: RR42_RS22605 - hypothetical protein, at 405,077 to 405,448
_RS22605
RR42_RS22610: RR42_RS22610 - toxin HipA, at 405,451 to 406,785
_RS22610
Position (kb) 404
405 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 403.645 kb on - strand, within RR42_RS22595 at 403.645 kb on - strand, within RR42_RS22595 at 403.859 kb on + strand, within RR42_RS22595 at 404.211 kb on - strand, within RR42_RS22595 at 404.263 kb on + strand, within RR42_RS22595 at 404.305 kb on + strand, within RR42_RS22595 at 404.463 kb on + strand at 404.464 kb on - strand at 404.516 kb on + strand at 404.581 kb on + strand at 404.582 kb on - strand at 404.582 kb on - strand at 404.782 kb on + strand, within RR42_RS22600 at 404.783 kb on - strand, within RR42_RS22600 at 404.783 kb on - strand, within RR42_RS22600 at 404.929 kb on + strand at 404.929 kb on + strand at 404.929 kb on + strand at 404.929 kb on + strand at 404.929 kb on + strand at 404.929 kb on + strand at 404.929 kb on + strand at 404.930 kb on - strand at 405.041 kb on - strand at 405.041 kb on - strand at 405.041 kb on - strand at 405.056 kb on + strand at 405.056 kb on + strand at 405.057 kb on - strand at 405.057 kb on - strand at 405.057 kb on - strand at 405.084 kb on - strand at 405.108 kb on + strand at 405.109 kb on - strand at 405.109 kb on - strand at 405.109 kb on - strand at 405.194 kb on + strand, within RR42_RS22605 at 405.229 kb on + strand, within RR42_RS22605 at 405.277 kb on - strand, within RR42_RS22605 at 405.277 kb on - strand, within RR42_RS22605 at 405.394 kb on + strand, within RR42_RS22605 at 405.447 kb on - strand at 405.447 kb on - strand at 405.447 kb on - strand at 405.544 kb on + strand at 405.544 kb on + strand at 405.622 kb on + strand, within RR42_RS22610 at 405.622 kb on + strand, within RR42_RS22610 at 405.623 kb on - strand, within RR42_RS22610 at 405.892 kb on + strand, within RR42_RS22610 at 405.892 kb on + strand, within RR42_RS22610 at 405.892 kb on + strand, within RR42_RS22610 at 405.892 kb on + strand, within RR42_RS22610 at 405.892 kb on + strand, within RR42_RS22610 at 405.893 kb on - strand, within RR42_RS22610 at 405.911 kb on + strand, within RR42_RS22610 at 405.911 kb on + strand, within RR42_RS22610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 403,645 - RR42_RS22595 0.11 -2.9 403,645 - RR42_RS22595 0.11 +0.9 403,859 + RR42_RS22595 0.35 +0.1 404,211 - RR42_RS22595 0.72 +0.5 404,263 + RR42_RS22595 0.78 -2.1 404,305 + RR42_RS22595 0.83 +0.3 404,463 + -0.9 404,464 - +0.7 404,516 + +1.2 404,581 + -1.9 404,582 - +1.0 404,582 - +0.6 404,782 + RR42_RS22600 0.50 -0.6 404,783 - RR42_RS22600 0.50 -0.8 404,783 - RR42_RS22600 0.50 -0.9 404,929 + +0.8 404,929 + -0.5 404,929 + +0.1 404,929 + +1.6 404,929 + -0.7 404,929 + +0.9 404,929 + +1.9 404,930 - -0.3 405,041 - -0.0 405,041 - +0.9 405,041 - -0.0 405,056 + +2.4 405,056 + -0.8 405,057 - +0.0 405,057 - +0.1 405,057 - +1.2 405,084 - +0.7 405,108 + -0.6 405,109 - +1.4 405,109 - -1.3 405,109 - +1.2 405,194 + RR42_RS22605 0.31 -0.8 405,229 + RR42_RS22605 0.41 +1.3 405,277 - RR42_RS22605 0.54 -0.7 405,277 - RR42_RS22605 0.54 -0.9 405,394 + RR42_RS22605 0.85 -0.0 405,447 - +1.4 405,447 - -0.4 405,447 - -1.4 405,544 + -2.2 405,544 + +0.1 405,622 + RR42_RS22610 0.13 +3.1 405,622 + RR42_RS22610 0.13 -0.4 405,623 - RR42_RS22610 0.13 +1.6 405,892 + RR42_RS22610 0.33 -1.1 405,892 + RR42_RS22610 0.33 -1.5 405,892 + RR42_RS22610 0.33 +0.2 405,892 + RR42_RS22610 0.33 -1.0 405,892 + RR42_RS22610 0.33 -0.3 405,893 - RR42_RS22610 0.33 +0.7 405,911 + RR42_RS22610 0.34 +0.2 405,911 + RR42_RS22610 0.34 +2.5
Or see this region's nucleotide sequence