Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22335 and RR42_RS22340 are separated by 32 nucleotides RR42_RS22340 and RR42_RS22345 are separated by 233 nucleotides RR42_RS22345 and RR42_RS22350 are separated by 150 nucleotides
RR42_RS22335: RR42_RS22335 - hemagglutinin, at 351,215 to 352,492
_RS22335
RR42_RS22340: RR42_RS22340 - peptidase, at 352,525 to 353,808
_RS22340
RR42_RS22345: RR42_RS22345 - UDP-N-acetylmuramate--alanine ligase, at 354,042 to 354,395
_RS22345
RR42_RS22350: RR42_RS22350 - transporter, at 354,546 to 354,974
_RS22350
Position (kb) 352
353
354 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 351.559 kb on + strand, within RR42_RS22335 at 351.559 kb on + strand, within RR42_RS22335 at 351.559 kb on + strand, within RR42_RS22335 at 351.559 kb on + strand, within RR42_RS22335 at 352.031 kb on - strand, within RR42_RS22335 at 352.031 kb on - strand, within RR42_RS22335 at 352.032 kb on - strand, within RR42_RS22335 at 352.150 kb on + strand, within RR42_RS22335 at 352.237 kb on + strand, within RR42_RS22335 at 352.237 kb on + strand, within RR42_RS22335 at 352.238 kb on - strand, within RR42_RS22335 at 352.494 kb on - strand at 352.524 kb on - strand at 352.524 kb on - strand at 352.590 kb on - strand at 352.592 kb on + strand at 352.592 kb on + strand at 352.592 kb on + strand at 352.593 kb on - strand at 352.593 kb on - strand at 352.808 kb on + strand, within RR42_RS22340 at 352.828 kb on + strand, within RR42_RS22340 at 353.088 kb on + strand, within RR42_RS22340 at 353.089 kb on - strand, within RR42_RS22340 at 353.089 kb on - strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.376 kb on - strand, within RR42_RS22340 at 353.475 kb on - strand, within RR42_RS22340 at 353.599 kb on + strand, within RR42_RS22340 at 353.638 kb on + strand, within RR42_RS22340 at 353.692 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.960 kb on - strand at 353.960 kb on - strand at 353.960 kb on - strand at 354.068 kb on - strand at 354.206 kb on + strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.318 kb on + strand, within RR42_RS22345 at 354.319 kb on - strand, within RR42_RS22345 at 354.381 kb on + strand at 354.382 kb on - strand at 354.458 kb on + strand at 354.459 kb on - strand at 354.578 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 351,559 + RR42_RS22335 0.27 -0.8 351,559 + RR42_RS22335 0.27 +1.0 351,559 + RR42_RS22335 0.27 -0.0 351,559 + RR42_RS22335 0.27 -2.9 352,031 - RR42_RS22335 0.64 +1.1 352,031 - RR42_RS22335 0.64 -0.7 352,032 - RR42_RS22335 0.64 -1.7 352,150 + RR42_RS22335 0.73 -0.2 352,237 + RR42_RS22335 0.80 -2.3 352,237 + RR42_RS22335 0.80 -2.5 352,238 - RR42_RS22335 0.80 +1.1 352,494 - +0.5 352,524 - +0.5 352,524 - +1.2 352,590 - -3.1 352,592 + -3.3 352,592 + +2.0 352,592 + +1.0 352,593 - -0.2 352,593 - -1.1 352,808 + RR42_RS22340 0.22 +0.6 352,828 + RR42_RS22340 0.24 -0.9 353,088 + RR42_RS22340 0.44 -0.6 353,089 - RR42_RS22340 0.44 +1.6 353,089 - RR42_RS22340 0.44 +1.2 353,307 + RR42_RS22340 0.61 -0.6 353,307 + RR42_RS22340 0.61 +0.8 353,307 + RR42_RS22340 0.61 +0.1 353,307 + RR42_RS22340 0.61 -2.1 353,307 + RR42_RS22340 0.61 +0.5 353,307 + RR42_RS22340 0.61 +0.8 353,307 + RR42_RS22340 0.61 +2.4 353,308 - RR42_RS22340 0.61 -2.3 353,308 - RR42_RS22340 0.61 +0.6 353,308 - RR42_RS22340 0.61 -0.0 353,308 - RR42_RS22340 0.61 +0.6 353,308 - RR42_RS22340 0.61 +0.4 353,308 - RR42_RS22340 0.61 +0.2 353,308 - RR42_RS22340 0.61 +1.3 353,308 - RR42_RS22340 0.61 -0.6 353,376 - RR42_RS22340 0.66 -0.5 353,475 - RR42_RS22340 0.74 +1.7 353,599 + RR42_RS22340 0.84 -0.5 353,638 + RR42_RS22340 0.87 +0.4 353,692 + +0.7 353,959 + -0.0 353,959 + +1.3 353,959 + +0.4 353,959 + -0.8 353,960 - -2.9 353,960 - -1.0 353,960 - -1.7 354,068 - -2.1 354,206 + RR42_RS22345 0.46 -1.8 354,207 - RR42_RS22345 0.47 -0.4 354,207 - RR42_RS22345 0.47 +1.3 354,318 + RR42_RS22345 0.78 -0.0 354,319 - RR42_RS22345 0.78 +1.8 354,381 + +1.8 354,382 - -0.8 354,458 + -1.5 354,459 - +0.6 354,578 + +0.3
Or see this region's nucleotide sequence