Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS22320

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS22310 and RR42_RS22315 are separated by 202 nucleotidesRR42_RS22315 and RR42_RS22320 are separated by 35 nucleotidesRR42_RS22320 and RR42_RS22325 overlap by 4 nucleotides RR42_RS22310: RR42_RS22310 - LysR family transcriptional regulator, at 346,289 to 347,188 _RS22310 RR42_RS22315: RR42_RS22315 - LysR family transcriptional regulator, at 347,391 to 347,831 _RS22315 RR42_RS22320: RR42_RS22320 - MFS transporter, at 347,867 to 349,171 _RS22320 RR42_RS22325: RR42_RS22325 - hypothetical protein, at 349,168 to 350,265 _RS22325 Position (kb) 347 348 349 350Strain fitness (log2 ratio) -2 -1 0 1 2at 347.069 kb on - strand, within RR42_RS22310at 347.069 kb on - strand, within RR42_RS22310at 347.070 kb on + strand, within RR42_RS22310at 347.070 kb on + strand, within RR42_RS22310at 347.071 kb on - strand, within RR42_RS22310at 347.071 kb on - strand, within RR42_RS22310at 347.200 kb on + strandat 347.219 kb on + strandat 347.219 kb on + strandat 347.254 kb on - strandat 347.259 kb on + strandat 347.545 kb on - strand, within RR42_RS22315at 347.653 kb on + strand, within RR42_RS22315at 347.654 kb on - strand, within RR42_RS22315at 347.654 kb on - strand, within RR42_RS22315at 347.654 kb on - strand, within RR42_RS22315at 347.654 kb on - strand, within RR42_RS22315at 347.675 kb on + strand, within RR42_RS22315at 347.784 kb on + strand, within RR42_RS22315at 347.785 kb on - strand, within RR42_RS22315at 347.922 kb on + strandat 347.922 kb on + strandat 347.923 kb on - strandat 347.923 kb on - strandat 347.923 kb on - strandat 347.923 kb on - strandat 347.923 kb on - strandat 348.354 kb on + strand, within RR42_RS22320at 348.354 kb on + strand, within RR42_RS22320at 348.354 kb on + strand, within RR42_RS22320at 348.355 kb on - strand, within RR42_RS22320at 348.355 kb on - strand, within RR42_RS22320at 348.355 kb on - strand, within RR42_RS22320at 348.685 kb on - strand, within RR42_RS22320at 348.732 kb on + strand, within RR42_RS22320at 348.750 kb on + strand, within RR42_RS22320at 348.750 kb on + strand, within RR42_RS22320at 348.750 kb on + strand, within RR42_RS22320at 348.751 kb on - strand, within RR42_RS22320at 348.808 kb on - strand, within RR42_RS22320at 348.808 kb on - strand, within RR42_RS22320at 348.939 kb on + strand, within RR42_RS22320at 348.940 kb on - strand, within RR42_RS22320at 349.044 kb on + strandat 349.045 kb on - strandat 349.056 kb on + strandat 349.056 kb on + strandat 349.056 kb on + strandat 349.056 kb on + strandat 349.056 kb on + strandat 349.056 kb on + strandat 349.057 kb on - strandat 349.057 kb on - strandat 349.068 kb on - strandat 349.355 kb on + strand, within RR42_RS22325at 349.355 kb on + strand, within RR42_RS22325at 349.355 kb on + strand, within RR42_RS22325at 349.355 kb on + strand, within RR42_RS22325at 349.356 kb on - strand, within RR42_RS22325at 349.380 kb on + strand, within RR42_RS22325at 349.465 kb on - strand, within RR42_RS22325at 349.517 kb on + strand, within RR42_RS22325at 349.647 kb on - strand, within RR42_RS22325at 349.787 kb on + strand, within RR42_RS22325at 349.787 kb on + strand, within RR42_RS22325at 349.788 kb on - strand, within RR42_RS22325at 349.788 kb on - strand, within RR42_RS22325at 349.788 kb on - strand, within RR42_RS22325at 349.797 kb on - strand, within RR42_RS22325at 349.878 kb on + strand, within RR42_RS22325at 349.879 kb on + strand, within RR42_RS22325at 349.914 kb on - strand, within RR42_RS22325at 350.051 kb on + strand, within RR42_RS22325at 350.051 kb on + strand, within RR42_RS22325at 350.051 kb on + strand, within RR42_RS22325at 350.069 kb on + strand, within RR42_RS22325at 350.070 kb on - strand, within RR42_RS22325

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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347,069 - RR42_RS22310 0.87 -0.5
347,069 - RR42_RS22310 0.87 -2.5
347,070 + RR42_RS22310 0.87 -2.7
347,070 + RR42_RS22310 0.87 +1.0
347,071 - RR42_RS22310 0.87 -0.3
347,071 - RR42_RS22310 0.87 +0.3
347,200 + +0.5
347,219 + +0.2
347,219 + -0.2
347,254 - -0.0
347,259 + -0.3
347,545 - RR42_RS22315 0.35 -1.1
347,653 + RR42_RS22315 0.59 -1.7
347,654 - RR42_RS22315 0.60 -0.3
347,654 - RR42_RS22315 0.60 +1.5
347,654 - RR42_RS22315 0.60 -0.0
347,654 - RR42_RS22315 0.60 -0.6
347,675 + RR42_RS22315 0.64 +1.3
347,784 + RR42_RS22315 0.89 -0.2
347,785 - RR42_RS22315 0.89 +1.6
347,922 + -1.7
347,922 + -0.5
347,923 - -1.6
347,923 - +1.5
347,923 - -0.2
347,923 - -0.6
347,923 - -0.3
348,354 + RR42_RS22320 0.37 -0.7
348,354 + RR42_RS22320 0.37 -2.4
348,354 + RR42_RS22320 0.37 +0.4
348,355 - RR42_RS22320 0.37 +1.9
348,355 - RR42_RS22320 0.37 +0.1
348,355 - RR42_RS22320 0.37 -2.3
348,685 - RR42_RS22320 0.63 -0.0
348,732 + RR42_RS22320 0.66 -1.2
348,750 + RR42_RS22320 0.68 -0.3
348,750 + RR42_RS22320 0.68 +0.1
348,750 + RR42_RS22320 0.68 -1.0
348,751 - RR42_RS22320 0.68 +0.2
348,808 - RR42_RS22320 0.72 -0.9
348,808 - RR42_RS22320 0.72 -0.5
348,939 + RR42_RS22320 0.82 -1.0
348,940 - RR42_RS22320 0.82 -0.6
349,044 + +1.5
349,045 - +1.8
349,056 + -0.1
349,056 + -0.9
349,056 + +0.2
349,056 + +0.1
349,056 + +0.2
349,056 + -0.4
349,057 - -0.5
349,057 - +1.5
349,068 - +0.4
349,355 + RR42_RS22325 0.17 +0.6
349,355 + RR42_RS22325 0.17 -0.2
349,355 + RR42_RS22325 0.17 +0.1
349,355 + RR42_RS22325 0.17 -1.1
349,356 - RR42_RS22325 0.17 -1.1
349,380 + RR42_RS22325 0.19 -1.5
349,465 - RR42_RS22325 0.27 -1.4
349,517 + RR42_RS22325 0.32 -0.5
349,647 - RR42_RS22325 0.44 -0.7
349,787 + RR42_RS22325 0.56 +0.3
349,787 + RR42_RS22325 0.56 +0.7
349,788 - RR42_RS22325 0.56 +0.6
349,788 - RR42_RS22325 0.56 +0.6
349,788 - RR42_RS22325 0.56 -2.0
349,797 - RR42_RS22325 0.57 +0.6
349,878 + RR42_RS22325 0.65 +0.6
349,879 + RR42_RS22325 0.65 +0.0
349,914 - RR42_RS22325 0.68 +1.6
350,051 + RR42_RS22325 0.80 -0.2
350,051 + RR42_RS22325 0.80 +0.1
350,051 + RR42_RS22325 0.80 -1.8
350,069 + RR42_RS22325 0.82 -0.6
350,070 - RR42_RS22325 0.82 -0.6

Or see this region's nucleotide sequence