Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22280 and RR42_RS22285 are separated by 72 nucleotides RR42_RS22285 and RR42_RS22290 are separated by 123 nucleotides RR42_RS22290 and RR42_RS22295 are separated by 50 nucleotides RR42_RS22295 and RR42_RS22300 are separated by 226 nucleotides
RR42_RS22280: RR42_RS22280 - cupin, at 340,023 to 340,478
_RS22280
RR42_RS22285: RR42_RS22285 - methionine sulfoxide reductase A, at 340,551 to 341,291
_RS22285
RR42_RS22290: RR42_RS22290 - cytochrome C biogenesis protein, at 341,415 to 343,223
_RS22290
RR42_RS22295: RR42_RS22295 - methionine sulfoxide reductase B, at 343,274 to 343,813
_RS22295
RR42_RS22300: RR42_RS22300 - chemotaxis protein CheY, at 344,040 to 344,762
_RS22300
Position (kb) 341
342
343
344 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 340.437 kb on + strand at 340.651 kb on + strand, within RR42_RS22285 at 340.651 kb on + strand, within RR42_RS22285 at 340.652 kb on - strand, within RR42_RS22285 at 340.690 kb on + strand, within RR42_RS22285 at 340.691 kb on - strand, within RR42_RS22285 at 340.779 kb on - strand, within RR42_RS22285 at 340.780 kb on + strand, within RR42_RS22285 at 340.780 kb on + strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.835 kb on - strand, within RR42_RS22285 at 340.994 kb on - strand, within RR42_RS22285 at 341.322 kb on + strand at 341.323 kb on - strand at 341.355 kb on + strand at 341.435 kb on - strand at 341.435 kb on - strand at 341.513 kb on - strand at 341.776 kb on + strand, within RR42_RS22290 at 341.776 kb on + strand, within RR42_RS22290 at 341.776 kb on + strand, within RR42_RS22290 at 341.777 kb on - strand, within RR42_RS22290 at 341.777 kb on - strand, within RR42_RS22290 at 341.845 kb on + strand, within RR42_RS22290 at 341.845 kb on + strand, within RR42_RS22290 at 341.845 kb on + strand, within RR42_RS22290 at 342.001 kb on + strand, within RR42_RS22290 at 342.055 kb on + strand, within RR42_RS22290 at 342.100 kb on + strand, within RR42_RS22290 at 342.100 kb on + strand, within RR42_RS22290 at 342.100 kb on + strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.286 kb on + strand, within RR42_RS22290 at 342.287 kb on - strand, within RR42_RS22290 at 342.500 kb on + strand, within RR42_RS22290 at 343.202 kb on - strand at 343.211 kb on + strand at 343.211 kb on + strand at 343.436 kb on + strand, within RR42_RS22295 at 343.659 kb on - strand, within RR42_RS22295 at 343.835 kb on + strand at 343.835 kb on + strand at 343.893 kb on + strand at 343.894 kb on - strand at 343.894 kb on - strand at 343.934 kb on + strand at 343.972 kb on - strand at 344.095 kb on + strand at 344.095 kb on + strand at 344.096 kb on - strand at 344.096 kb on - strand at 344.124 kb on + strand, within RR42_RS22300 at 344.125 kb on - strand, within RR42_RS22300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 340,437 + -0.1 340,651 + RR42_RS22285 0.13 +0.1 340,651 + RR42_RS22285 0.13 +0.3 340,652 - RR42_RS22285 0.14 -0.5 340,690 + RR42_RS22285 0.19 +0.0 340,691 - RR42_RS22285 0.19 -0.4 340,779 - RR42_RS22285 0.31 -1.0 340,780 + RR42_RS22285 0.31 +0.4 340,780 + RR42_RS22285 0.31 -2.2 340,781 - RR42_RS22285 0.31 -1.1 340,781 - RR42_RS22285 0.31 +0.6 340,781 - RR42_RS22285 0.31 -2.3 340,835 - RR42_RS22285 0.38 -1.6 340,994 - RR42_RS22285 0.60 -0.8 341,322 + -0.9 341,323 - -0.5 341,355 + -2.1 341,435 - +0.5 341,435 - +0.0 341,513 - -0.7 341,776 + RR42_RS22290 0.20 +0.2 341,776 + RR42_RS22290 0.20 +0.6 341,776 + RR42_RS22290 0.20 -0.9 341,777 - RR42_RS22290 0.20 +0.8 341,777 - RR42_RS22290 0.20 -1.3 341,845 + RR42_RS22290 0.24 +2.8 341,845 + RR42_RS22290 0.24 +3.3 341,845 + RR42_RS22290 0.24 +0.5 342,001 + RR42_RS22290 0.32 +1.5 342,055 + RR42_RS22290 0.35 +0.9 342,100 + RR42_RS22290 0.38 -0.1 342,100 + RR42_RS22290 0.38 -0.5 342,100 + RR42_RS22290 0.38 +0.1 342,254 - RR42_RS22290 0.46 +0.8 342,254 - RR42_RS22290 0.46 -1.0 342,254 - RR42_RS22290 0.46 -2.0 342,254 - RR42_RS22290 0.46 +0.6 342,286 + RR42_RS22290 0.48 -0.5 342,287 - RR42_RS22290 0.48 +1.2 342,500 + RR42_RS22290 0.60 -1.5 343,202 - -1.7 343,211 + -2.0 343,211 + -1.8 343,436 + RR42_RS22295 0.30 +0.4 343,659 - RR42_RS22295 0.71 -0.5 343,835 + -0.8 343,835 + -1.3 343,893 + +0.4 343,894 - +1.2 343,894 - -0.6 343,934 + +0.4 343,972 - -2.4 344,095 + +0.8 344,095 + -0.4 344,096 - +0.2 344,096 - +0.7 344,124 + RR42_RS22300 0.12 -1.1 344,125 - RR42_RS22300 0.12 +0.6
Or see this region's nucleotide sequence